Effects of Caffeine on Metabolism and Mitochondria Biogenesis in Rhabdomyosarcoma Cells Compared with 2,4-Dinitrophenol
Purpose This work investigated if treatment with caffeine or 2,4-dinitrophenol (DNP) induce expression of peroxisome proliferator-activated receptor coactivator 1 alpha (PGC-1α) and increase both mitochondrial biosynthesis and metabolism in skeletal muscle. Methods Human rhabdomyosarcoma cells were...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2012-01-01
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| Series: | Nutrition and Metabolic Insights |
| Online Access: | https://doi.org/10.4137/NMI.S10233 |
| _version_ | 1831601719591567360 |
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| author | Roger A. Vaughan Randi Garcia-Smith Marco Bisoffi Kristina A. Trujillo Carole A. Conn |
| author_facet | Roger A. Vaughan Randi Garcia-Smith Marco Bisoffi Kristina A. Trujillo Carole A. Conn |
| author_sort | Roger A. Vaughan |
| collection | DOAJ |
| description | Purpose This work investigated if treatment with caffeine or 2,4-dinitrophenol (DNP) induce expression of peroxisome proliferator-activated receptor coactivator 1 alpha (PGC-1α) and increase both mitochondrial biosynthesis and metabolism in skeletal muscle. Methods Human rhabdomyosarcoma cells were treated with either ethanol control (0.1% final concentration) caffeine, or DNP at 250 or 500 μM for 16 or 24 hours. PGC-1α RNA levels were determined using quantitative reverse transcriptase polymerase chain reaction (qRT-PCR). PGC-1α protein and mitochondrial content was determined using flow cytometry and immunohistochemistry. Metabolism was determined by quantification of extracellular acidification rate and oxygen consumption rate. Results Treatment with either caffeine or DNP induced PGC-1α RNA and protein as well as mitochondrial content compared with control. Treatment with caffeine and DNP also significantly increased oxidative metabolism and total metabolic rate compared with control. Caffeine similarly increased metabolism and mitochondrial content compared with DNP. Conclusion This work identified that both caffeine and DNP significantly induce PGC-1α, and increase both metabolism and mitochondrial content in skeletal muscle. |
| first_indexed | 2024-12-18T15:08:28Z |
| format | Article |
| id | doaj.art-eb85d1298d904c558ece04cf1dee49d5 |
| institution | Directory Open Access Journal |
| issn | 1178-6388 |
| language | English |
| last_indexed | 2024-12-18T15:08:28Z |
| publishDate | 2012-01-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Nutrition and Metabolic Insights |
| spelling | doaj.art-eb85d1298d904c558ece04cf1dee49d52022-12-21T21:03:44ZengSAGE PublishingNutrition and Metabolic Insights1178-63882012-01-01510.4137/NMI.S10233Effects of Caffeine on Metabolism and Mitochondria Biogenesis in Rhabdomyosarcoma Cells Compared with 2,4-DinitrophenolRoger A. Vaughan0Randi Garcia-Smith1Marco Bisoffi2Kristina A. Trujillo3Carole A. Conn4Department of IFCE: Nutrition, University of New Mexico, Albuquerque.Department of Biochemistry and Molecular Biology, University of New Mexico Health Sciences Center, Albuquerque.Department of Biochemistry and Molecular Biology, University of New Mexico Health Sciences Center, Albuquerque.Department of Biochemistry and Molecular Biology, University of New Mexico Health Sciences Center, Albuquerque.Department of IFCE: Nutrition, University of New Mexico, Albuquerque.Purpose This work investigated if treatment with caffeine or 2,4-dinitrophenol (DNP) induce expression of peroxisome proliferator-activated receptor coactivator 1 alpha (PGC-1α) and increase both mitochondrial biosynthesis and metabolism in skeletal muscle. Methods Human rhabdomyosarcoma cells were treated with either ethanol control (0.1% final concentration) caffeine, or DNP at 250 or 500 μM for 16 or 24 hours. PGC-1α RNA levels were determined using quantitative reverse transcriptase polymerase chain reaction (qRT-PCR). PGC-1α protein and mitochondrial content was determined using flow cytometry and immunohistochemistry. Metabolism was determined by quantification of extracellular acidification rate and oxygen consumption rate. Results Treatment with either caffeine or DNP induced PGC-1α RNA and protein as well as mitochondrial content compared with control. Treatment with caffeine and DNP also significantly increased oxidative metabolism and total metabolic rate compared with control. Caffeine similarly increased metabolism and mitochondrial content compared with DNP. Conclusion This work identified that both caffeine and DNP significantly induce PGC-1α, and increase both metabolism and mitochondrial content in skeletal muscle.https://doi.org/10.4137/NMI.S10233 |
| spellingShingle | Roger A. Vaughan Randi Garcia-Smith Marco Bisoffi Kristina A. Trujillo Carole A. Conn Effects of Caffeine on Metabolism and Mitochondria Biogenesis in Rhabdomyosarcoma Cells Compared with 2,4-Dinitrophenol Nutrition and Metabolic Insights |
| title | Effects of Caffeine on Metabolism and Mitochondria Biogenesis in Rhabdomyosarcoma Cells Compared with 2,4-Dinitrophenol |
| title_full | Effects of Caffeine on Metabolism and Mitochondria Biogenesis in Rhabdomyosarcoma Cells Compared with 2,4-Dinitrophenol |
| title_fullStr | Effects of Caffeine on Metabolism and Mitochondria Biogenesis in Rhabdomyosarcoma Cells Compared with 2,4-Dinitrophenol |
| title_full_unstemmed | Effects of Caffeine on Metabolism and Mitochondria Biogenesis in Rhabdomyosarcoma Cells Compared with 2,4-Dinitrophenol |
| title_short | Effects of Caffeine on Metabolism and Mitochondria Biogenesis in Rhabdomyosarcoma Cells Compared with 2,4-Dinitrophenol |
| title_sort | effects of caffeine on metabolism and mitochondria biogenesis in rhabdomyosarcoma cells compared with 2 4 dinitrophenol |
| url | https://doi.org/10.4137/NMI.S10233 |
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