Regenerative Potential of Blood-Derived Products in 3D Osteoarthritic Chondrocyte Culture System
Intra-articular injection of different types of blood-derived products is gaining popularity and clinical importance in the treatment of degenerative cartilage disorders such as osteoarthritis. The regenerative potential of two types of platelet-rich plasma (PRP), prepared in the presence of EDTA (E...
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| Format: | Article |
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MDPI AG
2021-07-01
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| Series: | Current Issues in Molecular Biology |
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| Online Access: | https://www.mdpi.com/1467-3045/43/2/48 |
| _version_ | 1797519800417648640 |
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| author | Olga Kuten-Pella Andrea De Luna Karina Kramer Markus Neubauer Stefan Nehrer Zsombor Lacza |
| author_facet | Olga Kuten-Pella Andrea De Luna Karina Kramer Markus Neubauer Stefan Nehrer Zsombor Lacza |
| author_sort | Olga Kuten-Pella |
| collection | DOAJ |
| description | Intra-articular injection of different types of blood-derived products is gaining popularity and clinical importance in the treatment of degenerative cartilage disorders such as osteoarthritis. The regenerative potential of two types of platelet-rich plasma (PRP), prepared in the presence of EDTA (EPRP) and citrate (CPRP) and an alternative blood product-hyperacute serum (hypACT) was evaluated using a 3D osteoarthritic chondrocyte pellet model by assessing the metabolic cell activity, cartilage-related gene expression and extracellular matrix deposition within the pellets. Chondrocyte viability was determined by XTT assay and it revealed no significant difference in metabolic activity of OA chondrocyte pellets after supplementation with different blood products. Nevertheless, the selection of blood products influenced the cartilage-related genes expression, ECM morphology and the tissue quality of pellets. Both PRP types had a different biological effect depending upon concentration and even though CPRP is widely used in clinics our assessment did not reveal good results in gene expression either tissue quality. HypACT supplementation resulted in superior cartilage-related genes expression together with tissue quality and seemed to be the most stable product since no remarkable changes were observed between the two different concentrations. All in all, for successful regenerative therapy, possible molecular mechanisms induced by blood-derived products should be always carefully investigated and adapted to the specific medical indications. |
| first_indexed | 2024-03-10T07:47:55Z |
| format | Article |
| id | doaj.art-eba3f1e6e78342efb3a3e28a681016db |
| institution | Directory Open Access Journal |
| issn | 1467-3037 1467-3045 |
| language | English |
| last_indexed | 2024-03-10T07:47:55Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Current Issues in Molecular Biology |
| spelling | doaj.art-eba3f1e6e78342efb3a3e28a681016db2023-11-22T12:30:57ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452021-07-0143266567510.3390/cimb43020048Regenerative Potential of Blood-Derived Products in 3D Osteoarthritic Chondrocyte Culture SystemOlga Kuten-Pella0Andrea De Luna1Karina Kramer2Markus Neubauer3Stefan Nehrer4Zsombor Lacza5OrthoSera GmbH, 3500 Krems, AustriaCenter for Regenerative Medicine, Danube University Krems, 3500 Krems, AustriaCenter for Regenerative Medicine, Danube University Krems, 3500 Krems, AustriaCenter for Regenerative Medicine, Danube University Krems, 3500 Krems, AustriaCenter for Regenerative Medicine, Danube University Krems, 3500 Krems, AustriaOrthoSera GmbH, 3500 Krems, AustriaIntra-articular injection of different types of blood-derived products is gaining popularity and clinical importance in the treatment of degenerative cartilage disorders such as osteoarthritis. The regenerative potential of two types of platelet-rich plasma (PRP), prepared in the presence of EDTA (EPRP) and citrate (CPRP) and an alternative blood product-hyperacute serum (hypACT) was evaluated using a 3D osteoarthritic chondrocyte pellet model by assessing the metabolic cell activity, cartilage-related gene expression and extracellular matrix deposition within the pellets. Chondrocyte viability was determined by XTT assay and it revealed no significant difference in metabolic activity of OA chondrocyte pellets after supplementation with different blood products. Nevertheless, the selection of blood products influenced the cartilage-related genes expression, ECM morphology and the tissue quality of pellets. Both PRP types had a different biological effect depending upon concentration and even though CPRP is widely used in clinics our assessment did not reveal good results in gene expression either tissue quality. HypACT supplementation resulted in superior cartilage-related genes expression together with tissue quality and seemed to be the most stable product since no remarkable changes were observed between the two different concentrations. All in all, for successful regenerative therapy, possible molecular mechanisms induced by blood-derived products should be always carefully investigated and adapted to the specific medical indications.https://www.mdpi.com/1467-3045/43/2/48osteoarthritic chondrocytescartilage3D pellet cultureblood productsplatelet-rich plasmahyperacute serum |
| spellingShingle | Olga Kuten-Pella Andrea De Luna Karina Kramer Markus Neubauer Stefan Nehrer Zsombor Lacza Regenerative Potential of Blood-Derived Products in 3D Osteoarthritic Chondrocyte Culture System Current Issues in Molecular Biology osteoarthritic chondrocytes cartilage 3D pellet culture blood products platelet-rich plasma hyperacute serum |
| title | Regenerative Potential of Blood-Derived Products in 3D Osteoarthritic Chondrocyte Culture System |
| title_full | Regenerative Potential of Blood-Derived Products in 3D Osteoarthritic Chondrocyte Culture System |
| title_fullStr | Regenerative Potential of Blood-Derived Products in 3D Osteoarthritic Chondrocyte Culture System |
| title_full_unstemmed | Regenerative Potential of Blood-Derived Products in 3D Osteoarthritic Chondrocyte Culture System |
| title_short | Regenerative Potential of Blood-Derived Products in 3D Osteoarthritic Chondrocyte Culture System |
| title_sort | regenerative potential of blood derived products in 3d osteoarthritic chondrocyte culture system |
| topic | osteoarthritic chondrocytes cartilage 3D pellet culture blood products platelet-rich plasma hyperacute serum |
| url | https://www.mdpi.com/1467-3045/43/2/48 |
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