The Effect of Temperature on the Stability of African Swine Fever Virus BA71V Isolate in Environmental Water Samples
African swine fever virus (ASFV) is known to be very stable and can remain infectious over long periods of time especially at low temperatures and within different matrices, particularly those containing animal-derived organic material. However, there are some gaps in our knowledge pertaining to the...
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| Format: | Article |
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MDPI AG
2023-08-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/12/8/1022 |
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| author | Eleni-Anna Loundras Christopher L. Netherton John Flannery Michael J. Bowes Linda Dixon Carrie Batten |
| author_facet | Eleni-Anna Loundras Christopher L. Netherton John Flannery Michael J. Bowes Linda Dixon Carrie Batten |
| author_sort | Eleni-Anna Loundras |
| collection | DOAJ |
| description | African swine fever virus (ASFV) is known to be very stable and can remain infectious over long periods of time especially at low temperatures and within different matrices, particularly those containing animal-derived organic material. However, there are some gaps in our knowledge pertaining to the survivability and infectivity of ASFV in groundwater. This study aims to determine the stability and infectivity of the cell culture-adapted ASFV strain BA71V by plaque assay after incubation of the virus within river water samples at three different environmentally relevant temperatures (4 °C, 15 °C, and 21 °C) over the course of 42 days. The results from this study indicate that ASFV can remain stable and infectious when maintained at 4 °C in river water for more than 42 days, but as incubation temperatures are increased, the stability is reduced, and the virus is no longer able to form plaques after 28 days and 14 days, respectively, when stored at 15 °C and 21 °C. Characterizing the survivability of ASFV in groundwater can allow us to develop more appropriate inactivation and disinfection methods to support disease control and mitigate ASFV outbreaks. |
| first_indexed | 2024-03-10T23:41:01Z |
| format | Article |
| id | doaj.art-eb9fa6610f7143d6b7d92c70fc19fadc |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-10T23:41:01Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
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| series | Pathogens |
| spelling | doaj.art-eb9fa6610f7143d6b7d92c70fc19fadc2023-11-19T02:32:23ZengMDPI AGPathogens2076-08172023-08-01128102210.3390/pathogens12081022The Effect of Temperature on the Stability of African Swine Fever Virus BA71V Isolate in Environmental Water SamplesEleni-Anna Loundras0Christopher L. Netherton1John Flannery2Michael J. Bowes3Linda Dixon4Carrie Batten5The Pirbright Institute, Ash Road, Pirbright, Woking GU24 0NF, UKThe Pirbright Institute, Ash Road, Pirbright, Woking GU24 0NF, UKThe Pirbright Institute, Ash Road, Pirbright, Woking GU24 0NF, UKCentre for Ecology and Hydrology, Wallingford Oxon OX10 8BB, UKThe Pirbright Institute, Ash Road, Pirbright, Woking GU24 0NF, UKThe Pirbright Institute, Ash Road, Pirbright, Woking GU24 0NF, UKAfrican swine fever virus (ASFV) is known to be very stable and can remain infectious over long periods of time especially at low temperatures and within different matrices, particularly those containing animal-derived organic material. However, there are some gaps in our knowledge pertaining to the survivability and infectivity of ASFV in groundwater. This study aims to determine the stability and infectivity of the cell culture-adapted ASFV strain BA71V by plaque assay after incubation of the virus within river water samples at three different environmentally relevant temperatures (4 °C, 15 °C, and 21 °C) over the course of 42 days. The results from this study indicate that ASFV can remain stable and infectious when maintained at 4 °C in river water for more than 42 days, but as incubation temperatures are increased, the stability is reduced, and the virus is no longer able to form plaques after 28 days and 14 days, respectively, when stored at 15 °C and 21 °C. Characterizing the survivability of ASFV in groundwater can allow us to develop more appropriate inactivation and disinfection methods to support disease control and mitigate ASFV outbreaks.https://www.mdpi.com/2076-0817/12/8/1022African swine fever virusstabilityinfectivityenvironmental watergroundwater |
| spellingShingle | Eleni-Anna Loundras Christopher L. Netherton John Flannery Michael J. Bowes Linda Dixon Carrie Batten The Effect of Temperature on the Stability of African Swine Fever Virus BA71V Isolate in Environmental Water Samples Pathogens African swine fever virus stability infectivity environmental water groundwater |
| title | The Effect of Temperature on the Stability of African Swine Fever Virus BA71V Isolate in Environmental Water Samples |
| title_full | The Effect of Temperature on the Stability of African Swine Fever Virus BA71V Isolate in Environmental Water Samples |
| title_fullStr | The Effect of Temperature on the Stability of African Swine Fever Virus BA71V Isolate in Environmental Water Samples |
| title_full_unstemmed | The Effect of Temperature on the Stability of African Swine Fever Virus BA71V Isolate in Environmental Water Samples |
| title_short | The Effect of Temperature on the Stability of African Swine Fever Virus BA71V Isolate in Environmental Water Samples |
| title_sort | effect of temperature on the stability of african swine fever virus ba71v isolate in environmental water samples |
| topic | African swine fever virus stability infectivity environmental water groundwater |
| url | https://www.mdpi.com/2076-0817/12/8/1022 |
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