Blood-Glucose-Lowering Effect of Coptidis Rhizoma Extracts From Different Origins via Gut Microbiota Modulation in db/db Mice
Background:Coptidis rhizoma extracts (CREs) have been used widely for their anti-diabetic and anti-microbial activities, and berberine/jatrorrhizine/coptisine/palmatine are the primary bioactive components. Although guidelines have adopted content analyses of these components as a quality control me...
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| Format: | Article |
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Frontiers Media S.A.
2021-06-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2021.684358/full |
| _version_ | 1818407104909672448 |
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| author | Yuanfeng Lyu Lin Lin Yuning Xie Dan Li Min Xiao Yufeng Zhang Stanley Chun Kai Cheung Pang Chui Shaw Xiao Yang Paul Kay Sheung Chan Alice Pik Shan Kong Zhong Zuo |
| author_facet | Yuanfeng Lyu Lin Lin Yuning Xie Dan Li Min Xiao Yufeng Zhang Stanley Chun Kai Cheung Pang Chui Shaw Xiao Yang Paul Kay Sheung Chan Alice Pik Shan Kong Zhong Zuo |
| author_sort | Yuanfeng Lyu |
| collection | DOAJ |
| description | Background:Coptidis rhizoma extracts (CREs) have been used widely for their anti-diabetic and anti-microbial activities, and berberine/jatrorrhizine/coptisine/palmatine are the primary bioactive components. Although guidelines have adopted content analyses of these components as a quality control method for CREs, it is difficult to differentiate the CREs from different sources using this method because of the lack of indications for their related pharmacological activities.Purpose: To explore the effect of CREs (CREA/CREB/CREC) with different compositions of major components on the gut microbiota and blood glucose levels in db/db mice.Methods: Degradation of berberine/jatrorrhizine/coptisine/palmatine from CREA/CREB/CREC in rat/mouse intestinal contents and their impact on nine common gastrointestinal bacteria were investigated. In addition, the effects of oral administration of CREA/CREB/CREC for 2 weeks on the gut microbiota and blood glucose levels in db/db mice were monitored via insulin/glucose tolerance test (ITT/GTT), insulin concentration, homeostatic model assessment of insulin resistance and fecal 16S rRNA sequencing.Results and Conclusion: The total amount of berberine/jatrorrhizine/coptisine/palmatine was highest in CREA. Clostridium perfringens was strongly inhibited by all three CREs, with CREA demonstrating the most significant inhibitory effects on minimum inhibitory concentration, time-kill kinetics, and ATP production. In db/db mice, CREA resulted in the most significant decrease in ITT/GTT and depicted different changes in the microbiota from CREB/CREC. Thus, CREs with different compositions of berberine/jatrorrhizine/coptisine/palmatine differed in terms of time-kill kinetics and ATP production assays on C. perfringens. CREA revealed the potent bacterial inhibitory effects and glucose-lowering activity. |
| first_indexed | 2024-12-14T09:22:32Z |
| format | Article |
| id | doaj.art-eb92e8ac24484bbabd90bd9087d12ab5 |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-12-14T09:22:32Z |
| publishDate | 2021-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-eb92e8ac24484bbabd90bd9087d12ab52022-12-21T23:08:17ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122021-06-011210.3389/fphar.2021.684358684358Blood-Glucose-Lowering Effect of Coptidis Rhizoma Extracts From Different Origins via Gut Microbiota Modulation in db/db MiceYuanfeng Lyu0Lin Lin1Yuning Xie2Dan Li3Min Xiao4Yufeng Zhang5Stanley Chun Kai Cheung6Pang Chui Shaw7Xiao Yang8Paul Kay Sheung Chan9Alice Pik Shan Kong10Zhong Zuo11School of Pharmacy, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, ChinaDepartment of Microbiology, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, ChinaSchool of Pharmacy, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, ChinaSchool of Pharmacy, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, ChinaSchool of Pharmacy, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, ChinaSchool of Pharmacy, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, ChinaSchool of Life Sciences and Li Dak Sum Yip Yio Chin R&D Centre for Chinese Medicine, The Chinese University of Hong Kong, Shatin, ChinaSchool of Life Sciences and Li Dak Sum Yip Yio Chin R&D Centre for Chinese Medicine, The Chinese University of Hong Kong, Shatin, ChinaDepartment of Microbiology, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, ChinaDepartment of Microbiology, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, ChinaDivision of Endocrinology, Department of Medicine and Therapeutics, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, ChinaSchool of Pharmacy, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, ChinaBackground:Coptidis rhizoma extracts (CREs) have been used widely for their anti-diabetic and anti-microbial activities, and berberine/jatrorrhizine/coptisine/palmatine are the primary bioactive components. Although guidelines have adopted content analyses of these components as a quality control method for CREs, it is difficult to differentiate the CREs from different sources using this method because of the lack of indications for their related pharmacological activities.Purpose: To explore the effect of CREs (CREA/CREB/CREC) with different compositions of major components on the gut microbiota and blood glucose levels in db/db mice.Methods: Degradation of berberine/jatrorrhizine/coptisine/palmatine from CREA/CREB/CREC in rat/mouse intestinal contents and their impact on nine common gastrointestinal bacteria were investigated. In addition, the effects of oral administration of CREA/CREB/CREC for 2 weeks on the gut microbiota and blood glucose levels in db/db mice were monitored via insulin/glucose tolerance test (ITT/GTT), insulin concentration, homeostatic model assessment of insulin resistance and fecal 16S rRNA sequencing.Results and Conclusion: The total amount of berberine/jatrorrhizine/coptisine/palmatine was highest in CREA. Clostridium perfringens was strongly inhibited by all three CREs, with CREA demonstrating the most significant inhibitory effects on minimum inhibitory concentration, time-kill kinetics, and ATP production. In db/db mice, CREA resulted in the most significant decrease in ITT/GTT and depicted different changes in the microbiota from CREB/CREC. Thus, CREs with different compositions of berberine/jatrorrhizine/coptisine/palmatine differed in terms of time-kill kinetics and ATP production assays on C. perfringens. CREA revealed the potent bacterial inhibitory effects and glucose-lowering activity.https://www.frontiersin.org/articles/10.3389/fphar.2021.684358/fullCoptidis rhizoma extractsgastrointestinal bacteriagut microbiotadb/db micediabetes |
| spellingShingle | Yuanfeng Lyu Lin Lin Yuning Xie Dan Li Min Xiao Yufeng Zhang Stanley Chun Kai Cheung Pang Chui Shaw Xiao Yang Paul Kay Sheung Chan Alice Pik Shan Kong Zhong Zuo Blood-Glucose-Lowering Effect of Coptidis Rhizoma Extracts From Different Origins via Gut Microbiota Modulation in db/db Mice Frontiers in Pharmacology Coptidis rhizoma extracts gastrointestinal bacteria gut microbiota db/db mice diabetes |
| title | Blood-Glucose-Lowering Effect of Coptidis Rhizoma Extracts From Different Origins via Gut Microbiota Modulation in db/db Mice |
| title_full | Blood-Glucose-Lowering Effect of Coptidis Rhizoma Extracts From Different Origins via Gut Microbiota Modulation in db/db Mice |
| title_fullStr | Blood-Glucose-Lowering Effect of Coptidis Rhizoma Extracts From Different Origins via Gut Microbiota Modulation in db/db Mice |
| title_full_unstemmed | Blood-Glucose-Lowering Effect of Coptidis Rhizoma Extracts From Different Origins via Gut Microbiota Modulation in db/db Mice |
| title_short | Blood-Glucose-Lowering Effect of Coptidis Rhizoma Extracts From Different Origins via Gut Microbiota Modulation in db/db Mice |
| title_sort | blood glucose lowering effect of coptidis rhizoma extracts from different origins via gut microbiota modulation in db db mice |
| topic | Coptidis rhizoma extracts gastrointestinal bacteria gut microbiota db/db mice diabetes |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2021.684358/full |
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