The Rx Gene Confers Resistance to a Range of Potexviruses in Transgenic Nicotiana Plants
Rx-mediated resistance was analyzed in Rx-expressing transgenic Nicotiana plants. The infection outcome of nine Potato virus X isolates mutated at amino acid positions 121 and 127 of the coat protein (CP) confirmed the key role of these amino acids but provided a more complex picture than previously...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2008-09-01
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| Series: | Molecular Plant-Microbe Interactions |
| Subjects: | |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI-21-9-1154 |
| _version_ | 1811287345625825280 |
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| author | Isabelle Baurès Thierry Candresse Aymeric Leveau Abdelhafid Bendahmane Bénédicte Sturbois |
| author_facet | Isabelle Baurès Thierry Candresse Aymeric Leveau Abdelhafid Bendahmane Bénédicte Sturbois |
| author_sort | Isabelle Baurès |
| collection | DOAJ |
| description | Rx-mediated resistance was analyzed in Rx-expressing transgenic Nicotiana plants. The infection outcome of nine Potato virus X isolates mutated at amino acid positions 121 and 127 of the coat protein (CP) confirmed the key role of these amino acids but provided a more complex picture than previously reported. In particular, in Rx-expressing Nicotiana spp., eliciting activity modulated by amino acid 121 was conditioned by the nature of amino acid 127. These results suggest that the specificity of recognition might be modulated by host factors that are somehow subtly modified between Rx-expressing potato and Rx-expressing transgenic Nicotiana plants. Moreover, the CP of three Potexviruses, Narcissus mosaic virus (NMV), White clover mosaic virus (WClMV), and Cymbidium mosaic virus (CymMV), are all recognized by the Rx-based machinery and able to trigger an Rx-dependant hypersensitive response. A smaller elicitor of 90 amino acids was identified in the CP of NMV and WClMV, which contains the previously identified key positions 121 and 127. This elicitor is only weakly conserved (approximately 40% identity) among the CP of the various recognized viruses, suggesting that the Rx molecular machinery targets a conserved structural element of the Potexvirus CP rather than a conserved amino acid motif. |
| first_indexed | 2024-04-13T03:16:55Z |
| format | Article |
| id | doaj.art-eb93ec3fde764a4c96e359d04673d3e8 |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-04-13T03:16:55Z |
| publishDate | 2008-09-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-eb93ec3fde764a4c96e359d04673d3e82022-12-22T03:04:54ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062008-09-012191154116410.1094/MPMI-21-9-1154The Rx Gene Confers Resistance to a Range of Potexviruses in Transgenic Nicotiana PlantsIsabelle BaurèsThierry CandresseAymeric LeveauAbdelhafid BendahmaneBénédicte SturboisRx-mediated resistance was analyzed in Rx-expressing transgenic Nicotiana plants. The infection outcome of nine Potato virus X isolates mutated at amino acid positions 121 and 127 of the coat protein (CP) confirmed the key role of these amino acids but provided a more complex picture than previously reported. In particular, in Rx-expressing Nicotiana spp., eliciting activity modulated by amino acid 121 was conditioned by the nature of amino acid 127. These results suggest that the specificity of recognition might be modulated by host factors that are somehow subtly modified between Rx-expressing potato and Rx-expressing transgenic Nicotiana plants. Moreover, the CP of three Potexviruses, Narcissus mosaic virus (NMV), White clover mosaic virus (WClMV), and Cymbidium mosaic virus (CymMV), are all recognized by the Rx-based machinery and able to trigger an Rx-dependant hypersensitive response. A smaller elicitor of 90 amino acids was identified in the CP of NMV and WClMV, which contains the previously identified key positions 121 and 127. This elicitor is only weakly conserved (approximately 40% identity) among the CP of the various recognized viruses, suggesting that the Rx molecular machinery targets a conserved structural element of the Potexvirus CP rather than a conserved amino acid motif.https://apsjournals.apsnet.org/doi/10.1094/MPMI-21-9-1154extreme resistancehypersensitive response |
| spellingShingle | Isabelle Baurès Thierry Candresse Aymeric Leveau Abdelhafid Bendahmane Bénédicte Sturbois The Rx Gene Confers Resistance to a Range of Potexviruses in Transgenic Nicotiana Plants Molecular Plant-Microbe Interactions extreme resistance hypersensitive response |
| title | The Rx Gene Confers Resistance to a Range of Potexviruses in Transgenic Nicotiana Plants |
| title_full | The Rx Gene Confers Resistance to a Range of Potexviruses in Transgenic Nicotiana Plants |
| title_fullStr | The Rx Gene Confers Resistance to a Range of Potexviruses in Transgenic Nicotiana Plants |
| title_full_unstemmed | The Rx Gene Confers Resistance to a Range of Potexviruses in Transgenic Nicotiana Plants |
| title_short | The Rx Gene Confers Resistance to a Range of Potexviruses in Transgenic Nicotiana Plants |
| title_sort | rx gene confers resistance to a range of potexviruses in transgenic nicotiana plants |
| topic | extreme resistance hypersensitive response |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI-21-9-1154 |
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