Fate of Bone Marrow-Derived Stromal Cells after Intraperitoneal Infusion or Implantation into Femoral Bone Defects in the Host Animal
The fate of intraperitoneally injected or implanted male rat bone marrow-derived stromal cells inside female sibling host animals was traced using Y-chromosome-sensitive PCR. When injected intraperitoneally, Y-chromosome-positive cells were found in all studied or...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2010-01-01
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| Series: | Journal of Tissue Engineering |
| Online Access: | http://tej.sagepub.com/content/1/1/10.4061_2010/345806.full.pdf |
| _version_ | 1818146596404068352 |
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| author | Timothy Wilson Christoffer Stark Johanna Holmbom Ari Rosling Asko Kuusilehto Teemu Tirri Risto Penttinen Erika Ekholm |
| author_facet | Timothy Wilson Christoffer Stark Johanna Holmbom Ari Rosling Asko Kuusilehto Teemu Tirri Risto Penttinen Erika Ekholm |
| author_sort | Timothy Wilson |
| collection | DOAJ |
| description | The fate of intraperitoneally injected or implanted male rat bone
marrow-derived stromal cells inside female sibling host animals was traced using
Y-chromosome-sensitive PCR. When injected intraperitoneally, Y-chromosome-positive cells
were found in all studied organs: heart muscle, lung, thymus, liver, spleen, kidney,
skin, and femoral bone marrow with a few exceptions regardless of whether they had gone
through osteogenic differentiation or not. In the implant experiments, expanded donor
cells were seeded on poly(lactide-co-glycolide) scaffolds and grown under three
different conditions (no additives, in osteogenic media for one or two weeks) prior to
implantation into corticomedullar femoral defects. Although the impact of osteogenic in
vitro cell differentiation on cell migration was more obvious in the implantation
experiments than in the intraperitoneal experiments, the donor cells stay alive when
injected intraperitoneally or grown in an implant and migrate inside the host. However,
when the implants contained bioactive glass, no signs of Y-chromosomal DNA were observed
in all studied organs including the implants indicating that the cells had been
eliminated. |
| first_indexed | 2024-12-11T12:21:52Z |
| format | Article |
| id | doaj.art-eb85247413c94e2eb9dce4eb75637396 |
| institution | Directory Open Access Journal |
| issn | 2041-7314 |
| language | English |
| last_indexed | 2024-12-11T12:21:52Z |
| publishDate | 2010-01-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Journal of Tissue Engineering |
| spelling | doaj.art-eb85247413c94e2eb9dce4eb756373962022-12-22T01:07:30ZengSAGE PublishingJournal of Tissue Engineering2041-73142010-01-011110.4061/2010/34580610.4061_2010/345806Fate of Bone Marrow-Derived Stromal Cells after Intraperitoneal Infusion or Implantation into Femoral Bone Defects in the Host AnimalTimothy WilsonChristoffer StarkJohanna HolmbomAri RoslingAsko KuusilehtoTeemu TirriRisto PenttinenErika EkholmThe fate of intraperitoneally injected or implanted male rat bone marrow-derived stromal cells inside female sibling host animals was traced using Y-chromosome-sensitive PCR. When injected intraperitoneally, Y-chromosome-positive cells were found in all studied organs: heart muscle, lung, thymus, liver, spleen, kidney, skin, and femoral bone marrow with a few exceptions regardless of whether they had gone through osteogenic differentiation or not. In the implant experiments, expanded donor cells were seeded on poly(lactide-co-glycolide) scaffolds and grown under three different conditions (no additives, in osteogenic media for one or two weeks) prior to implantation into corticomedullar femoral defects. Although the impact of osteogenic in vitro cell differentiation on cell migration was more obvious in the implantation experiments than in the intraperitoneal experiments, the donor cells stay alive when injected intraperitoneally or grown in an implant and migrate inside the host. However, when the implants contained bioactive glass, no signs of Y-chromosomal DNA were observed in all studied organs including the implants indicating that the cells had been eliminated.http://tej.sagepub.com/content/1/1/10.4061_2010/345806.full.pdf |
| spellingShingle | Timothy Wilson Christoffer Stark Johanna Holmbom Ari Rosling Asko Kuusilehto Teemu Tirri Risto Penttinen Erika Ekholm Fate of Bone Marrow-Derived Stromal Cells after Intraperitoneal Infusion or Implantation into Femoral Bone Defects in the Host Animal Journal of Tissue Engineering |
| title | Fate of Bone Marrow-Derived Stromal Cells after Intraperitoneal
Infusion or Implantation into Femoral Bone Defects in the Host Animal |
| title_full | Fate of Bone Marrow-Derived Stromal Cells after Intraperitoneal
Infusion or Implantation into Femoral Bone Defects in the Host Animal |
| title_fullStr | Fate of Bone Marrow-Derived Stromal Cells after Intraperitoneal
Infusion or Implantation into Femoral Bone Defects in the Host Animal |
| title_full_unstemmed | Fate of Bone Marrow-Derived Stromal Cells after Intraperitoneal
Infusion or Implantation into Femoral Bone Defects in the Host Animal |
| title_short | Fate of Bone Marrow-Derived Stromal Cells after Intraperitoneal
Infusion or Implantation into Femoral Bone Defects in the Host Animal |
| title_sort | fate of bone marrow derived stromal cells after intraperitoneal infusion or implantation into femoral bone defects in the host animal |
| url | http://tej.sagepub.com/content/1/1/10.4061_2010/345806.full.pdf |
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