Molecular epidemiology of enterically colonizing Escherichia coli with resistance against third-generation cephalosporins isolated from stool samples of European soldiers with concomitant diarrhea on deployment in Western African Mali
Extended spectrum beta-lactamases (ESBL) are frequently found in Enterobacterales isolates from Western Africa. However, information on the molecular epidemiology of regional ESBL-positive Enterobacterales strains is scarce. In order to provide epidemiological information, ESBL-positive Escherichia...
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Frontiers Media S.A.
2023-05-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1169829/full |
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| author | Katharina Hoffmann Matthias Riediger Aljoscha Tersteegen Pauline Marquardt Sascha Kahlfuß Sascha Kahlfuß Sascha Kahlfuß Sascha Kahlfuß Achim J. Kaasch Ralf Matthias Hagen Hagen Frickmann Hagen Frickmann Andreas E. Zautner Andreas E. Zautner |
| author_facet | Katharina Hoffmann Matthias Riediger Aljoscha Tersteegen Pauline Marquardt Sascha Kahlfuß Sascha Kahlfuß Sascha Kahlfuß Sascha Kahlfuß Achim J. Kaasch Ralf Matthias Hagen Hagen Frickmann Hagen Frickmann Andreas E. Zautner Andreas E. Zautner |
| author_sort | Katharina Hoffmann |
| collection | DOAJ |
| description | Extended spectrum beta-lactamases (ESBL) are frequently found in Enterobacterales isolates from Western Africa. However, information on the molecular epidemiology of regional ESBL-positive Enterobacterales strains is scarce. In order to provide epidemiological information, ESBL-positive Escherichia coli isolates from stool samples of European soldiers with diarrhea deployed to a field camp in Mali were subjected to whole-genome sequencing (Illumina MiSeq and Oxford Nanopore MinION) and antimicrobial susceptibility testing. With two exemptions, sequence-based analysis suggested an absence of transmission events between soldiers as indicated by a high genetic diversity of isolates and sequence types, confirming previous rep-PCR results. Third-generation cephalosporin resistance was associated with the presence of blaCTX-M-15 genes with (n = 14) and without (n = 5) co-occurring blaTEM-1b genes. Between 0 and 6 virulence and resistance plasmids per isolate were recorded. The detected resistance plasmids could be categorized into five types, which, in turn, share different sequence-identical segments, representing particular antimicrobial resistance gene-associated mobile genetic elements (MGEs). Phenotypic resistance rates within the 19 assessed isolates that showed distinguishable colony morphologies were 94.7% (18/19) against ampicillin-sulbactam and trimethoprim/sulfamethoxazole, 68.4% (13/19) against moxifloxacin, 31.6% (6/19) against ciprofloxacin, 42.1% (8/19) against gentamicin, 31.6% (6/19) against tobramycin, and 21.1% (4/19) against piperacillin-tazobactam and fosfomycin. Virulence-associated genes mediating infectious gastroenteritis were rarely detected. The gene aggR, which is characteristic for enteroaggregative E. coli, was only detected in one single isolate. In summary, we found a variety of different strains and clonal lineages of ESBL-carrying E. coli. Transmission either between soldiers or from common contaminated sources was demonstrated in two cases and played only a minor role in this military field camp, while there were indications that resistance gene bearing MGEs had been exchanged between antimicrobial resistance gene-(ARG-)carrying plasmids. |
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| spelling | doaj.art-eb87b5024a2f4520a18fc3630bd171f32023-05-05T05:49:43ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-05-011410.3389/fmicb.2023.11698291169829Molecular epidemiology of enterically colonizing Escherichia coli with resistance against third-generation cephalosporins isolated from stool samples of European soldiers with concomitant diarrhea on deployment in Western African MaliKatharina Hoffmann0Matthias Riediger1Aljoscha Tersteegen2Pauline Marquardt3Sascha Kahlfuß4Sascha Kahlfuß5Sascha Kahlfuß6Sascha Kahlfuß7Achim J. Kaasch8Ralf Matthias Hagen9Hagen Frickmann10Hagen Frickmann11Andreas E. Zautner12Andreas E. Zautner13Institute of Medical Microbiology and Hospital Hygiene, Medical Faculty, Otto-von-Guericke University Magdeburg, Magdeburg, GermanyInstitute of Medical Microbiology and Hospital Hygiene, Medical Faculty, Otto-von-Guericke University Magdeburg, Magdeburg, GermanyInstitute of Medical Microbiology and Hospital Hygiene, Medical Faculty, Otto-von-Guericke University Magdeburg, Magdeburg, GermanyInstitute of Medical Microbiology and Hospital Hygiene, Medical Faculty, Otto-von-Guericke University Magdeburg, Magdeburg, GermanyInstitute of Medical Microbiology and Hospital Hygiene, Medical Faculty, Otto-von-Guericke University Magdeburg, Magdeburg, GermanyInstitute of Molecular and Clinical Immunology, Medical Faculty, Otto-von-Guericke University Magdeburg, Magdeburg, GermanyHealth Campus Immunology, Infectiology, and Inflammation (GCI3), Medical Center, Otto-von-Guericke University Magdeburg, Magdeburg, GermanyCHaMP, Center for Health and Medical Prevention, Otto-von-Guericke-University Magdeburg, Magdeburg, GermanyInstitute of Medical Microbiology and Hospital Hygiene, Medical Faculty, Otto-von-Guericke University Magdeburg, Magdeburg, GermanyDepartment of Microbiology and Hospital Hygiene, Bundeswehr Central Hospital Koblenz, Koblenz, GermanyDepartment of Microbiology and Hospital Hygiene, Bundeswehr Hospital Hamburg, Hamburg, GermanyInstitute for Medical Microbiology, Virology and Hospital Hygiene, University Medicine Rostock, Rostock, GermanyInstitute of Medical Microbiology and Hospital Hygiene, Medical Faculty, Otto-von-Guericke University Magdeburg, Magdeburg, GermanyCHaMP, Center for Health and Medical Prevention, Otto-von-Guericke-University Magdeburg, Magdeburg, GermanyExtended spectrum beta-lactamases (ESBL) are frequently found in Enterobacterales isolates from Western Africa. However, information on the molecular epidemiology of regional ESBL-positive Enterobacterales strains is scarce. In order to provide epidemiological information, ESBL-positive Escherichia coli isolates from stool samples of European soldiers with diarrhea deployed to a field camp in Mali were subjected to whole-genome sequencing (Illumina MiSeq and Oxford Nanopore MinION) and antimicrobial susceptibility testing. With two exemptions, sequence-based analysis suggested an absence of transmission events between soldiers as indicated by a high genetic diversity of isolates and sequence types, confirming previous rep-PCR results. Third-generation cephalosporin resistance was associated with the presence of blaCTX-M-15 genes with (n = 14) and without (n = 5) co-occurring blaTEM-1b genes. Between 0 and 6 virulence and resistance plasmids per isolate were recorded. The detected resistance plasmids could be categorized into five types, which, in turn, share different sequence-identical segments, representing particular antimicrobial resistance gene-associated mobile genetic elements (MGEs). Phenotypic resistance rates within the 19 assessed isolates that showed distinguishable colony morphologies were 94.7% (18/19) against ampicillin-sulbactam and trimethoprim/sulfamethoxazole, 68.4% (13/19) against moxifloxacin, 31.6% (6/19) against ciprofloxacin, 42.1% (8/19) against gentamicin, 31.6% (6/19) against tobramycin, and 21.1% (4/19) against piperacillin-tazobactam and fosfomycin. Virulence-associated genes mediating infectious gastroenteritis were rarely detected. The gene aggR, which is characteristic for enteroaggregative E. coli, was only detected in one single isolate. In summary, we found a variety of different strains and clonal lineages of ESBL-carrying E. coli. Transmission either between soldiers or from common contaminated sources was demonstrated in two cases and played only a minor role in this military field camp, while there were indications that resistance gene bearing MGEs had been exchanged between antimicrobial resistance gene-(ARG-)carrying plasmids.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1169829/fullextended spectrum beta lactamaseESBLEnterobacteralesantibiotic resistancesurveillanceSub-Saharan Africa |
| spellingShingle | Katharina Hoffmann Matthias Riediger Aljoscha Tersteegen Pauline Marquardt Sascha Kahlfuß Sascha Kahlfuß Sascha Kahlfuß Sascha Kahlfuß Achim J. Kaasch Ralf Matthias Hagen Hagen Frickmann Hagen Frickmann Andreas E. Zautner Andreas E. Zautner Molecular epidemiology of enterically colonizing Escherichia coli with resistance against third-generation cephalosporins isolated from stool samples of European soldiers with concomitant diarrhea on deployment in Western African Mali Frontiers in Microbiology extended spectrum beta lactamase ESBL Enterobacterales antibiotic resistance surveillance Sub-Saharan Africa |
| title | Molecular epidemiology of enterically colonizing Escherichia coli with resistance against third-generation cephalosporins isolated from stool samples of European soldiers with concomitant diarrhea on deployment in Western African Mali |
| title_full | Molecular epidemiology of enterically colonizing Escherichia coli with resistance against third-generation cephalosporins isolated from stool samples of European soldiers with concomitant diarrhea on deployment in Western African Mali |
| title_fullStr | Molecular epidemiology of enterically colonizing Escherichia coli with resistance against third-generation cephalosporins isolated from stool samples of European soldiers with concomitant diarrhea on deployment in Western African Mali |
| title_full_unstemmed | Molecular epidemiology of enterically colonizing Escherichia coli with resistance against third-generation cephalosporins isolated from stool samples of European soldiers with concomitant diarrhea on deployment in Western African Mali |
| title_short | Molecular epidemiology of enterically colonizing Escherichia coli with resistance against third-generation cephalosporins isolated from stool samples of European soldiers with concomitant diarrhea on deployment in Western African Mali |
| title_sort | molecular epidemiology of enterically colonizing escherichia coli with resistance against third generation cephalosporins isolated from stool samples of european soldiers with concomitant diarrhea on deployment in western african mali |
| topic | extended spectrum beta lactamase ESBL Enterobacterales antibiotic resistance surveillance Sub-Saharan Africa |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1169829/full |
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