Cytotoxic and molecular assessment with copper and iron nanocomposite, act as a soft eradicator against cancer cells
The composites are the materials, which have either one or more combination with other material and exhibit extraordinary chemical characteristics. The present work describes the formation of composite of copper and iron metal oxide (CuFeO2). The synthesized powder materials are in nanorange. The na...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-04-01
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| Series: | Journal of King Saud University: Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1018364722000891 |
| _version_ | 1818338356052885504 |
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| author | Rizwan Wahab Farheen Khan Javed Ahmad Abdulaziz A. Al-Khedhairy |
| author_facet | Rizwan Wahab Farheen Khan Javed Ahmad Abdulaziz A. Al-Khedhairy |
| author_sort | Rizwan Wahab |
| collection | DOAJ |
| description | The composites are the materials, which have either one or more combination with other material and exhibit extraordinary chemical characteristics. The present work describes the formation of composite of copper and iron metal oxide (CuFeO2). The synthesized powder materials are in nanorange. The nanocomposite nanoparticles (NCsNPs) were synthesized via solution process with using copper nitrate hexahydrate, iron nitrate non hydrate as a main precursor material whereas sodium hydroxide was utilized as a salt reducing agent. The formed NCsNPs were characterized with X-ray diffraction pattern (XRD), Scanning electron microscopy (SEM), Transmission electron microscopy (TEM) and Fourier Transform infra-red spectroscopy (FTIR) were used for the structural and chemical characteristic in detail. It reveals that the NCNP is ∼45 nm in size with clear spherical shaped morphology with good chemical characteristics. The NCsNPs were utilized against cancer cells (C2C12 myoblast) for to reduce the growth rate with different incubation (24 & 48 h) periods. The % cells death was examined through MTT assay at different concentration (0.5 µg/mL, 1 µg/mL, 2 µg/mL, 5 µg/mL, 10 µg/mL, 25 µg/mL, 50 µg/mL, 100 µg/mL) of NCs with different (24 & 48 h) incubations respectively. The apoptosis in cells were analyzed with quantitative polymerase chain reaction (qPCR) study with apoptotic marker gens caspase 3 and 7 with control and the obtained data reveals that with the exposure of NCs, upregulated in gene expression at fixed concentration in 24 h incubation period. Based on acquired results a possible relation between the NCs and cancer cells were also discussed. |
| first_indexed | 2024-12-13T15:09:48Z |
| format | Article |
| id | doaj.art-eb7ef50c0b6e4c2da8f7ba40dfb00497 |
| institution | Directory Open Access Journal |
| issn | 1018-3647 |
| language | English |
| last_indexed | 2024-12-13T15:09:48Z |
| publishDate | 2022-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of King Saud University: Science |
| spelling | doaj.art-eb7ef50c0b6e4c2da8f7ba40dfb004972022-12-21T23:40:55ZengElsevierJournal of King Saud University: Science1018-36472022-04-01343101908Cytotoxic and molecular assessment with copper and iron nanocomposite, act as a soft eradicator against cancer cellsRizwan Wahab0Farheen Khan1Javed Ahmad2Abdulaziz A. Al-Khedhairy3Chair for DNA Research, College of Science, Department of Zoology, King Saud University, Riyadh 11451, Saudi Arabia; Zoology Department, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia; Corresponding authors at: Chair for DNA Research, College of Science, Department of Zoology, King Saud University, Riyadh 11451, Saudi Arabia (R. Wahab) and Chemistry Department, Faculty of Science, Taibah University, Madina (Yanbu), Saudi Arabia (F.Khan).Chemistry Department, Faculty of Science, Taibah University, Madina (Yanbu), Saudi Arabia; Corresponding authors at: Chair for DNA Research, College of Science, Department of Zoology, King Saud University, Riyadh 11451, Saudi Arabia (R. Wahab) and Chemistry Department, Faculty of Science, Taibah University, Madina (Yanbu), Saudi Arabia (F.Khan).Chair for DNA Research, College of Science, Department of Zoology, King Saud University, Riyadh 11451, Saudi ArabiaChair for DNA Research, College of Science, Department of Zoology, King Saud University, Riyadh 11451, Saudi Arabia; Zoology Department, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi ArabiaThe composites are the materials, which have either one or more combination with other material and exhibit extraordinary chemical characteristics. The present work describes the formation of composite of copper and iron metal oxide (CuFeO2). The synthesized powder materials are in nanorange. The nanocomposite nanoparticles (NCsNPs) were synthesized via solution process with using copper nitrate hexahydrate, iron nitrate non hydrate as a main precursor material whereas sodium hydroxide was utilized as a salt reducing agent. The formed NCsNPs were characterized with X-ray diffraction pattern (XRD), Scanning electron microscopy (SEM), Transmission electron microscopy (TEM) and Fourier Transform infra-red spectroscopy (FTIR) were used for the structural and chemical characteristic in detail. It reveals that the NCNP is ∼45 nm in size with clear spherical shaped morphology with good chemical characteristics. The NCsNPs were utilized against cancer cells (C2C12 myoblast) for to reduce the growth rate with different incubation (24 & 48 h) periods. The % cells death was examined through MTT assay at different concentration (0.5 µg/mL, 1 µg/mL, 2 µg/mL, 5 µg/mL, 10 µg/mL, 25 µg/mL, 50 µg/mL, 100 µg/mL) of NCs with different (24 & 48 h) incubations respectively. The apoptosis in cells were analyzed with quantitative polymerase chain reaction (qPCR) study with apoptotic marker gens caspase 3 and 7 with control and the obtained data reveals that with the exposure of NCs, upregulated in gene expression at fixed concentration in 24 h incubation period. Based on acquired results a possible relation between the NCs and cancer cells were also discussed.http://www.sciencedirect.com/science/article/pii/S1018364722000891NanocompositeSEMTEMMTT assayqPCR |
| spellingShingle | Rizwan Wahab Farheen Khan Javed Ahmad Abdulaziz A. Al-Khedhairy Cytotoxic and molecular assessment with copper and iron nanocomposite, act as a soft eradicator against cancer cells Journal of King Saud University: Science Nanocomposite SEM TEM MTT assay qPCR |
| title | Cytotoxic and molecular assessment with copper and iron nanocomposite, act as a soft eradicator against cancer cells |
| title_full | Cytotoxic and molecular assessment with copper and iron nanocomposite, act as a soft eradicator against cancer cells |
| title_fullStr | Cytotoxic and molecular assessment with copper and iron nanocomposite, act as a soft eradicator against cancer cells |
| title_full_unstemmed | Cytotoxic and molecular assessment with copper and iron nanocomposite, act as a soft eradicator against cancer cells |
| title_short | Cytotoxic and molecular assessment with copper and iron nanocomposite, act as a soft eradicator against cancer cells |
| title_sort | cytotoxic and molecular assessment with copper and iron nanocomposite act as a soft eradicator against cancer cells |
| topic | Nanocomposite SEM TEM MTT assay qPCR |
| url | http://www.sciencedirect.com/science/article/pii/S1018364722000891 |
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