Protocol for diagnosing Erwinia amylovora infection using a fluorescent probe
Summary: Current fire blight diagnosis techniques are DNA based and require specialized equipment and expertise, or they are less sensitive. Here, we present a protocol for diagnosing fire blight using the fluorescent probe, B-1. We describe steps for Erwinia amylovora culture, implementing a fire b...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-09-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166723003799 |
| _version_ | 1797785140178452480 |
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| author | Ji Hye Jin Dokyoung Kim |
| author_facet | Ji Hye Jin Dokyoung Kim |
| author_sort | Ji Hye Jin |
| collection | DOAJ |
| description | Summary: Current fire blight diagnosis techniques are DNA based and require specialized equipment and expertise, or they are less sensitive. Here, we present a protocol for diagnosing fire blight using the fluorescent probe, B-1. We describe steps for Erwinia amylovora culture, implementing a fire blight-infected model, and E. amylovora visualization. This protocol allows for detection of fire blight bacteria of up to 102 CFU/mL on plants or objects in just 10 s with a simple application including spraying and swabbing.For complete details on the use and execution of this protocol, please refer to Jung et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-03-13T00:49:51Z |
| format | Article |
| id | doaj.art-eb8e4ba5d135439983f7e1d863a8ede8 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-03-13T00:49:51Z |
| publishDate | 2023-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-eb8e4ba5d135439983f7e1d863a8ede82023-07-08T04:19:17ZengElsevierSTAR Protocols2666-16672023-09-0143102412Protocol for diagnosing Erwinia amylovora infection using a fluorescent probeJi Hye Jin0Dokyoung Kim1Department of Biomedical Science, Graduate School, Kyung Hee University, Seoul 02447, Republic of KoreaDepartment of Biomedical Science, Graduate School, Kyung Hee University, Seoul 02447, Republic of Korea; Department of Anatomy and Neurobiology, College of Medicine, Kyung Hee University, Seoul 02447, Republic of Korea; Medical Research Center for Bioreaction to Reactive Oxygen Species and Biomedical Science Institute, Core Research Institute (CRI), Kyung Hee University, Seoul 02447, Republic of Korea; Center for Converging Humanities, Kyung Hee University, Seoul 02447, Republic of Korea; KHU-KIST Department of Converging Science and Technology, Kyung Hee University, Seoul 02447, Republic of Korea; UC San Diego Materials Research Science and Engineering Center, 9500 Gilman Drive, La Jolla, CA 92093, USA; Department of Precision Medicine, College of Medicine, Kyung Hee University, Seoul 02447, Republic of Korea; Corresponding authorSummary: Current fire blight diagnosis techniques are DNA based and require specialized equipment and expertise, or they are less sensitive. Here, we present a protocol for diagnosing fire blight using the fluorescent probe, B-1. We describe steps for Erwinia amylovora culture, implementing a fire blight-infected model, and E. amylovora visualization. This protocol allows for detection of fire blight bacteria of up to 102 CFU/mL on plants or objects in just 10 s with a simple application including spraying and swabbing.For complete details on the use and execution of this protocol, please refer to Jung et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166723003799Material SciencesModel OrganismsPlant Sciences |
| spellingShingle | Ji Hye Jin Dokyoung Kim Protocol for diagnosing Erwinia amylovora infection using a fluorescent probe STAR Protocols Material Sciences Model Organisms Plant Sciences |
| title | Protocol for diagnosing Erwinia amylovora infection using a fluorescent probe |
| title_full | Protocol for diagnosing Erwinia amylovora infection using a fluorescent probe |
| title_fullStr | Protocol for diagnosing Erwinia amylovora infection using a fluorescent probe |
| title_full_unstemmed | Protocol for diagnosing Erwinia amylovora infection using a fluorescent probe |
| title_short | Protocol for diagnosing Erwinia amylovora infection using a fluorescent probe |
| title_sort | protocol for diagnosing erwinia amylovora infection using a fluorescent probe |
| topic | Material Sciences Model Organisms Plant Sciences |
| url | http://www.sciencedirect.com/science/article/pii/S2666166723003799 |
| work_keys_str_mv | AT jihyejin protocolfordiagnosingerwiniaamylovorainfectionusingafluorescentprobe AT dokyoungkim protocolfordiagnosingerwiniaamylovorainfectionusingafluorescentprobe |