ON-SITE WHOLE-GENOME SEQUENCING OF SARS-COV-2 REVEALS C3037T MUTATION IN PATIENT WITH ATRIOVENTRICULAR NODAL REENTRY TACHYCARDIA (AVNRT)
Intro: The spike protein of the SARS-CoV-2 virus targets the human cell receptor of angiotensin-converting enzyme (ACE2), including the myocardium and heart's conduction system. Patients diagnosed with COVID-19 have also been found to exhibit cardiac arrhythmia. Here, a whole-genome sequencing...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-05-01
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| Series: | International Journal of Infectious Diseases |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1201971223003739 |
| _version_ | 1797824609478770688 |
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| author | U.A. Zainulabid M.R. Mohd Said H.F. Ahmad |
| author_facet | U.A. Zainulabid M.R. Mohd Said H.F. Ahmad |
| author_sort | U.A. Zainulabid |
| collection | DOAJ |
| description | Intro: The spike protein of the SARS-CoV-2 virus targets the human cell receptor of angiotensin-converting enzyme (ACE2), including the myocardium and heart's conduction system. Patients diagnosed with COVID-19 have also been found to exhibit cardiac arrhythmia. Here, a whole-genome sequencing analysis using long-read sequencing was proposed to evaluate the virus genome in a patient who presented with AVNRT as a main presentation of COVID-19. Methods: The sample was recovered from nasopharyngeal and oropharyngeal swab specimens of a 46-year-old female with no comorbidities who presented with palpitation, and ECG showed typical AVNRT features. The RT-qPCR of SARS- CoV-2 was confirmed positive with a CT-value of 15.82. The total RNAs were extracted and proceeded for RT-qPCR and proceeded with Oxford Nanopore Flongle sequencing. The genomics data of the virus was deposited in GISAID (EPI_ISL_3241561) and further analysed using online bioinformatics tools such as Nextclade CLI 2.3.0. Ethical approval (IREC 2021–080) for the study was obtained from IIUM Research Ethics Committee. Findings: Here, we reported a total of 29,775 bp near-complete whole-genome belonging to clade 21J (Delta) of AY.79 lineage (also known as B.1.617.2.79), which formed a dominant variant in Malaysia during the time of sampling. Discussion: While a previous study showed an association between Delta variant infection with fulminant myocarditis, the present study reported the benign AVNRT as the main presentation of SARS-CoV-2 infection. Furthermore, we observed the presence of the C3037T mutation previously described in the endomyocardial biopsy of a patient with persistent arrhythmia. Conclusion: Even though SARS-CoV-2 targets the respiratory tract, the present study supports the evidence that the ACE2 receptors are present in the heart. In addition, COVID19 is causing more and more damage to heart tissue, and viral transcription has been confirmed on cardiomyocytes. Further functional studies are needed to explore the associated mutations and their relation to cardiac manifestation. |
| first_indexed | 2024-03-13T10:41:32Z |
| format | Article |
| id | doaj.art-eb8feeb8c6394bdba82f8592816b5ce8 |
| institution | Directory Open Access Journal |
| issn | 1201-9712 |
| language | English |
| last_indexed | 2024-03-13T10:41:32Z |
| publishDate | 2023-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | International Journal of Infectious Diseases |
| spelling | doaj.art-eb8feeb8c6394bdba82f8592816b5ce82023-05-18T04:38:46ZengElsevierInternational Journal of Infectious Diseases1201-97122023-05-01130S98ON-SITE WHOLE-GENOME SEQUENCING OF SARS-COV-2 REVEALS C3037T MUTATION IN PATIENT WITH ATRIOVENTRICULAR NODAL REENTRY TACHYCARDIA (AVNRT)U.A. Zainulabid0M.R. Mohd Said1H.F. Ahmad2International Islamic University Malaysia, Internal Medicine, Kulliyyah of Medicine, Kuantan, MalaysiaInternational Islamic University Malaysia, Internal Medicine, Kuantan, MalaysiaUniversiti Malaysia Pahang, Faculty of Industrial Sciences and Technology, Gambang, MalaysiaIntro: The spike protein of the SARS-CoV-2 virus targets the human cell receptor of angiotensin-converting enzyme (ACE2), including the myocardium and heart's conduction system. Patients diagnosed with COVID-19 have also been found to exhibit cardiac arrhythmia. Here, a whole-genome sequencing analysis using long-read sequencing was proposed to evaluate the virus genome in a patient who presented with AVNRT as a main presentation of COVID-19. Methods: The sample was recovered from nasopharyngeal and oropharyngeal swab specimens of a 46-year-old female with no comorbidities who presented with palpitation, and ECG showed typical AVNRT features. The RT-qPCR of SARS- CoV-2 was confirmed positive with a CT-value of 15.82. The total RNAs were extracted and proceeded for RT-qPCR and proceeded with Oxford Nanopore Flongle sequencing. The genomics data of the virus was deposited in GISAID (EPI_ISL_3241561) and further analysed using online bioinformatics tools such as Nextclade CLI 2.3.0. Ethical approval (IREC 2021–080) for the study was obtained from IIUM Research Ethics Committee. Findings: Here, we reported a total of 29,775 bp near-complete whole-genome belonging to clade 21J (Delta) of AY.79 lineage (also known as B.1.617.2.79), which formed a dominant variant in Malaysia during the time of sampling. Discussion: While a previous study showed an association between Delta variant infection with fulminant myocarditis, the present study reported the benign AVNRT as the main presentation of SARS-CoV-2 infection. Furthermore, we observed the presence of the C3037T mutation previously described in the endomyocardial biopsy of a patient with persistent arrhythmia. Conclusion: Even though SARS-CoV-2 targets the respiratory tract, the present study supports the evidence that the ACE2 receptors are present in the heart. In addition, COVID19 is causing more and more damage to heart tissue, and viral transcription has been confirmed on cardiomyocytes. Further functional studies are needed to explore the associated mutations and their relation to cardiac manifestation.http://www.sciencedirect.com/science/article/pii/S1201971223003739 |
| spellingShingle | U.A. Zainulabid M.R. Mohd Said H.F. Ahmad ON-SITE WHOLE-GENOME SEQUENCING OF SARS-COV-2 REVEALS C3037T MUTATION IN PATIENT WITH ATRIOVENTRICULAR NODAL REENTRY TACHYCARDIA (AVNRT) International Journal of Infectious Diseases |
| title | ON-SITE WHOLE-GENOME SEQUENCING OF SARS-COV-2 REVEALS C3037T MUTATION IN PATIENT WITH ATRIOVENTRICULAR NODAL REENTRY TACHYCARDIA (AVNRT) |
| title_full | ON-SITE WHOLE-GENOME SEQUENCING OF SARS-COV-2 REVEALS C3037T MUTATION IN PATIENT WITH ATRIOVENTRICULAR NODAL REENTRY TACHYCARDIA (AVNRT) |
| title_fullStr | ON-SITE WHOLE-GENOME SEQUENCING OF SARS-COV-2 REVEALS C3037T MUTATION IN PATIENT WITH ATRIOVENTRICULAR NODAL REENTRY TACHYCARDIA (AVNRT) |
| title_full_unstemmed | ON-SITE WHOLE-GENOME SEQUENCING OF SARS-COV-2 REVEALS C3037T MUTATION IN PATIENT WITH ATRIOVENTRICULAR NODAL REENTRY TACHYCARDIA (AVNRT) |
| title_short | ON-SITE WHOLE-GENOME SEQUENCING OF SARS-COV-2 REVEALS C3037T MUTATION IN PATIENT WITH ATRIOVENTRICULAR NODAL REENTRY TACHYCARDIA (AVNRT) |
| title_sort | on site whole genome sequencing of sars cov 2 reveals c3037t mutation in patient with atrioventricular nodal reentry tachycardia avnrt |
| url | http://www.sciencedirect.com/science/article/pii/S1201971223003739 |
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