<i>Sloanea chocoana</i> and <i>S. pittieriana</i> (Elaeocarpaceae): Chemical and Biological Studies of Ethanolic Extracts and Skincare Properties
The Colombian Chocó is known for its rich biodiversity and to harbor plant species that are under-explored, including the genus <i>Sloanea</i>. This study aimed to analyze the chemical composition of derivatized ethanolic extracts from <i>S. chocoana</i> and <i>S. pitti...
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MDPI AG
2023-11-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/23/3953 |
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| author | Patricia Quintero-Rincón Nayive Pino-Benítez Elkin Galeano Cris Rojo-Uribe Ana C. Mesa-Arango Oscar A. Flórez-Acosta |
| author_facet | Patricia Quintero-Rincón Nayive Pino-Benítez Elkin Galeano Cris Rojo-Uribe Ana C. Mesa-Arango Oscar A. Flórez-Acosta |
| author_sort | Patricia Quintero-Rincón |
| collection | DOAJ |
| description | The Colombian Chocó is known for its rich biodiversity and to harbor plant species that are under-explored, including the genus <i>Sloanea</i>. This study aimed to analyze the chemical composition of derivatized ethanolic extracts from <i>S. chocoana</i> and <i>S. pittieriana</i> using BSTFA and TMCS through GC–MS, and to assess cell viability of immortalized human non-tumorigenic keratinocytes (HaCaT) and periodontal ligament fibroblast cells using crude extracts through MTS assay. Antioxidant and photoprotective properties were determined using DPPH assay and spectrophotometry. Antifungal activity of extracts against <i>Candida</i> species was developed following the CLSI standard M27, 4th ed. The sun protective factor (SPF) and UVA/UVB ratio values were calculated using the Mansur equation and the Boots star rating system. The critical wavelength (λc) was determined by calculating the integrated optical density curve’s area. The transmission of erythema and pigmentation was calculated through equations that use constants to calculate the flux of erythema and pigmentation. The GC–MS analysis identified 37 compounds for <i>S. chocoana</i> and 38 for <i>S. pittieriana</i>, including alkaloids, triterpenoids, and polyphenolics, among others. Both extracts exhibited proliferative effects on periodontal ligament fibroblasts, did not affect the viability of HaCaT cells, and showed excellent antioxidant activities (46.1% and 43.7%). Relevant antifungal activity was observed with <i>S. pittieriana</i> extract against <i>Candida albicans</i> (GM–MIC: 4 µg/mL), followed by <i>C. auris</i> and <i>C. glabrata</i> (GM–MIC: 32 µg/mL), while <i>S. chocoana</i> extract was active against <i>C. albicans</i> and <i>C. glabrata</i> (GM–MIC: 16 and 32 µg/mL, respectively). High SPF values (31.0 and 30.0), λc (393.98 and 337.81 nm), UVA/UVB ratio (1.5 and 1.2), and low percentage of transmission of erythema and pigmentation were determined for <i>S. chocoana</i> and <i>S. pittieriana</i>, respectively. Results showed that species of <i>Sloanea</i> constitute a promising alternative as ingredients for developing skincare products, and exhaustive studies are required for their sustainable uses. |
| first_indexed | 2024-03-09T01:45:28Z |
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| language | English |
| last_indexed | 2024-03-09T01:45:28Z |
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| spelling | doaj.art-eb9e54a03678461ca788bf06ad0723292023-12-08T15:23:45ZengMDPI AGPlants2223-77472023-11-011223395310.3390/plants12233953<i>Sloanea chocoana</i> and <i>S. pittieriana</i> (Elaeocarpaceae): Chemical and Biological Studies of Ethanolic Extracts and Skincare PropertiesPatricia Quintero-Rincón0Nayive Pino-Benítez1Elkin Galeano2Cris Rojo-Uribe3Ana C. Mesa-Arango4Oscar A. Flórez-Acosta5Natural Products Group, Universidad Tecnológica del Chocó, Quibdo 270002, ColombiaNatural Products Group, Universidad Tecnológica del Chocó, Quibdo 270002, ColombiaBioactive Substances Research Group, Faculty of Pharmaceutical and Food Sciences, Universidad de Antioquia, Medellin 050010, ColombiaDermatological Research Group, Faculty of Medicine, Universidad de Antioquia, Medellin 050010, ColombiaDermatological Research Group, Faculty of Medicine, Universidad de Antioquia, Medellin 050010, ColombiaResearch Group Design and Formulation of Medicines, Cosmetics, and Related, Faculty of Pharmaceutical and Food Sciences, Universidad de Antioquia, Medellin 050010, ColombiaThe Colombian Chocó is known for its rich biodiversity and to harbor plant species that are under-explored, including the genus <i>Sloanea</i>. This study aimed to analyze the chemical composition of derivatized ethanolic extracts from <i>S. chocoana</i> and <i>S. pittieriana</i> using BSTFA and TMCS through GC–MS, and to assess cell viability of immortalized human non-tumorigenic keratinocytes (HaCaT) and periodontal ligament fibroblast cells using crude extracts through MTS assay. Antioxidant and photoprotective properties were determined using DPPH assay and spectrophotometry. Antifungal activity of extracts against <i>Candida</i> species was developed following the CLSI standard M27, 4th ed. The sun protective factor (SPF) and UVA/UVB ratio values were calculated using the Mansur equation and the Boots star rating system. The critical wavelength (λc) was determined by calculating the integrated optical density curve’s area. The transmission of erythema and pigmentation was calculated through equations that use constants to calculate the flux of erythema and pigmentation. The GC–MS analysis identified 37 compounds for <i>S. chocoana</i> and 38 for <i>S. pittieriana</i>, including alkaloids, triterpenoids, and polyphenolics, among others. Both extracts exhibited proliferative effects on periodontal ligament fibroblasts, did not affect the viability of HaCaT cells, and showed excellent antioxidant activities (46.1% and 43.7%). Relevant antifungal activity was observed with <i>S. pittieriana</i> extract against <i>Candida albicans</i> (GM–MIC: 4 µg/mL), followed by <i>C. auris</i> and <i>C. glabrata</i> (GM–MIC: 32 µg/mL), while <i>S. chocoana</i> extract was active against <i>C. albicans</i> and <i>C. glabrata</i> (GM–MIC: 16 and 32 µg/mL, respectively). High SPF values (31.0 and 30.0), λc (393.98 and 337.81 nm), UVA/UVB ratio (1.5 and 1.2), and low percentage of transmission of erythema and pigmentation were determined for <i>S. chocoana</i> and <i>S. pittieriana</i>, respectively. Results showed that species of <i>Sloanea</i> constitute a promising alternative as ingredients for developing skincare products, and exhaustive studies are required for their sustainable uses.https://www.mdpi.com/2223-7747/12/23/3953biodiversity and sustainabilitybioprospectingtraditional medicinegenus <i>Sloanea</i>skincarehuman skin cells |
| spellingShingle | Patricia Quintero-Rincón Nayive Pino-Benítez Elkin Galeano Cris Rojo-Uribe Ana C. Mesa-Arango Oscar A. Flórez-Acosta <i>Sloanea chocoana</i> and <i>S. pittieriana</i> (Elaeocarpaceae): Chemical and Biological Studies of Ethanolic Extracts and Skincare Properties Plants biodiversity and sustainability bioprospecting traditional medicine genus <i>Sloanea</i> skincare human skin cells |
| title | <i>Sloanea chocoana</i> and <i>S. pittieriana</i> (Elaeocarpaceae): Chemical and Biological Studies of Ethanolic Extracts and Skincare Properties |
| title_full | <i>Sloanea chocoana</i> and <i>S. pittieriana</i> (Elaeocarpaceae): Chemical and Biological Studies of Ethanolic Extracts and Skincare Properties |
| title_fullStr | <i>Sloanea chocoana</i> and <i>S. pittieriana</i> (Elaeocarpaceae): Chemical and Biological Studies of Ethanolic Extracts and Skincare Properties |
| title_full_unstemmed | <i>Sloanea chocoana</i> and <i>S. pittieriana</i> (Elaeocarpaceae): Chemical and Biological Studies of Ethanolic Extracts and Skincare Properties |
| title_short | <i>Sloanea chocoana</i> and <i>S. pittieriana</i> (Elaeocarpaceae): Chemical and Biological Studies of Ethanolic Extracts and Skincare Properties |
| title_sort | i sloanea chocoana i and i s pittieriana i elaeocarpaceae chemical and biological studies of ethanolic extracts and skincare properties |
| topic | biodiversity and sustainability bioprospecting traditional medicine genus <i>Sloanea</i> skincare human skin cells |
| url | https://www.mdpi.com/2223-7747/12/23/3953 |
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