A multigene family encoding surface glycoproteins in Trypanosoma congolense
Trypanosoma congolense, the causative agent of the most important livestock disease in Africa, expresses specific surface proteins involved in its parasitic lifestyle. Unfortunately, the complete repertoire of such molecules is far from being deciphered. As these membrane components are exposed to t...
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| Format: | Article |
| Language: | English |
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Shared Science Publishers OG
2017-03-01
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| Series: | Microbial Cell |
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| Online Access: | http://microbialcell.com/researcharticles/a-multigene-family-encoding-surface-glycoproteins-in-trypanosoma-congolense/ |
| _version_ | 1826532069109399552 |
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| author | Magali Thonnus Amandine Guéri Loïc Rivière |
| author_facet | Magali Thonnus Amandine Guéri Loïc Rivière |
| author_sort | Magali Thonnus |
| collection | DOAJ |
| description | Trypanosoma congolense, the causative agent of the most important livestock disease in Africa, expresses specific surface proteins involved in its parasitic lifestyle. Unfortunately, the complete repertoire of such molecules is far from being deciphered. As these membrane components are exposed to the host environment, they could be used as therapeutic or diagnostic targets. By mining the T. congolense genome database, we identified a novel family of lectin-like glycoproteins (TcoClecs). These molecules are predicted to have a transmembrane domain, a tandem repeat amino acid motif, a signal peptide and a C-type lectin-like domain (CTLD). This paper depicts several experimental arguments in favor of a surface localization in bloodstream forms of T. congolense. A TcoClec gene was heterologously expressed in U-2 OS cells and the product could be partially found at the plasma membrane. TcoClecs were also localized at the surface of T. congolense bloodstream forms. The signal was suppressed when the cells were treated with a detergent to remove the plasma membrane or with trypsin to « shave » the parasites and remove their external proteins. This suggests that TcoClecs could be potential diagnostic or therapeutic antigens of African animal trypanosomiasis. The potential role of these proteins in T. congolense as well as in other trypanosomatids is discussed. |
| first_indexed | 2024-04-12T11:42:23Z |
| format | Article |
| id | doaj.art-eb7f93ab684246dab2f116bcc59c5123 |
| institution | Directory Open Access Journal |
| issn | 2311-2638 |
| language | English |
| last_indexed | 2025-03-14T01:45:22Z |
| publishDate | 2017-03-01 |
| publisher | Shared Science Publishers OG |
| record_format | Article |
| series | Microbial Cell |
| spelling | doaj.art-eb7f93ab684246dab2f116bcc59c51232025-03-12T13:46:42ZengShared Science Publishers OGMicrobial Cell2311-26382017-03-0143909710.15698/mic2017.02.562A multigene family encoding surface glycoproteins in Trypanosoma congolenseMagali Thonnus0Amandine Guéri1Loïc Rivière2Fundamental Microbiology and Pathogenicity Unit, CNRS UMR 5234, Bordeaux University, France.Fundamental Microbiology and Pathogenicity Unit, CNRS UMR 5234, Bordeaux University, France.Fundamental Microbiology and Pathogenicity Unit, CNRS UMR 5234, Bordeaux University, France.Trypanosoma congolense, the causative agent of the most important livestock disease in Africa, expresses specific surface proteins involved in its parasitic lifestyle. Unfortunately, the complete repertoire of such molecules is far from being deciphered. As these membrane components are exposed to the host environment, they could be used as therapeutic or diagnostic targets. By mining the T. congolense genome database, we identified a novel family of lectin-like glycoproteins (TcoClecs). These molecules are predicted to have a transmembrane domain, a tandem repeat amino acid motif, a signal peptide and a C-type lectin-like domain (CTLD). This paper depicts several experimental arguments in favor of a surface localization in bloodstream forms of T. congolense. A TcoClec gene was heterologously expressed in U-2 OS cells and the product could be partially found at the plasma membrane. TcoClecs were also localized at the surface of T. congolense bloodstream forms. The signal was suppressed when the cells were treated with a detergent to remove the plasma membrane or with trypsin to « shave » the parasites and remove their external proteins. This suggests that TcoClecs could be potential diagnostic or therapeutic antigens of African animal trypanosomiasis. The potential role of these proteins in T. congolense as well as in other trypanosomatids is discussed.http://microbialcell.com/researcharticles/a-multigene-family-encoding-surface-glycoproteins-in-trypanosoma-congolense/Trypanosoma congolensesurface glycoproteinmultigene familylectin-liketrypanosomes |
| spellingShingle | Magali Thonnus Amandine Guéri Loïc Rivière A multigene family encoding surface glycoproteins in Trypanosoma congolense Microbial Cell Trypanosoma congolense surface glycoprotein multigene family lectin-like trypanosomes |
| title | A multigene family encoding surface glycoproteins in Trypanosoma congolense |
| title_full | A multigene family encoding surface glycoproteins in Trypanosoma congolense |
| title_fullStr | A multigene family encoding surface glycoproteins in Trypanosoma congolense |
| title_full_unstemmed | A multigene family encoding surface glycoproteins in Trypanosoma congolense |
| title_short | A multigene family encoding surface glycoproteins in Trypanosoma congolense |
| title_sort | multigene family encoding surface glycoproteins in trypanosoma congolense |
| topic | Trypanosoma congolense surface glycoprotein multigene family lectin-like trypanosomes |
| url | http://microbialcell.com/researcharticles/a-multigene-family-encoding-surface-glycoproteins-in-trypanosoma-congolense/ |
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