The relationship between structural changes in paraspinal muscles and intervertebral disc and facet joint degeneration in the lumbar spine of rats
Abstract Background Degenerative spine disease is one of the largest causes of disability worldwide and has a multifactorial aetiology. Determining the leading causes of this multifactorial disease could help create new treatment approaches. Purpose Study the impact of degenerative changes in the pa...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2024-01-01
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| Series: | Journal of Orthopaedic Surgery and Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13018-024-04548-8 |
| _version_ | 1797355683997286400 |
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| author | Artem Skidanov Nataliya Ashukina Valentyna Maltseva Mykyta Skidanov Zinaida Danyshchuk Volodymyr Radchenko |
| author_facet | Artem Skidanov Nataliya Ashukina Valentyna Maltseva Mykyta Skidanov Zinaida Danyshchuk Volodymyr Radchenko |
| author_sort | Artem Skidanov |
| collection | DOAJ |
| description | Abstract Background Degenerative spine disease is one of the largest causes of disability worldwide and has a multifactorial aetiology. Determining the leading causes of this multifactorial disease could help create new treatment approaches. Purpose Study the impact of degenerative changes in the paraspinal muscles caused by local (prolonged compression) or systemic (high-fat diet) factors on the structure of the intervertebral discs (IVDs) and facet joints of the lumbar spine in rats. Methods The study was conducted using two animal models to create degenerative changes in the paraspinal muscles of 10 white laboratory rats for 90 days and five control rats: 1) high-fat diet model (model 1) involved keeping the rats on a high calorie diet; 2) compression model (model 2) involved binding the paraspinal muscles from L2 to S1 using non-absorbable sutures. Histological analysis for the facet joints and IVDs of rats (at the L1–L4 level) with semi-quantitative analysis of the structure conducted used by degeneration grading system for IVDs and cartilage degeneration score (OARSI) for facet joint. Results In both models, 90 days after the experiment, the degenerative changes observed in the rats’ IVDs were more severe in the annulus fibrosus than in the nucleus pulposus. The height of the IVD in model 1 did not differ from the control group, but in the model 2 was 1.3 times greater (p < 0.001) compared with control. Degenerative changes in the IVD were scored out 5.3 ± 1.7 in model 1 and 5.32 ± 2.1 in model 2 of a possible 16. The height of the articular cartilage of the facet joints was smaller by 1.5 times (p < 0.001) and 1.4 times (p < 0.001) in model 1 and model 2, respectively, compared to the control. Degenerative changes of facet joint were scored out 3.7 ± 0.6 in model 1 and 3.8 ± 0.6 in model 2 of five points according to the cartilage degeneration score. Conclusions It was determined that rats who had structural changes in the lumbar paraspinal muscles as a result of being kept on a high-fat diet or subjected to prolonged compression for 90 days, showed degenerative changes in intervertebral discs and osteoarthritis in facet joints of lumbar spine. |
| first_indexed | 2024-03-08T14:14:38Z |
| format | Article |
| id | doaj.art-eb7ccd683c1f4bd2b61beb12ee09f774 |
| institution | Directory Open Access Journal |
| issn | 1749-799X |
| language | English |
| last_indexed | 2024-03-08T14:14:38Z |
| publishDate | 2024-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Orthopaedic Surgery and Research |
| spelling | doaj.art-eb7ccd683c1f4bd2b61beb12ee09f7742024-01-14T12:30:18ZengBMCJournal of Orthopaedic Surgery and Research1749-799X2024-01-011911910.1186/s13018-024-04548-8The relationship between structural changes in paraspinal muscles and intervertebral disc and facet joint degeneration in the lumbar spine of ratsArtem Skidanov0Nataliya Ashukina1Valentyna Maltseva2Mykyta Skidanov3Zinaida Danyshchuk4Volodymyr Radchenko5Laboratory of Connective Tissue Morphology, Sytenko Institute of Spine and Joint Pathology National Academy of Medical Sciences of UkraineLaboratory of Connective Tissue Morphology, Sytenko Institute of Spine and Joint Pathology National Academy of Medical Sciences of UkraineLaboratory of Connective Tissue Morphology, Sytenko Institute of Spine and Joint Pathology National Academy of Medical Sciences of UkraineLaboratory of Connective Tissue Morphology, Sytenko Institute of Spine and Joint Pathology National Academy of Medical Sciences of UkraineLaboratory of Connective Tissue Morphology, Sytenko Institute of Spine and Joint Pathology National Academy of Medical Sciences of UkraineLaboratory of Connective Tissue Morphology, Sytenko Institute of Spine and Joint Pathology National Academy of Medical Sciences of UkraineAbstract Background Degenerative spine disease is one of the largest causes of disability worldwide and has a multifactorial aetiology. Determining the leading causes of this multifactorial disease could help create new treatment approaches. Purpose Study the impact of degenerative changes in the paraspinal muscles caused by local (prolonged compression) or systemic (high-fat diet) factors on the structure of the intervertebral discs (IVDs) and facet joints of the lumbar spine in rats. Methods The study was conducted using two animal models to create degenerative changes in the paraspinal muscles of 10 white laboratory rats for 90 days and five control rats: 1) high-fat diet model (model 1) involved keeping the rats on a high calorie diet; 2) compression model (model 2) involved binding the paraspinal muscles from L2 to S1 using non-absorbable sutures. Histological analysis for the facet joints and IVDs of rats (at the L1–L4 level) with semi-quantitative analysis of the structure conducted used by degeneration grading system for IVDs and cartilage degeneration score (OARSI) for facet joint. Results In both models, 90 days after the experiment, the degenerative changes observed in the rats’ IVDs were more severe in the annulus fibrosus than in the nucleus pulposus. The height of the IVD in model 1 did not differ from the control group, but in the model 2 was 1.3 times greater (p < 0.001) compared with control. Degenerative changes in the IVD were scored out 5.3 ± 1.7 in model 1 and 5.32 ± 2.1 in model 2 of a possible 16. The height of the articular cartilage of the facet joints was smaller by 1.5 times (p < 0.001) and 1.4 times (p < 0.001) in model 1 and model 2, respectively, compared to the control. Degenerative changes of facet joint were scored out 3.7 ± 0.6 in model 1 and 3.8 ± 0.6 in model 2 of five points according to the cartilage degeneration score. Conclusions It was determined that rats who had structural changes in the lumbar paraspinal muscles as a result of being kept on a high-fat diet or subjected to prolonged compression for 90 days, showed degenerative changes in intervertebral discs and osteoarthritis in facet joints of lumbar spine.https://doi.org/10.1186/s13018-024-04548-8RatsAnnulus fibrosusCartilageIntervertebral discFacet joint osteoarthritisZygapophyseal joint |
| spellingShingle | Artem Skidanov Nataliya Ashukina Valentyna Maltseva Mykyta Skidanov Zinaida Danyshchuk Volodymyr Radchenko The relationship between structural changes in paraspinal muscles and intervertebral disc and facet joint degeneration in the lumbar spine of rats Journal of Orthopaedic Surgery and Research Rats Annulus fibrosus Cartilage Intervertebral disc Facet joint osteoarthritis Zygapophyseal joint |
| title | The relationship between structural changes in paraspinal muscles and intervertebral disc and facet joint degeneration in the lumbar spine of rats |
| title_full | The relationship between structural changes in paraspinal muscles and intervertebral disc and facet joint degeneration in the lumbar spine of rats |
| title_fullStr | The relationship between structural changes in paraspinal muscles and intervertebral disc and facet joint degeneration in the lumbar spine of rats |
| title_full_unstemmed | The relationship between structural changes in paraspinal muscles and intervertebral disc and facet joint degeneration in the lumbar spine of rats |
| title_short | The relationship between structural changes in paraspinal muscles and intervertebral disc and facet joint degeneration in the lumbar spine of rats |
| title_sort | relationship between structural changes in paraspinal muscles and intervertebral disc and facet joint degeneration in the lumbar spine of rats |
| topic | Rats Annulus fibrosus Cartilage Intervertebral disc Facet joint osteoarthritis Zygapophyseal joint |
| url | https://doi.org/10.1186/s13018-024-04548-8 |
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