Fabrication of low-cost micropatterned polydimethyl-siloxane scaffolds to organise cells in a variety of two-dimensioanl biomimetic arrangements for lab-on-chip culture platforms
We present the rapid-prototyping of type I collagen micropatterns on poly-dimethylsiloxane substrates for the biomimetic confinement of cells using the combination of a surface oxidation treatment and 3-aminopropyl triethoxysilane silanisation followed by glutaraldehyde crosslinking. The aim of surf...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2017-11-01
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| Series: | Journal of Tissue Engineering |
| Online Access: | https://doi.org/10.1177/2041731417741505 |
| _version_ | 1818509308427501568 |
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| author | Lidia Escutia-Guadarrama Genaro Vázquez-Victorio David Martínez-Pastor Brenda Nieto-Rivera Marcela Sosa-Garrocho Marina Macías-Silva Mathieu Hautefeuille |
| author_facet | Lidia Escutia-Guadarrama Genaro Vázquez-Victorio David Martínez-Pastor Brenda Nieto-Rivera Marcela Sosa-Garrocho Marina Macías-Silva Mathieu Hautefeuille |
| author_sort | Lidia Escutia-Guadarrama |
| collection | DOAJ |
| description | We present the rapid-prototyping of type I collagen micropatterns on poly-dimethylsiloxane substrates for the biomimetic confinement of cells using the combination of a surface oxidation treatment and 3-aminopropyl triethoxysilane silanisation followed by glutaraldehyde crosslinking. The aim of surface treatment is to stabilise microcontact printing transfer of this natural extracellular matrix protein that usually wears out easily from poly-dimethylsiloxane, which is not suitable for biomimetic cell culture platforms and lab-on-chip applications. A low-cost CD-DVD laser was used to etch biomimetic micropatterns into acrylic sheets that were in turn replicated to poly-dimethylsiloxane slabs with the desired features. These stamps were finally inked with type I collagen for microcontact printing transfer on the culture substrates in a simple manner. Human hepatoma cells (HepG2) and rat primary hepatocytes, which do not adhere to bare poly-dimethylsiloxane, were successfully seeded and showed optimal adhesion and survival on simple protein micropatterns with a hepatic cord geometry in order to validate our technique. HepG2 cells also proliferated on the stamps. Soft and stiff poly-dimethylsiloxane layers were also tested to demonstrate that our cost-effective process is compatible with biomimetic organ-on-chip technology integrating tunable stiffness with a potential application to drug testing probes development where such cells are commonly used. |
| first_indexed | 2024-12-10T22:43:49Z |
| format | Article |
| id | doaj.art-eb9eec21b00e44e486e8d38b8407f42d |
| institution | Directory Open Access Journal |
| issn | 2041-7314 |
| language | English |
| last_indexed | 2024-12-10T22:43:49Z |
| publishDate | 2017-11-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Journal of Tissue Engineering |
| spelling | doaj.art-eb9eec21b00e44e486e8d38b8407f42d2022-12-22T01:30:39ZengSAGE PublishingJournal of Tissue Engineering2041-73142017-11-01810.1177/2041731417741505Fabrication of low-cost micropatterned polydimethyl-siloxane scaffolds to organise cells in a variety of two-dimensioanl biomimetic arrangements for lab-on-chip culture platformsLidia Escutia-Guadarrama0Genaro Vázquez-Victorio1David Martínez-Pastor2Brenda Nieto-Rivera3Marcela Sosa-Garrocho4Marina Macías-Silva5Mathieu Hautefeuille6Laboratorio Nacional de Soluciones Biomiméticas para Diagnóstico y Terapia, Facultad de Ciencias, Universidad Nacional Autónoma de México, Ciudad de México, MéxicoLaboratorio Nacional de Soluciones Biomiméticas para Diagnóstico y Terapia, Facultad de Ciencias, Universidad Nacional Autónoma de México, Ciudad de México, MéxicoInstituto de Fisiología Celular, Universidad Nacional Autónoma de México, Ciudad de México, MéxicoFacultad de Ciencias, Universidad Nacional Autónoma de México, Ciudad de México, MéxicoInstituto de Fisiología Celular, Universidad Nacional Autónoma de México, Ciudad de México, MéxicoLaboratorio Nacional de Soluciones Biomiméticas para Diagnóstico y Terapia, Facultad de Ciencias, Universidad Nacional Autónoma de México, Ciudad de México, MéxicoLaboratorio Nacional de Soluciones Biomiméticas para Diagnóstico y Terapia, Facultad de Ciencias, Universidad Nacional Autónoma de México, Ciudad de México, MéxicoWe present the rapid-prototyping of type I collagen micropatterns on poly-dimethylsiloxane substrates for the biomimetic confinement of cells using the combination of a surface oxidation treatment and 3-aminopropyl triethoxysilane silanisation followed by glutaraldehyde crosslinking. The aim of surface treatment is to stabilise microcontact printing transfer of this natural extracellular matrix protein that usually wears out easily from poly-dimethylsiloxane, which is not suitable for biomimetic cell culture platforms and lab-on-chip applications. A low-cost CD-DVD laser was used to etch biomimetic micropatterns into acrylic sheets that were in turn replicated to poly-dimethylsiloxane slabs with the desired features. These stamps were finally inked with type I collagen for microcontact printing transfer on the culture substrates in a simple manner. Human hepatoma cells (HepG2) and rat primary hepatocytes, which do not adhere to bare poly-dimethylsiloxane, were successfully seeded and showed optimal adhesion and survival on simple protein micropatterns with a hepatic cord geometry in order to validate our technique. HepG2 cells also proliferated on the stamps. Soft and stiff poly-dimethylsiloxane layers were also tested to demonstrate that our cost-effective process is compatible with biomimetic organ-on-chip technology integrating tunable stiffness with a potential application to drug testing probes development where such cells are commonly used.https://doi.org/10.1177/2041731417741505 |
| spellingShingle | Lidia Escutia-Guadarrama Genaro Vázquez-Victorio David Martínez-Pastor Brenda Nieto-Rivera Marcela Sosa-Garrocho Marina Macías-Silva Mathieu Hautefeuille Fabrication of low-cost micropatterned polydimethyl-siloxane scaffolds to organise cells in a variety of two-dimensioanl biomimetic arrangements for lab-on-chip culture platforms Journal of Tissue Engineering |
| title | Fabrication of low-cost micropatterned polydimethyl-siloxane scaffolds to organise cells in a variety of two-dimensioanl biomimetic arrangements for lab-on-chip culture platforms |
| title_full | Fabrication of low-cost micropatterned polydimethyl-siloxane scaffolds to organise cells in a variety of two-dimensioanl biomimetic arrangements for lab-on-chip culture platforms |
| title_fullStr | Fabrication of low-cost micropatterned polydimethyl-siloxane scaffolds to organise cells in a variety of two-dimensioanl biomimetic arrangements for lab-on-chip culture platforms |
| title_full_unstemmed | Fabrication of low-cost micropatterned polydimethyl-siloxane scaffolds to organise cells in a variety of two-dimensioanl biomimetic arrangements for lab-on-chip culture platforms |
| title_short | Fabrication of low-cost micropatterned polydimethyl-siloxane scaffolds to organise cells in a variety of two-dimensioanl biomimetic arrangements for lab-on-chip culture platforms |
| title_sort | fabrication of low cost micropatterned polydimethyl siloxane scaffolds to organise cells in a variety of two dimensioanl biomimetic arrangements for lab on chip culture platforms |
| url | https://doi.org/10.1177/2041731417741505 |
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