Modulation of Synthetic Tracheal Grafts with Extracellular Matrix Coatings
Synthetic scaffolds for the repair of long-segment tracheal defects are hindered by insufficient biocompatibility and poor graft epithelialization. In this study, we determined if extracellular matrix (ECM) coatings improved the biocompatibility and epithelialization of synthetic tracheal grafts (sy...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-08-01
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| Series: | Bioengineering |
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| Online Access: | https://www.mdpi.com/2306-5354/8/8/116 |
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| author | Lumei Liu Sayali Dharmadhikari Robert A. Pouliot Michael M. Li Peter M. Minneci Zhenghong Tan Kimberly Shontz Jed Johnson Susan D. Reynolds Christopher K. Breuer Daniel J. Weiss Tendy Chiang |
| author_facet | Lumei Liu Sayali Dharmadhikari Robert A. Pouliot Michael M. Li Peter M. Minneci Zhenghong Tan Kimberly Shontz Jed Johnson Susan D. Reynolds Christopher K. Breuer Daniel J. Weiss Tendy Chiang |
| author_sort | Lumei Liu |
| collection | DOAJ |
| description | Synthetic scaffolds for the repair of long-segment tracheal defects are hindered by insufficient biocompatibility and poor graft epithelialization. In this study, we determined if extracellular matrix (ECM) coatings improved the biocompatibility and epithelialization of synthetic tracheal grafts (syn-TG). Porcine and human ECM substrates (pECM and hECM) were created through the decellularization and lyophilization of lung tissue. Four concentrations of pECM and hECM coatings on syn-TG were characterized for their effects on scaffold morphologies and on in vitro cell viability and growth. Uncoated and ECM-coated syn-TG were subsequently evaluated in vivo through the orthotopic implantation of segmental grafts or patches. These studies demonstrated that ECM coatings were not cytotoxic and, enhanced the in vitro cell viability and growth on syn-TG in a dose-dependent manner. Mass spectrometry demonstrated that fibrillin, collagen, laminin, and nephronectin were the predominant ECM components transferred onto scaffolds. The in vivo results exhibited similar robust epithelialization of uncoated and coated syn-TG patches; however, the epithelialization remained poor with either uncoated or coated scaffolds in the segmental replacement models. Overall, these findings demonstrated that ECM coatings improve the seeded cell biocompatibility of synthetic scaffolds in vitro; however, they do not improve graft epithelialization in vivo. |
| first_indexed | 2024-03-10T09:00:54Z |
| format | Article |
| id | doaj.art-eb93d533b79e4011a9d32ee721dd0c94 |
| institution | Directory Open Access Journal |
| issn | 2306-5354 |
| language | English |
| last_indexed | 2024-03-10T09:00:54Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Bioengineering |
| spelling | doaj.art-eb93d533b79e4011a9d32ee721dd0c942023-11-22T06:49:09ZengMDPI AGBioengineering2306-53542021-08-018811610.3390/bioengineering8080116Modulation of Synthetic Tracheal Grafts with Extracellular Matrix CoatingsLumei Liu0Sayali Dharmadhikari1Robert A. Pouliot2Michael M. Li3Peter M. Minneci4Zhenghong Tan5Kimberly Shontz6Jed Johnson7Susan D. Reynolds8Christopher K. Breuer9Daniel J. Weiss10Tendy Chiang11Center of Regenerative Medicine, Abigail Wexner Research Institute, Nationwide Children’s Hospital, Columbus, OH 43215, USACenter of Regenerative Medicine, Abigail Wexner Research Institute, Nationwide Children’s Hospital, Columbus, OH 43215, USADepartment of Medicine, Larner College of Medicine, University of Vermont, Burlington, VT 05405, USADepartment of Pediatric Otolaryngology, Nationwide Children’s Hospital, Columbus, OH 43205, USACenter of Regenerative Medicine, Abigail Wexner Research Institute, Nationwide Children’s Hospital, Columbus, OH 43215, USACollege of Medicine, The Ohio State University, Columbus, OH 43210, USACenter of Regenerative Medicine, Abigail Wexner Research Institute, Nationwide Children’s Hospital, Columbus, OH 43215, USANanofiber Solutions, Inc., Columbus, OH 43017, USACenter for Perinatal Research, Abigail Wexner Research Institute, Nationwide Children’s Hospital, Columbus, OH 43215, USACenter of Regenerative Medicine, Abigail Wexner Research Institute, Nationwide Children’s Hospital, Columbus, OH 43215, USADepartment of Pediatric Otolaryngology, Nationwide Children’s Hospital, Columbus, OH 43205, USACenter of Regenerative Medicine, Abigail Wexner Research Institute, Nationwide Children’s Hospital, Columbus, OH 43215, USASynthetic scaffolds for the repair of long-segment tracheal defects are hindered by insufficient biocompatibility and poor graft epithelialization. In this study, we determined if extracellular matrix (ECM) coatings improved the biocompatibility and epithelialization of synthetic tracheal grafts (syn-TG). Porcine and human ECM substrates (pECM and hECM) were created through the decellularization and lyophilization of lung tissue. Four concentrations of pECM and hECM coatings on syn-TG were characterized for their effects on scaffold morphologies and on in vitro cell viability and growth. Uncoated and ECM-coated syn-TG were subsequently evaluated in vivo through the orthotopic implantation of segmental grafts or patches. These studies demonstrated that ECM coatings were not cytotoxic and, enhanced the in vitro cell viability and growth on syn-TG in a dose-dependent manner. Mass spectrometry demonstrated that fibrillin, collagen, laminin, and nephronectin were the predominant ECM components transferred onto scaffolds. The in vivo results exhibited similar robust epithelialization of uncoated and coated syn-TG patches; however, the epithelialization remained poor with either uncoated or coated scaffolds in the segmental replacement models. Overall, these findings demonstrated that ECM coatings improve the seeded cell biocompatibility of synthetic scaffolds in vitro; however, they do not improve graft epithelialization in vivo.https://www.mdpi.com/2306-5354/8/8/116cell viabilitybiocompatibilityextracellular matrix coatingsynthetic tracheal grafttracheal regenerationelectrospinning |
| spellingShingle | Lumei Liu Sayali Dharmadhikari Robert A. Pouliot Michael M. Li Peter M. Minneci Zhenghong Tan Kimberly Shontz Jed Johnson Susan D. Reynolds Christopher K. Breuer Daniel J. Weiss Tendy Chiang Modulation of Synthetic Tracheal Grafts with Extracellular Matrix Coatings Bioengineering cell viability biocompatibility extracellular matrix coating synthetic tracheal graft tracheal regeneration electrospinning |
| title | Modulation of Synthetic Tracheal Grafts with Extracellular Matrix Coatings |
| title_full | Modulation of Synthetic Tracheal Grafts with Extracellular Matrix Coatings |
| title_fullStr | Modulation of Synthetic Tracheal Grafts with Extracellular Matrix Coatings |
| title_full_unstemmed | Modulation of Synthetic Tracheal Grafts with Extracellular Matrix Coatings |
| title_short | Modulation of Synthetic Tracheal Grafts with Extracellular Matrix Coatings |
| title_sort | modulation of synthetic tracheal grafts with extracellular matrix coatings |
| topic | cell viability biocompatibility extracellular matrix coating synthetic tracheal graft tracheal regeneration electrospinning |
| url | https://www.mdpi.com/2306-5354/8/8/116 |
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