| Summary: | The fate of intraperitoneally injected or implanted male rat bone
marrow-derived stromal cells inside female sibling host animals was traced using
Y-chromosome-sensitive PCR. When injected intraperitoneally, Y-chromosome-positive cells
were found in all studied organs: heart muscle, lung, thymus, liver, spleen, kidney,
skin, and femoral bone marrow with a few exceptions regardless of whether they had gone
through osteogenic differentiation or not. In the implant experiments, expanded donor
cells were seeded on poly(lactide-co-glycolide) scaffolds and grown under three
different conditions (no additives, in osteogenic media for one or two weeks) prior to
implantation into corticomedullar femoral defects. Although the impact of osteogenic in
vitro cell differentiation on cell migration was more obvious in the implantation
experiments than in the intraperitoneal experiments, the donor cells stay alive when
injected intraperitoneally or grown in an implant and migrate inside the host. However,
when the implants contained bioactive glass, no signs of Y-chromosomal DNA were observed
in all studied organs including the implants indicating that the cells had been
eliminated.
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