Effect of injection of L-NAME on drinking response
The drinking behavior responses to centrally administered NG-nitro-L-arginine methyl ester (L-NAME; 10, 20 or 40 µg/µl), an inhibitor of nitric oxide synthase, were studied in satiated rats, with cannulae stereotaxically implanted into the lateral ventricle (LV) and subfornical organ (SFO). Water in...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Divulgação Científica
1999-11-01
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| Series: | Brazilian Journal of Medical and Biological Research |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001100012 |
| _version_ | 1811293239755407360 |
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| author | W.A. Saad L.A.A. Camargo R. Saad A.F. Pereira S. Simões |
| author_facet | W.A. Saad L.A.A. Camargo R. Saad A.F. Pereira S. Simões |
| author_sort | W.A. Saad |
| collection | DOAJ |
| description | The drinking behavior responses to centrally administered NG-nitro-L-arginine methyl ester (L-NAME; 10, 20 or 40 µg/µl), an inhibitor of nitric oxide synthase, were studied in satiated rats, with cannulae stereotaxically implanted into the lateral ventricle (LV) and subfornical organ (SFO). Water intake increased in all animals after angiotensin II (ANG II) injection into the LV, with values of 14.2 ± 1.4 ml/h. After injection of L-NAME at doses of 10, 20 or 40 µg/µl into the SFO before injection of ANG II (12 ng/µl) into the LV, water intake decreased progressively and reached basal levels after treatment with 0.15 M NaCl and with the highest dose of L-NAME (i.e., 40 µg). The water intake obtained after 40 µg/µl L-NAME was 0.8 ± 0.01 ml/h. Also, the injection of L-NAME, 10, 20 or 40 µg/µl, into the LV progressively reduced the water intake induced by hypertonic saline, with values of 5.3 ± 0.8, 3.2 ± 0.8 and 0.7 ± 0.01 ml/h, respectively. These results indicate that nitric oxide is involved in the regulation of drinking behavior induced by centrally administered ANG II and cellular dehydration and that the nitric oxide of the SFO plays an important role in this regulation. |
| first_indexed | 2024-04-13T04:58:29Z |
| format | Article |
| id | doaj.art-eb9342424faa49318b1c330a9cde34e2 |
| institution | Directory Open Access Journal |
| issn | 0100-879X 1414-431X |
| language | English |
| last_indexed | 2024-04-13T04:58:29Z |
| publishDate | 1999-11-01 |
| publisher | Associação Brasileira de Divulgação Científica |
| record_format | Article |
| series | Brazilian Journal of Medical and Biological Research |
| spelling | doaj.art-eb9342424faa49318b1c330a9cde34e22022-12-22T03:01:25ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research0100-879X1414-431X1999-11-0132111413141610.1590/S0100-879X1999001100012Effect of injection of L-NAME on drinking responseW.A. SaadL.A.A. CamargoR. SaadA.F. PereiraS. SimõesThe drinking behavior responses to centrally administered NG-nitro-L-arginine methyl ester (L-NAME; 10, 20 or 40 µg/µl), an inhibitor of nitric oxide synthase, were studied in satiated rats, with cannulae stereotaxically implanted into the lateral ventricle (LV) and subfornical organ (SFO). Water intake increased in all animals after angiotensin II (ANG II) injection into the LV, with values of 14.2 ± 1.4 ml/h. After injection of L-NAME at doses of 10, 20 or 40 µg/µl into the SFO before injection of ANG II (12 ng/µl) into the LV, water intake decreased progressively and reached basal levels after treatment with 0.15 M NaCl and with the highest dose of L-NAME (i.e., 40 µg). The water intake obtained after 40 µg/µl L-NAME was 0.8 ± 0.01 ml/h. Also, the injection of L-NAME, 10, 20 or 40 µg/µl, into the LV progressively reduced the water intake induced by hypertonic saline, with values of 5.3 ± 0.8, 3.2 ± 0.8 and 0.7 ± 0.01 ml/h, respectively. These results indicate that nitric oxide is involved in the regulation of drinking behavior induced by centrally administered ANG II and cellular dehydration and that the nitric oxide of the SFO plays an important role in this regulation.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001100012nitric oxidewater intakeSFOdehydrationCNS |
| spellingShingle | W.A. Saad L.A.A. Camargo R. Saad A.F. Pereira S. Simões Effect of injection of L-NAME on drinking response Brazilian Journal of Medical and Biological Research nitric oxide water intake SFO dehydration CNS |
| title | Effect of injection of L-NAME on drinking response |
| title_full | Effect of injection of L-NAME on drinking response |
| title_fullStr | Effect of injection of L-NAME on drinking response |
| title_full_unstemmed | Effect of injection of L-NAME on drinking response |
| title_short | Effect of injection of L-NAME on drinking response |
| title_sort | effect of injection of l name on drinking response |
| topic | nitric oxide water intake SFO dehydration CNS |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001100012 |
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