Fluoride-induced apoptosis in non-skeletal tissues of experimental animals: A systematic review and meta-analysis
Different studies have suggested that fluoride can induce apoptosis in non-skeletal tissues, however, evidence from these experimental studies is still controversial. This meta-analysis aims to clarify the mechanism of fluoride-induced apoptosis in non-skeletal tissues of experimental animals. Prima...
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Elsevier
2023-08-01
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844023058541 |
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| author | Linet Musungu Angwa Sylvester Dodzi Nyadanu Anne Murugi Kanyugo Timothy Adampah Gavin Pereira |
| author_facet | Linet Musungu Angwa Sylvester Dodzi Nyadanu Anne Murugi Kanyugo Timothy Adampah Gavin Pereira |
| author_sort | Linet Musungu Angwa |
| collection | DOAJ |
| description | Different studies have suggested that fluoride can induce apoptosis in non-skeletal tissues, however, evidence from these experimental studies is still controversial. This meta-analysis aims to clarify the mechanism of fluoride-induced apoptosis in non-skeletal tissues of experimental animals. Primary studies which measured apoptosis were identified through exhaustive database searching in PubMed, Embase, Web of Science Core Collection, Scopus, and references of included studies. A random effects model with standardized mean difference (SMD) was used for meta-analyses. The heterogeneity of the studies was evaluated using Higgin's I2 statistics. The risk of bias and publication bias were assessed using the SYRCLE's risk of bias tool and Egger's test, respectively. There was an increase in total apoptotic cells, and the expression of Bax, Bax/Bcl-2 ratio, caspase-3, caspase-8, caspase-9, Cyt c, and p53, and a decrease in the expression of Bcl-2 in the fluoride-treated groups as compared to the control groups. However, there was no evidence of a difference in the expression of APAF-1 in the two groups. The subgroup analysis highlighted the role of the intervention period in modification of the apoptotic effect of fluoride and that the susceptibility and tolerance of different animal species and tissues vary. Meta-regression analysis indicated that the studies' effect size for total apoptotic cells was influenced by animal species and that of Bax by the sample source. The results of this meta-analysis revealed that fluoride causes apoptosis by up-regulating caspase-3, -8, and -9, Cyt c, p53, Bax, and down-regulating Bcl-2 with a concomitant up-regulation of the Bax/Bcl-2 ratio. |
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| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-12T12:22:09Z |
| publishDate | 2023-08-01 |
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| spelling | doaj.art-eb869b2fcfdb4380a66f394397f5ca9a2023-08-30T05:52:04ZengElsevierHeliyon2405-84402023-08-0198e18646Fluoride-induced apoptosis in non-skeletal tissues of experimental animals: A systematic review and meta-analysisLinet Musungu Angwa0Sylvester Dodzi Nyadanu1Anne Murugi Kanyugo2Timothy Adampah3Gavin Pereira4Department of Clinical Medicine, Kabarak University, Private Bag, 20157, Kabarak, Kenya; Corresponding author.Curtin School of Population Health, Curtin University, Perth, Kent Street, Bentley, Western Australia, 6102, Australia; Education, Culture, and Health Opportunities (ECHO) Research Group International, Aflao, GhanaDepartment of Clinical Medicine, Kabarak University, Private Bag, 20157, Kabarak, KenyaEducation, Culture, and Health Opportunities (ECHO) Research Group International, Aflao, GhanaCurtin School of Population Health, Curtin University, Perth, Kent Street, Bentley, Western Australia, 6102, Australia; Centre for Fertility and Health (CeFH), Norwegian Institute of Public Health, 0473, Oslo, Norway; enAble Institute, Curtin University, Perth, Kent Street, Bentley, Western Australia, 6102, AustraliaDifferent studies have suggested that fluoride can induce apoptosis in non-skeletal tissues, however, evidence from these experimental studies is still controversial. This meta-analysis aims to clarify the mechanism of fluoride-induced apoptosis in non-skeletal tissues of experimental animals. Primary studies which measured apoptosis were identified through exhaustive database searching in PubMed, Embase, Web of Science Core Collection, Scopus, and references of included studies. A random effects model with standardized mean difference (SMD) was used for meta-analyses. The heterogeneity of the studies was evaluated using Higgin's I2 statistics. The risk of bias and publication bias were assessed using the SYRCLE's risk of bias tool and Egger's test, respectively. There was an increase in total apoptotic cells, and the expression of Bax, Bax/Bcl-2 ratio, caspase-3, caspase-8, caspase-9, Cyt c, and p53, and a decrease in the expression of Bcl-2 in the fluoride-treated groups as compared to the control groups. However, there was no evidence of a difference in the expression of APAF-1 in the two groups. The subgroup analysis highlighted the role of the intervention period in modification of the apoptotic effect of fluoride and that the susceptibility and tolerance of different animal species and tissues vary. Meta-regression analysis indicated that the studies' effect size for total apoptotic cells was influenced by animal species and that of Bax by the sample source. The results of this meta-analysis revealed that fluoride causes apoptosis by up-regulating caspase-3, -8, and -9, Cyt c, p53, Bax, and down-regulating Bcl-2 with a concomitant up-regulation of the Bax/Bcl-2 ratio.http://www.sciencedirect.com/science/article/pii/S2405844023058541ApoptosisFluorosisMeta-analysisSystematic review |
| spellingShingle | Linet Musungu Angwa Sylvester Dodzi Nyadanu Anne Murugi Kanyugo Timothy Adampah Gavin Pereira Fluoride-induced apoptosis in non-skeletal tissues of experimental animals: A systematic review and meta-analysis Heliyon Apoptosis Fluorosis Meta-analysis Systematic review |
| title | Fluoride-induced apoptosis in non-skeletal tissues of experimental animals: A systematic review and meta-analysis |
| title_full | Fluoride-induced apoptosis in non-skeletal tissues of experimental animals: A systematic review and meta-analysis |
| title_fullStr | Fluoride-induced apoptosis in non-skeletal tissues of experimental animals: A systematic review and meta-analysis |
| title_full_unstemmed | Fluoride-induced apoptosis in non-skeletal tissues of experimental animals: A systematic review and meta-analysis |
| title_short | Fluoride-induced apoptosis in non-skeletal tissues of experimental animals: A systematic review and meta-analysis |
| title_sort | fluoride induced apoptosis in non skeletal tissues of experimental animals a systematic review and meta analysis |
| topic | Apoptosis Fluorosis Meta-analysis Systematic review |
| url | http://www.sciencedirect.com/science/article/pii/S2405844023058541 |
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