Broad dengue neutralization in mosquitoes expressing an engineered antibody.
With dengue virus (DENV) becoming endemic in tropical and subtropical regions worldwide, there is a pressing global demand for effective strategies to control the mosquitoes that spread this disease. Recent advances in genetic engineering technologies have made it possible to create mosquitoes with...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2020-01-01
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| Series: | PLoS Pathogens |
| Online Access: | https://doi.org/10.1371/journal.ppat.1008103 |
| _version_ | 1818926830386675712 |
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| author | Anna Buchman Stephanie Gamez Ming Li Igor Antoshechkin Hsing-Han Li Hsin-Wei Wang Chun-Hong Chen Melissa J Klein Jean-Bernard Duchemin James E Crowe Prasad N Paradkar Omar S Akbari |
| author_facet | Anna Buchman Stephanie Gamez Ming Li Igor Antoshechkin Hsing-Han Li Hsin-Wei Wang Chun-Hong Chen Melissa J Klein Jean-Bernard Duchemin James E Crowe Prasad N Paradkar Omar S Akbari |
| author_sort | Anna Buchman |
| collection | DOAJ |
| description | With dengue virus (DENV) becoming endemic in tropical and subtropical regions worldwide, there is a pressing global demand for effective strategies to control the mosquitoes that spread this disease. Recent advances in genetic engineering technologies have made it possible to create mosquitoes with reduced vector competence, limiting their ability to acquire and transmit pathogens. Here we describe the development of Aedes aegypti mosquitoes synthetically engineered to impede vector competence to DENV. These mosquitoes express a gene encoding an engineered single-chain variable fragment derived from a broadly neutralizing DENV human monoclonal antibody and have significantly reduced viral infection, dissemination, and transmission rates for all four major antigenically distinct DENV serotypes. Importantly, this is the first engineered approach that targets all DENV serotypes, which is crucial for effective disease suppression. These results provide a compelling route for developing effective genetic-based DENV control strategies, which could be extended to curtail other arboviruses. |
| first_indexed | 2024-12-20T03:03:21Z |
| format | Article |
| id | doaj.art-eb7f58b3071e483784b8e478e7845c30 |
| institution | Directory Open Access Journal |
| issn | 1553-7366 1553-7374 |
| language | English |
| last_indexed | 2024-12-20T03:03:21Z |
| publishDate | 2020-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Pathogens |
| spelling | doaj.art-eb7f58b3071e483784b8e478e7845c302022-12-21T19:55:40ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742020-01-01161e100810310.1371/journal.ppat.1008103Broad dengue neutralization in mosquitoes expressing an engineered antibody.Anna BuchmanStephanie GamezMing LiIgor AntoshechkinHsing-Han LiHsin-Wei WangChun-Hong ChenMelissa J KleinJean-Bernard DucheminJames E CrowePrasad N ParadkarOmar S AkbariWith dengue virus (DENV) becoming endemic in tropical and subtropical regions worldwide, there is a pressing global demand for effective strategies to control the mosquitoes that spread this disease. Recent advances in genetic engineering technologies have made it possible to create mosquitoes with reduced vector competence, limiting their ability to acquire and transmit pathogens. Here we describe the development of Aedes aegypti mosquitoes synthetically engineered to impede vector competence to DENV. These mosquitoes express a gene encoding an engineered single-chain variable fragment derived from a broadly neutralizing DENV human monoclonal antibody and have significantly reduced viral infection, dissemination, and transmission rates for all four major antigenically distinct DENV serotypes. Importantly, this is the first engineered approach that targets all DENV serotypes, which is crucial for effective disease suppression. These results provide a compelling route for developing effective genetic-based DENV control strategies, which could be extended to curtail other arboviruses.https://doi.org/10.1371/journal.ppat.1008103 |
| spellingShingle | Anna Buchman Stephanie Gamez Ming Li Igor Antoshechkin Hsing-Han Li Hsin-Wei Wang Chun-Hong Chen Melissa J Klein Jean-Bernard Duchemin James E Crowe Prasad N Paradkar Omar S Akbari Broad dengue neutralization in mosquitoes expressing an engineered antibody. PLoS Pathogens |
| title | Broad dengue neutralization in mosquitoes expressing an engineered antibody. |
| title_full | Broad dengue neutralization in mosquitoes expressing an engineered antibody. |
| title_fullStr | Broad dengue neutralization in mosquitoes expressing an engineered antibody. |
| title_full_unstemmed | Broad dengue neutralization in mosquitoes expressing an engineered antibody. |
| title_short | Broad dengue neutralization in mosquitoes expressing an engineered antibody. |
| title_sort | broad dengue neutralization in mosquitoes expressing an engineered antibody |
| url | https://doi.org/10.1371/journal.ppat.1008103 |
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