Approaches to gene pool conservation of medicinal plant Oxytropis lanata (Pall.) DC. (Fabaceae)

In order to preserve the gene pool of medicinal plant Oxytropis lanata (Pall.) DC. we analyzed allozyme polymorphism and identified reliable and informative marker enzyme systems of this species; also we studied the response of seeds to deep freezing in liquid nitrogen (–196 ºС). Population has an a...

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Main Authors: A. B. Kholina, N. M. Voronkova, O. V. Nakonechaya, O. G. Koren’
Format: Article
Language:English
Published: Altay State University 2015-05-01
Series:Turczaninowia
Online Access:http://journal.asu.ru/index.php/tur/article/view/748
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author A. B. Kholina
N. M. Voronkova
O. V. Nakonechaya
O. G. Koren’
author_facet A. B. Kholina
N. M. Voronkova
O. V. Nakonechaya
O. G. Koren’
author_sort A. B. Kholina
collection DOAJ
description In order to preserve the gene pool of medicinal plant Oxytropis lanata (Pall.) DC. we analyzed allozyme polymorphism and identified reliable and informative marker enzyme systems of this species; also we studied the response of seeds to deep freezing in liquid nitrogen (–196 ºС). Population has an average level of polymorphism (P95 = 41,2 %, P99 = 52,9 %, A = 1,58, Ho = 0,158, He = 0,171) in general typical for herbaceous legumes, and can serve as a source of material for gene pool conservation of the species. Deep freezing has not led to the death of the seeds; it was marked stimulatory effect of ultralow temperatures, expressed as an acceleration of germination and sharp increase of germinability (98,6 ± 2,3 %) compared to the control (12,0 ± 3,5 %) that is associated with overcoming physical dormancy. There were no abnormalities in the development of seedlings from seeds passed cryopreservation.
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spelling doaj.art-eb82182b68fa4b7ab9c8ea54ba5f301e2022-12-21T18:23:00ZengAltay State UniversityTurczaninowia1560-72591560-72672015-05-0118110.14258/turczaninowia.18.1.11518Approaches to gene pool conservation of medicinal plant Oxytropis lanata (Pall.) DC. (Fabaceae)A. B. Kholina0N. M. Voronkova1O. V. Nakonechaya2O. G. Koren’3Institute of Biology and Soil Science FEB RAS, Vladivostok, RussiaInstitute of Biology and Soil Science FEB RAS, Vladivostok, RussiaInstitute of Biology and Soil Science FEB RAS, Vladivostok, RussiaInstitute of Biology and Soil Science FEB RAS, Vladivostok, RussiaIn order to preserve the gene pool of medicinal plant Oxytropis lanata (Pall.) DC. we analyzed allozyme polymorphism and identified reliable and informative marker enzyme systems of this species; also we studied the response of seeds to deep freezing in liquid nitrogen (–196 ºС). Population has an average level of polymorphism (P95 = 41,2 %, P99 = 52,9 %, A = 1,58, Ho = 0,158, He = 0,171) in general typical for herbaceous legumes, and can serve as a source of material for gene pool conservation of the species. Deep freezing has not led to the death of the seeds; it was marked stimulatory effect of ultralow temperatures, expressed as an acceleration of germination and sharp increase of germinability (98,6 ± 2,3 %) compared to the control (12,0 ± 3,5 %) that is associated with overcoming physical dormancy. There were no abnormalities in the development of seedlings from seeds passed cryopreservation.http://journal.asu.ru/index.php/tur/article/view/748
spellingShingle A. B. Kholina
N. M. Voronkova
O. V. Nakonechaya
O. G. Koren’
Approaches to gene pool conservation of medicinal plant Oxytropis lanata (Pall.) DC. (Fabaceae)
Turczaninowia
title Approaches to gene pool conservation of medicinal plant Oxytropis lanata (Pall.) DC. (Fabaceae)
title_full Approaches to gene pool conservation of medicinal plant Oxytropis lanata (Pall.) DC. (Fabaceae)
title_fullStr Approaches to gene pool conservation of medicinal plant Oxytropis lanata (Pall.) DC. (Fabaceae)
title_full_unstemmed Approaches to gene pool conservation of medicinal plant Oxytropis lanata (Pall.) DC. (Fabaceae)
title_short Approaches to gene pool conservation of medicinal plant Oxytropis lanata (Pall.) DC. (Fabaceae)
title_sort approaches to gene pool conservation of medicinal plant oxytropis lanata pall dc fabaceae
url http://journal.asu.ru/index.php/tur/article/view/748
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AT ovnakonechaya approachestogenepoolconservationofmedicinalplantoxytropislanatapalldcfabaceae
AT ogkoren approachestogenepoolconservationofmedicinalplantoxytropislanatapalldcfabaceae