Characterization of PVL-Positive MRSA Isolates in Northern Bavaria, Germany over an Eight-Year Period
Purpose: Community-acquired methicillin-resistant <i>Staphylococcus aureus</i> strains (CA-MRSA) are spread worldwide and often cause recurring and persistent infections in humans. CA-MRSA strains frequently carry Panton–Valentine leukocidin (PVL) as a distinctive virulence factor. This...
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MDPI AG
2022-12-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/1/54 |
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| author | Tobias Szumlanski Bernd Neumann Ralph Bertram Alexandra Simbeck Renate Ziegler Stefan Monecke Ralf Ehricht Wulf Schneider-Brachert Joerg Steinmann |
| author_facet | Tobias Szumlanski Bernd Neumann Ralph Bertram Alexandra Simbeck Renate Ziegler Stefan Monecke Ralf Ehricht Wulf Schneider-Brachert Joerg Steinmann |
| author_sort | Tobias Szumlanski |
| collection | DOAJ |
| description | Purpose: Community-acquired methicillin-resistant <i>Staphylococcus aureus</i> strains (CA-MRSA) are spread worldwide and often cause recurring and persistent infections in humans. CA-MRSA strains frequently carry Panton–Valentine leukocidin (PVL) as a distinctive virulence factor. This study investigates the molecular epidemiology, antibiotic resistance and clinical characteristics of PVL-positive MRSA strains in Northern Bavaria, Germany, isolated over an eight-year period. Methods: Strains were identified by MALDI-TOF MS and antibiotic susceptibility was tested by automated microdilution (VITEK 2) or disk diffusion. PVL-encoding genes and <i>mecA</i> were detected by PCR. MRSA clonal complexes (CC) and lineages were assigned by genotyping via DNA microarray and <i>spa</i>-typing. Results: In total, 131 PVL-positive MRSA were collected from five hospital sites between 2009 and 2016. Predominant lineages were CC8-MRSA-[IV+ACME], USA300 (27/131; 20.6%); CC30-MRSA-IV, Southwest Pacific Clone (26/131; 19.8%) and CC80-MRSA-IV (25/131; 19.1%). Other CCs were detected less frequently. Resistance against erythromycin and clindamycin was prevalent, whereas all strains were sensitive towards vancomycin and linezolid. In total, 100 cases (76.3%) were causally linked to an infection. The majority (102/131; 77.9%) of isolates were detected in skin swabs or swabs from surgical sites. Conclusions: During the sample period we found an increase in the PVL-positive MRSA lineages CC30 and CC1. Compared to less-abundant lineages CC1 or CC22, the predominant lineages CC8, CC30 and CC80 harbored a broader resistance spectrum. Furthermore, these lineages are probably associated with a travel and migration background. In the spatio-temporal setting we investigated, these were arguably drivers of diversification and change in the landscape of PVL-positive MRSA. |
| first_indexed | 2024-03-09T11:39:41Z |
| format | Article |
| id | doaj.art-eb9c976ba0ab4cefadf69e91e404364a |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T11:39:41Z |
| publishDate | 2022-12-01 |
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| series | Microorganisms |
| spelling | doaj.art-eb9c976ba0ab4cefadf69e91e404364a2023-11-30T23:35:44ZengMDPI AGMicroorganisms2076-26072022-12-011115410.3390/microorganisms11010054Characterization of PVL-Positive MRSA Isolates in Northern Bavaria, Germany over an Eight-Year PeriodTobias Szumlanski0Bernd Neumann1Ralph Bertram2Alexandra Simbeck3Renate Ziegler4Stefan Monecke5Ralf Ehricht6Wulf Schneider-Brachert7Joerg Steinmann8Institute for Hospital Hygiene, Medical Microbiology and Clinical Infectiology, Paracelsus Medical University, Nuremberg General Hospital, 90419 Nuremberg, GermanyInstitute for Hospital Hygiene, Medical Microbiology and Clinical Infectiology, Paracelsus Medical University, Nuremberg General Hospital, 90419 Nuremberg, GermanyInstitute for Hospital Hygiene, Medical Microbiology and Clinical Infectiology, Paracelsus Medical University, Nuremberg General Hospital, 90419 Nuremberg, GermanyDepartment of Surgery, Asklepios Hospital Barmbek, 22307 Hamburg, GermanyInstitute for Hospital Hygiene, Medical Microbiology and Clinical Infectiology, Paracelsus Medical University, Nuremberg General Hospital, 90419 Nuremberg, GermanyLeibniz Institute of Photonic Technology (IPHT), 07745 Jena, GermanyLeibniz Institute of Photonic Technology (IPHT), 07745 Jena, GermanyDepartment of Infection Prevention and Infectious Diseases, University Hospital Regensburg, 93053 Regensburg, GermanyInstitute for Hospital Hygiene, Medical Microbiology and Clinical Infectiology, Paracelsus Medical University, Nuremberg General Hospital, 90419 Nuremberg, GermanyPurpose: Community-acquired methicillin-resistant <i>Staphylococcus aureus</i> strains (CA-MRSA) are spread worldwide and often cause recurring and persistent infections in humans. CA-MRSA strains frequently carry Panton–Valentine leukocidin (PVL) as a distinctive virulence factor. This study investigates the molecular epidemiology, antibiotic resistance and clinical characteristics of PVL-positive MRSA strains in Northern Bavaria, Germany, isolated over an eight-year period. Methods: Strains were identified by MALDI-TOF MS and antibiotic susceptibility was tested by automated microdilution (VITEK 2) or disk diffusion. PVL-encoding genes and <i>mecA</i> were detected by PCR. MRSA clonal complexes (CC) and lineages were assigned by genotyping via DNA microarray and <i>spa</i>-typing. Results: In total, 131 PVL-positive MRSA were collected from five hospital sites between 2009 and 2016. Predominant lineages were CC8-MRSA-[IV+ACME], USA300 (27/131; 20.6%); CC30-MRSA-IV, Southwest Pacific Clone (26/131; 19.8%) and CC80-MRSA-IV (25/131; 19.1%). Other CCs were detected less frequently. Resistance against erythromycin and clindamycin was prevalent, whereas all strains were sensitive towards vancomycin and linezolid. In total, 100 cases (76.3%) were causally linked to an infection. The majority (102/131; 77.9%) of isolates were detected in skin swabs or swabs from surgical sites. Conclusions: During the sample period we found an increase in the PVL-positive MRSA lineages CC30 and CC1. Compared to less-abundant lineages CC1 or CC22, the predominant lineages CC8, CC30 and CC80 harbored a broader resistance spectrum. Furthermore, these lineages are probably associated with a travel and migration background. In the spatio-temporal setting we investigated, these were arguably drivers of diversification and change in the landscape of PVL-positive MRSA.https://www.mdpi.com/2076-2607/11/1/54CA-MRSAPVLantimicrobial resistancetravelmigration |
| spellingShingle | Tobias Szumlanski Bernd Neumann Ralph Bertram Alexandra Simbeck Renate Ziegler Stefan Monecke Ralf Ehricht Wulf Schneider-Brachert Joerg Steinmann Characterization of PVL-Positive MRSA Isolates in Northern Bavaria, Germany over an Eight-Year Period Microorganisms CA-MRSA PVL antimicrobial resistance travel migration |
| title | Characterization of PVL-Positive MRSA Isolates in Northern Bavaria, Germany over an Eight-Year Period |
| title_full | Characterization of PVL-Positive MRSA Isolates in Northern Bavaria, Germany over an Eight-Year Period |
| title_fullStr | Characterization of PVL-Positive MRSA Isolates in Northern Bavaria, Germany over an Eight-Year Period |
| title_full_unstemmed | Characterization of PVL-Positive MRSA Isolates in Northern Bavaria, Germany over an Eight-Year Period |
| title_short | Characterization of PVL-Positive MRSA Isolates in Northern Bavaria, Germany over an Eight-Year Period |
| title_sort | characterization of pvl positive mrsa isolates in northern bavaria germany over an eight year period |
| topic | CA-MRSA PVL antimicrobial resistance travel migration |
| url | https://www.mdpi.com/2076-2607/11/1/54 |
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