Opportunities in optical and electrical single-cell technologies to study microbial ecosystems
New techniques are revolutionizing single-cell research, allowing us to study microbes at unprecedented scales and in unparalleled depth. This review highlights the state-of-the-art technologies in single-cell analysis in microbial ecology applications, with particular attention to both optical tool...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-08-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1233705/full |
| _version_ | 1797736986374569984 |
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| author | Fabian Mermans Fabian Mermans Valérie Mattelin Ruben Van den Eeckhoudt Cristina García-Timermans Josefien Van Landuyt Yuting Guo Irene Taurino Irene Taurino Filip Tavernier Michael Kraft Michael Kraft Hira Khan Nico Boon |
| author_facet | Fabian Mermans Fabian Mermans Valérie Mattelin Ruben Van den Eeckhoudt Cristina García-Timermans Josefien Van Landuyt Yuting Guo Irene Taurino Irene Taurino Filip Tavernier Michael Kraft Michael Kraft Hira Khan Nico Boon |
| author_sort | Fabian Mermans |
| collection | DOAJ |
| description | New techniques are revolutionizing single-cell research, allowing us to study microbes at unprecedented scales and in unparalleled depth. This review highlights the state-of-the-art technologies in single-cell analysis in microbial ecology applications, with particular attention to both optical tools, i.e., specialized use of flow cytometry and Raman spectroscopy and emerging electrical techniques. The objectives of this review include showcasing the diversity of single-cell optical approaches for studying microbiological phenomena, highlighting successful applications in understanding microbial systems, discussing emerging techniques, and encouraging the combination of established and novel approaches to address research questions. The review aims to answer key questions such as how single-cell approaches have advanced our understanding of individual and interacting cells, how they have been used to study uncultured microbes, which new analysis tools will become widespread, and how they contribute to our knowledge of ecological interactions. |
| first_indexed | 2024-03-12T13:21:51Z |
| format | Article |
| id | doaj.art-eb8c1232c74a4b61bec2976a3fe41338 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-12T13:21:51Z |
| publishDate | 2023-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-eb8c1232c74a4b61bec2976a3fe413382023-08-25T20:10:36ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-08-011410.3389/fmicb.2023.12337051233705Opportunities in optical and electrical single-cell technologies to study microbial ecosystemsFabian Mermans0Fabian Mermans1Valérie Mattelin2Ruben Van den Eeckhoudt3Cristina García-Timermans4Josefien Van Landuyt5Yuting Guo6Irene Taurino7Irene Taurino8Filip Tavernier9Michael Kraft10Michael Kraft11Hira Khan12Nico Boon13Center for Microbial Ecology and Technology (CMET), Department of Biotechnology, Ghent University, Ghent, BelgiumDepartment of Oral Health Sciences, KU Leuven, Leuven, BelgiumCenter for Microbial Ecology and Technology (CMET), Department of Biotechnology, Ghent University, Ghent, BelgiumMicro- and Nanosystems (MNS), Department of Electrical Engineering (ESAT), KU Leuven, Leuven, BelgiumCenter for Microbial Ecology and Technology (CMET), Department of Biotechnology, Ghent University, Ghent, BelgiumCenter for Microbial Ecology and Technology (CMET), Department of Biotechnology, Ghent University, Ghent, BelgiumCenter for Microbial Ecology and Technology (CMET), Department of Biotechnology, Ghent University, Ghent, BelgiumMicro- and Nanosystems (MNS), Department of Electrical Engineering (ESAT), KU Leuven, Leuven, BelgiumSemiconductor Physics, Department of Physics and Astronomy, KU Leuven, Leuven, BelgiumMICAS, Department of Electrical Engineering (ESAT), KU Leuven, Leuven, BelgiumMicro- and Nanosystems (MNS), Department of Electrical Engineering (ESAT), KU Leuven, Leuven, BelgiumLeuven Institute of Micro- and Nanoscale Integration (LIMNI), KU Leuven, Leuven, BelgiumCenter for Microbial Ecology and Technology (CMET), Department of Biotechnology, Ghent University, Ghent, BelgiumCenter for Microbial Ecology and Technology (CMET), Department of Biotechnology, Ghent University, Ghent, BelgiumNew techniques are revolutionizing single-cell research, allowing us to study microbes at unprecedented scales and in unparalleled depth. This review highlights the state-of-the-art technologies in single-cell analysis in microbial ecology applications, with particular attention to both optical tools, i.e., specialized use of flow cytometry and Raman spectroscopy and emerging electrical techniques. The objectives of this review include showcasing the diversity of single-cell optical approaches for studying microbiological phenomena, highlighting successful applications in understanding microbial systems, discussing emerging techniques, and encouraging the combination of established and novel approaches to address research questions. The review aims to answer key questions such as how single-cell approaches have advanced our understanding of individual and interacting cells, how they have been used to study uncultured microbes, which new analysis tools will become widespread, and how they contribute to our knowledge of ecological interactions.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1233705/fullsingle-cellmicrobial ecologyoptical techniqueselectrical techniquesflow cytometryRaman spectroscopy |
| spellingShingle | Fabian Mermans Fabian Mermans Valérie Mattelin Ruben Van den Eeckhoudt Cristina García-Timermans Josefien Van Landuyt Yuting Guo Irene Taurino Irene Taurino Filip Tavernier Michael Kraft Michael Kraft Hira Khan Nico Boon Opportunities in optical and electrical single-cell technologies to study microbial ecosystems Frontiers in Microbiology single-cell microbial ecology optical techniques electrical techniques flow cytometry Raman spectroscopy |
| title | Opportunities in optical and electrical single-cell technologies to study microbial ecosystems |
| title_full | Opportunities in optical and electrical single-cell technologies to study microbial ecosystems |
| title_fullStr | Opportunities in optical and electrical single-cell technologies to study microbial ecosystems |
| title_full_unstemmed | Opportunities in optical and electrical single-cell technologies to study microbial ecosystems |
| title_short | Opportunities in optical and electrical single-cell technologies to study microbial ecosystems |
| title_sort | opportunities in optical and electrical single cell technologies to study microbial ecosystems |
| topic | single-cell microbial ecology optical techniques electrical techniques flow cytometry Raman spectroscopy |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1233705/full |
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