Transcriptional Regulation of zma-<i>MIR528a</i> by Action of Nitrate and Auxin in Maize
In recent years, miR528, a monocot-specific miRNA, has been assigned multifaceted roles during development and stress response in several plant species. However, the transcription regulation and the molecular mechanisms controlling <i>MIR528</i> expression in maize are still poorly explo...
Main Authors: | , , , , , |
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Format: | Article |
Language: | English |
Published: |
MDPI AG
2022-12-01
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Series: | International Journal of Molecular Sciences |
Subjects: | |
Online Access: | https://www.mdpi.com/1422-0067/23/24/15718 |
Summary: | In recent years, miR528, a monocot-specific miRNA, has been assigned multifaceted roles during development and stress response in several plant species. However, the transcription regulation and the molecular mechanisms controlling <i>MIR528</i> expression in maize are still poorly explored. Here we analyzed the zma-<i>MIR528a</i> promoter region and found conserved transcription factor binding sites related to diverse signaling pathways, including the nitrate (TGA1/4) and auxin (AuxRE) response networks. Accumulation of both pre-miR528a and mature miR528 was up-regulated by exogenous nitrate and auxin treatments during imbibition, germination, and maize seedling establishment. Functional promoter analyses demonstrated that TGA1/4 and AuxRE sites are required for transcriptional induction by both stimuli. Overall, our findings of the nitrogen- and auxin-induced zma-<i>MIR528a</i> expression through <i>cis</i>-regulatory elements in its promoter contribute to the knowledge of miR528 regulome. |
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ISSN: | 1661-6596 1422-0067 |