Chemical inhibition of the contaminant Lactobacillus fermentum from distilleries producing fuel bioethanol
The purpose of this study was to determine the Minimum Inhibitory Concentration (MIC) of pure or mixed chemicals for Saccharomyces cerevisiae and Lactobacillus fermentum in the samples isolated from distilleries with serious bacterial contamination problems. The biocides, which showed the best resul...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Instituto de Tecnologia do Paraná (Tecpar)
2014-06-01
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| Series: | Brazilian Archives of Biology and Technology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000300019&lng=en&tlng=en |
| _version_ | 1818196488217427968 |
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| author | Pedro de Oliva Neto Fabíola Aliaga de Lima Ketrin Cristina da Silva Douglas Fernandes da Silva Ana Flavia Azevedo Carvalho Catarina dos Santos |
| author_facet | Pedro de Oliva Neto Fabíola Aliaga de Lima Ketrin Cristina da Silva Douglas Fernandes da Silva Ana Flavia Azevedo Carvalho Catarina dos Santos |
| author_sort | Pedro de Oliva Neto |
| collection | DOAJ |
| description | The purpose of this study was to determine the Minimum Inhibitory Concentration (MIC) of pure or mixed chemicals for Saccharomyces cerevisiae and Lactobacillus fermentum in the samples isolated from distilleries with serious bacterial contamination problems. The biocides, which showed the best results were: 3,4,4' trichlorocarbanilide (TCC), tested at pH 4.0 (MIC = 3.12 mg/l), TCC with benzethonium chloride (CBe) at pH 6.0 (MIC = 3.12 mg/l) and TCC mixed with benzalkonium chloride (CBa) at pH 6.0 (MIC = 1.53 mg /l). If CBa was used in sugar cane milling in 1:1 ratio with TCC, a 8 times reduction of CBa was possible. This formulation also should be tested in fermentation steps since it was more difficult for the bacterium to develop resistance to biocide. There was no inhibition of S. cerevisiae and there were only antibiotics as an option to bacterial control of fuel ethanol fermentation by S. cerevisiae. |
| first_indexed | 2024-12-12T01:34:52Z |
| format | Article |
| id | doaj.art-eb7e857f4fee454da3e6c63f0c308b74 |
| institution | Directory Open Access Journal |
| issn | 1678-4324 |
| language | English |
| last_indexed | 2024-12-12T01:34:52Z |
| publishDate | 2014-06-01 |
| publisher | Instituto de Tecnologia do Paraná (Tecpar) |
| record_format | Article |
| series | Brazilian Archives of Biology and Technology |
| spelling | doaj.art-eb7e857f4fee454da3e6c63f0c308b742022-12-22T00:42:53ZengInstituto de Tecnologia do Paraná (Tecpar)Brazilian Archives of Biology and Technology1678-43242014-06-0157344144710.1590/S1516-8913201401214S1516-89132014000300019Chemical inhibition of the contaminant Lactobacillus fermentum from distilleries producing fuel bioethanolPedro de Oliva Neto0Fabíola Aliaga de Lima1Ketrin Cristina da Silva2Douglas Fernandes da Silva3Ana Flavia Azevedo Carvalho4Catarina dos Santos5Universidade Estadual PaulistaUniversidade Estadual PaulistaUniversidade Estadual PaulistaUniversidade Estadual PaulistaUniversidade Estadual de CampinasUniversidade Estadual PaulistaThe purpose of this study was to determine the Minimum Inhibitory Concentration (MIC) of pure or mixed chemicals for Saccharomyces cerevisiae and Lactobacillus fermentum in the samples isolated from distilleries with serious bacterial contamination problems. The biocides, which showed the best results were: 3,4,4' trichlorocarbanilide (TCC), tested at pH 4.0 (MIC = 3.12 mg/l), TCC with benzethonium chloride (CBe) at pH 6.0 (MIC = 3.12 mg/l) and TCC mixed with benzalkonium chloride (CBa) at pH 6.0 (MIC = 1.53 mg /l). If CBa was used in sugar cane milling in 1:1 ratio with TCC, a 8 times reduction of CBa was possible. This formulation also should be tested in fermentation steps since it was more difficult for the bacterium to develop resistance to biocide. There was no inhibition of S. cerevisiae and there were only antibiotics as an option to bacterial control of fuel ethanol fermentation by S. cerevisiae.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000300019&lng=en&tlng=enEthanollactic acid bacteriaSaccharomyces cerevisiaeLactobacillus fermentum |
| spellingShingle | Pedro de Oliva Neto Fabíola Aliaga de Lima Ketrin Cristina da Silva Douglas Fernandes da Silva Ana Flavia Azevedo Carvalho Catarina dos Santos Chemical inhibition of the contaminant Lactobacillus fermentum from distilleries producing fuel bioethanol Brazilian Archives of Biology and Technology Ethanol lactic acid bacteria Saccharomyces cerevisiae Lactobacillus fermentum |
| title | Chemical inhibition of the contaminant Lactobacillus fermentum from distilleries producing fuel bioethanol |
| title_full | Chemical inhibition of the contaminant Lactobacillus fermentum from distilleries producing fuel bioethanol |
| title_fullStr | Chemical inhibition of the contaminant Lactobacillus fermentum from distilleries producing fuel bioethanol |
| title_full_unstemmed | Chemical inhibition of the contaminant Lactobacillus fermentum from distilleries producing fuel bioethanol |
| title_short | Chemical inhibition of the contaminant Lactobacillus fermentum from distilleries producing fuel bioethanol |
| title_sort | chemical inhibition of the contaminant lactobacillus fermentum from distilleries producing fuel bioethanol |
| topic | Ethanol lactic acid bacteria Saccharomyces cerevisiae Lactobacillus fermentum |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000300019&lng=en&tlng=en |
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