OsDREB2A, a rice transcription factor, significantly affects salt tolerance in transgenic soybean.
The dehydration responsive element binding (DREB) transcription factors play an important role in regulating stress-related genes. OsDREB2A, a member of the DREBP subfamily of AP2/ERF transcription factors in rice (Oryza sativa), is involved in the abiotic stress response. OsDREB2A expression is ind...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3869746?pdf=render |
| _version_ | 1818430052460658688 |
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| author | Xiu-Xiang Zhang Yu-Juan Tang Qi-Bin Ma Cun-Yi Yang Ying-Hui Mu Hai-Cui Suo Lai-Hui Luo Hai Nian |
| author_facet | Xiu-Xiang Zhang Yu-Juan Tang Qi-Bin Ma Cun-Yi Yang Ying-Hui Mu Hai-Cui Suo Lai-Hui Luo Hai Nian |
| author_sort | Xiu-Xiang Zhang |
| collection | DOAJ |
| description | The dehydration responsive element binding (DREB) transcription factors play an important role in regulating stress-related genes. OsDREB2A, a member of the DREBP subfamily of AP2/ERF transcription factors in rice (Oryza sativa), is involved in the abiotic stress response. OsDREB2A expression is induced by drought, low-temperature and salt stresses. Here, we report the ability of OsDREB2A to regulate high-salt response in transgenic soybean. Overexpressing OsDREB2A in soybeans enhanced salt tolerance by accumulating osmolytes, such as soluble sugars and free proline, and improving the expression levels of some stress-responsive transcription factors and key genes. The phenotypic characterization of transgenic soybean were significantly better than those of wild-type (WT). Electrophoresis mobility shift assay (EMSA) revealed that the OsDREB2A can bind to the DRE core element in vitro. These results indicate that OsDREB2A may participate in abiotic stress by directly binding with DRE element to regulate the expression of downstream genes. Overexpression of OsDREB2A in soybean might be used to improve tolerance to salt stress. |
| first_indexed | 2024-12-14T15:27:17Z |
| format | Article |
| id | doaj.art-eb7f19f486bf494d915022890f27e185 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-14T15:27:17Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-eb7f19f486bf494d915022890f27e1852022-12-21T22:56:00ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-01812e8301110.1371/journal.pone.0083011OsDREB2A, a rice transcription factor, significantly affects salt tolerance in transgenic soybean.Xiu-Xiang ZhangYu-Juan TangQi-Bin MaCun-Yi YangYing-Hui MuHai-Cui SuoLai-Hui LuoHai NianThe dehydration responsive element binding (DREB) transcription factors play an important role in regulating stress-related genes. OsDREB2A, a member of the DREBP subfamily of AP2/ERF transcription factors in rice (Oryza sativa), is involved in the abiotic stress response. OsDREB2A expression is induced by drought, low-temperature and salt stresses. Here, we report the ability of OsDREB2A to regulate high-salt response in transgenic soybean. Overexpressing OsDREB2A in soybeans enhanced salt tolerance by accumulating osmolytes, such as soluble sugars and free proline, and improving the expression levels of some stress-responsive transcription factors and key genes. The phenotypic characterization of transgenic soybean were significantly better than those of wild-type (WT). Electrophoresis mobility shift assay (EMSA) revealed that the OsDREB2A can bind to the DRE core element in vitro. These results indicate that OsDREB2A may participate in abiotic stress by directly binding with DRE element to regulate the expression of downstream genes. Overexpression of OsDREB2A in soybean might be used to improve tolerance to salt stress.http://europepmc.org/articles/PMC3869746?pdf=render |
| spellingShingle | Xiu-Xiang Zhang Yu-Juan Tang Qi-Bin Ma Cun-Yi Yang Ying-Hui Mu Hai-Cui Suo Lai-Hui Luo Hai Nian OsDREB2A, a rice transcription factor, significantly affects salt tolerance in transgenic soybean. PLoS ONE |
| title | OsDREB2A, a rice transcription factor, significantly affects salt tolerance in transgenic soybean. |
| title_full | OsDREB2A, a rice transcription factor, significantly affects salt tolerance in transgenic soybean. |
| title_fullStr | OsDREB2A, a rice transcription factor, significantly affects salt tolerance in transgenic soybean. |
| title_full_unstemmed | OsDREB2A, a rice transcription factor, significantly affects salt tolerance in transgenic soybean. |
| title_short | OsDREB2A, a rice transcription factor, significantly affects salt tolerance in transgenic soybean. |
| title_sort | osdreb2a a rice transcription factor significantly affects salt tolerance in transgenic soybean |
| url | http://europepmc.org/articles/PMC3869746?pdf=render |
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