Effects and Stress-Relieving Mechanisms of Dark Tea Polysaccharide in Human HaCaT Keratinocytes and SZ95 Sebocytes
A new skincare application scenario for dark tea, a unique and post-fermented tea popular in the health food industry, was developed in this paper. The effects of dark tea polysaccharide (DTP) on stress-induced skin problems and its mechanism of action were investigated by modeling cortisone-induced...
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MDPI AG
2023-08-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/28/16/6128 |
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| author | Chang Gao Jiafeng Fu Junyi Cui Tingzhi Zhang Christos C. Zouboulis Jing Wang Shaowei Yan |
| author_facet | Chang Gao Jiafeng Fu Junyi Cui Tingzhi Zhang Christos C. Zouboulis Jing Wang Shaowei Yan |
| author_sort | Chang Gao |
| collection | DOAJ |
| description | A new skincare application scenario for dark tea, a unique and post-fermented tea popular in the health food industry, was developed in this paper. The effects of dark tea polysaccharide (DTP) on stress-induced skin problems and its mechanism of action were investigated by modeling cortisone-induced stress injury in human HaCaT keratinocytes and SZ95 sebaceous gland cells. The results showed a reduced cortisol conversion induced by cortisone under the action of DTP with a concentration of 200 μg/mL, probably by inhibiting the expression of the HSD11B1 enzyme. DTP was also able to suppress the cortisone-induced elevation of lipid levels in SZ95 sebocytes at this concentration. In addition, the composition and structure of DTP were verified by ultrafiltration, ultraviolet-visible spectrophotometry (UV-VIS), high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) and infrared spectroscopy. In brief, DTP has a unique and significant stress-relieving effect, which provides new ideas for the development of new ingredients for the skin care industry. |
| first_indexed | 2024-03-10T23:42:03Z |
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| id | doaj.art-eba25db5f86343f3aca9c1c3ec9cd287 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T23:42:03Z |
| publishDate | 2023-08-01 |
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| series | Molecules |
| spelling | doaj.art-eba25db5f86343f3aca9c1c3ec9cd2872023-11-19T02:25:20ZengMDPI AGMolecules1420-30492023-08-012816612810.3390/molecules28166128Effects and Stress-Relieving Mechanisms of Dark Tea Polysaccharide in Human HaCaT Keratinocytes and SZ95 SebocytesChang Gao0Jiafeng Fu1Junyi Cui2Tingzhi Zhang3Christos C. Zouboulis4Jing Wang5Shaowei Yan6Syoung Cosmetics Manufacturing Co., Ltd., Changsha 410000, ChinaSyoung Cosmetics Manufacturing Co., Ltd., Changsha 410000, ChinaSyoung Cosmetics Manufacturing Co., Ltd., Changsha 410000, ChinaSyoung Cosmetics Manufacturing Co., Ltd., Changsha 410000, ChinaDepartments of Dermatology, Venereology, Allergology and Immunology, Staedtisches Klinikum Dessau, Brandenburg Medical School Theodor Fontane, Faculty of Health Sciences Brandenburg, Auenweg 38, 06847 Dessau, GermanySchool of Chemistry and Material Engineering, Jiangnan University, Wuxi 214122, ChinaSyoung Cosmetics Manufacturing Co., Ltd., Changsha 410000, ChinaA new skincare application scenario for dark tea, a unique and post-fermented tea popular in the health food industry, was developed in this paper. The effects of dark tea polysaccharide (DTP) on stress-induced skin problems and its mechanism of action were investigated by modeling cortisone-induced stress injury in human HaCaT keratinocytes and SZ95 sebaceous gland cells. The results showed a reduced cortisol conversion induced by cortisone under the action of DTP with a concentration of 200 μg/mL, probably by inhibiting the expression of the HSD11B1 enzyme. DTP was also able to suppress the cortisone-induced elevation of lipid levels in SZ95 sebocytes at this concentration. In addition, the composition and structure of DTP were verified by ultrafiltration, ultraviolet-visible spectrophotometry (UV-VIS), high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) and infrared spectroscopy. In brief, DTP has a unique and significant stress-relieving effect, which provides new ideas for the development of new ingredients for the skin care industry.https://www.mdpi.com/1420-3049/28/16/6128stressdark tea polysaccharidecortisolsebum |
| spellingShingle | Chang Gao Jiafeng Fu Junyi Cui Tingzhi Zhang Christos C. Zouboulis Jing Wang Shaowei Yan Effects and Stress-Relieving Mechanisms of Dark Tea Polysaccharide in Human HaCaT Keratinocytes and SZ95 Sebocytes Molecules stress dark tea polysaccharide cortisol sebum |
| title | Effects and Stress-Relieving Mechanisms of Dark Tea Polysaccharide in Human HaCaT Keratinocytes and SZ95 Sebocytes |
| title_full | Effects and Stress-Relieving Mechanisms of Dark Tea Polysaccharide in Human HaCaT Keratinocytes and SZ95 Sebocytes |
| title_fullStr | Effects and Stress-Relieving Mechanisms of Dark Tea Polysaccharide in Human HaCaT Keratinocytes and SZ95 Sebocytes |
| title_full_unstemmed | Effects and Stress-Relieving Mechanisms of Dark Tea Polysaccharide in Human HaCaT Keratinocytes and SZ95 Sebocytes |
| title_short | Effects and Stress-Relieving Mechanisms of Dark Tea Polysaccharide in Human HaCaT Keratinocytes and SZ95 Sebocytes |
| title_sort | effects and stress relieving mechanisms of dark tea polysaccharide in human hacat keratinocytes and sz95 sebocytes |
| topic | stress dark tea polysaccharide cortisol sebum |
| url | https://www.mdpi.com/1420-3049/28/16/6128 |
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