Monoxenic rearing of Ditylenchus weischeri and D. dipsaci and microplot examination of the host suitability of yellow pea to D. weischeri
Ditylenchus weischeri was recently reported in the provinces of Manitoba and Saskatchewan, Canada. Populations of D. weischeri from creeping thistle (Cirsium arvense L.) in Manitoba and D. dipsaci from garlic (Allium cepa L.) in Ontario were examined for their potential to grow on callused carrot (D...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2017-12-01
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| Series: | Plant Protection Science |
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| Online Access: | https://pps.agriculturejournals.cz/artkey/pps-201704-0010_monoxenic-rearing-of-ditylenchus-weischeri-and-d-dipsaci-and-microplot-examination-of-the-host-suitability-of.php |
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| author | Abolfazl Hajihassani Mario Tenuta Robert H. Gulden |
| author_facet | Abolfazl Hajihassani Mario Tenuta Robert H. Gulden |
| author_sort | Abolfazl Hajihassani |
| collection | DOAJ |
| description | Ditylenchus weischeri was recently reported in the provinces of Manitoba and Saskatchewan, Canada. Populations of D. weischeri from creeping thistle (Cirsium arvense L.) in Manitoba and D. dipsaci from garlic (Allium cepa L.) in Ontario were examined for their potential to grow on callused carrot (Daucus carota subsp. sativus) disks, alfalfa (Medi- cago sativa L.) and creeping thistle callus tissues, and pure cultures of eight fungal species, Botrytis cinerea, Fusarium solani, Rhizoctonia solani, Verticillium dahliae, Sclerotinia sclerotiorum, Cladosporium cucumerinum, Colletotrichum gloeosporioides, and Chaetomium spp. Ditylenchus weischeri and D. dipsaci could not be reared on any of the fungal isolates nor in the callus tissues of creeping thistle. In contrast to D. weischeri, D. dipsaci was successfully reared on the alfalfa callus tissue. On the callused carrot disks, with no media, an increase of 54 and 244 times the initial density of 80 nematodes was obtained for D. weischeri and D. dipsaci, respectively. Monoxenic rearing was performed using callused carrot disks to provide sufficient D. weischeri inoculum for the microplot study. The effect of D. weischeri on yellow pea varieties Agassiz and Bronco was determined in a microplot trial using initial densities of 0, 100, 200, 400, 800, 1600, and 3200 nematodes/plant. While it had no impact on pea grain yield, D. weischeri slightly reduced plant height, aboveground biomass, and pod length at the population densities of 1600 and 3200 nematodes/plant. The final population densities at harvest were not significantly different from the initial densities indicating the pea varieties were poor hosts to D. weischeri. The results of the present study indicate that D. weischeri is unlikely to be a pest of yellow pea for weather conditions of the Canadian Prairies. |
| first_indexed | 2024-04-10T08:15:43Z |
| format | Article |
| id | doaj.art-eba429d337d44f37ab9ace9d35517347 |
| institution | Directory Open Access Journal |
| issn | 1212-2580 1805-9341 |
| language | English |
| last_indexed | 2024-04-10T08:15:43Z |
| publishDate | 2017-12-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Plant Protection Science |
| spelling | doaj.art-eba429d337d44f37ab9ace9d355173472023-02-23T03:44:20ZengCzech Academy of Agricultural SciencesPlant Protection Science1212-25801805-93412017-12-0153425426410.17221/158/2016-PPSpps-201704-0010Monoxenic rearing of Ditylenchus weischeri and D. dipsaci and microplot examination of the host suitability of yellow pea to D. weischeriAbolfazl Hajihassani0Mario Tenuta1Robert H. Gulden2Department of Soil Science andDepartment of Soil Science andDepartment of Plant Science, University of Manitoba, Winnipeg, Manitoba, CanadaDitylenchus weischeri was recently reported in the provinces of Manitoba and Saskatchewan, Canada. Populations of D. weischeri from creeping thistle (Cirsium arvense L.) in Manitoba and D. dipsaci from garlic (Allium cepa L.) in Ontario were examined for their potential to grow on callused carrot (Daucus carota subsp. sativus) disks, alfalfa (Medi- cago sativa L.) and creeping thistle callus tissues, and pure cultures of eight fungal species, Botrytis cinerea, Fusarium solani, Rhizoctonia solani, Verticillium dahliae, Sclerotinia sclerotiorum, Cladosporium cucumerinum, Colletotrichum gloeosporioides, and Chaetomium spp. Ditylenchus weischeri and D. dipsaci could not be reared on any of the fungal isolates nor in the callus tissues of creeping thistle. In contrast to D. weischeri, D. dipsaci was successfully reared on the alfalfa callus tissue. On the callused carrot disks, with no media, an increase of 54 and 244 times the initial density of 80 nematodes was obtained for D. weischeri and D. dipsaci, respectively. Monoxenic rearing was performed using callused carrot disks to provide sufficient D. weischeri inoculum for the microplot study. The effect of D. weischeri on yellow pea varieties Agassiz and Bronco was determined in a microplot trial using initial densities of 0, 100, 200, 400, 800, 1600, and 3200 nematodes/plant. While it had no impact on pea grain yield, D. weischeri slightly reduced plant height, aboveground biomass, and pod length at the population densities of 1600 and 3200 nematodes/plant. The final population densities at harvest were not significantly different from the initial densities indicating the pea varieties were poor hosts to D. weischeri. The results of the present study indicate that D. weischeri is unlikely to be a pest of yellow pea for weather conditions of the Canadian Prairies.https://pps.agriculturejournals.cz/artkey/pps-201704-0010_monoxenic-rearing-of-ditylenchus-weischeri-and-d-dipsaci-and-microplot-examination-of-the-host-suitability-of.phpcallused carrot diskstem nematodehostplotyield |
| spellingShingle | Abolfazl Hajihassani Mario Tenuta Robert H. Gulden Monoxenic rearing of Ditylenchus weischeri and D. dipsaci and microplot examination of the host suitability of yellow pea to D. weischeri Plant Protection Science callused carrot disk stem nematode host plot yield |
| title | Monoxenic rearing of Ditylenchus weischeri and D. dipsaci and microplot examination of the host suitability of yellow pea to D. weischeri |
| title_full | Monoxenic rearing of Ditylenchus weischeri and D. dipsaci and microplot examination of the host suitability of yellow pea to D. weischeri |
| title_fullStr | Monoxenic rearing of Ditylenchus weischeri and D. dipsaci and microplot examination of the host suitability of yellow pea to D. weischeri |
| title_full_unstemmed | Monoxenic rearing of Ditylenchus weischeri and D. dipsaci and microplot examination of the host suitability of yellow pea to D. weischeri |
| title_short | Monoxenic rearing of Ditylenchus weischeri and D. dipsaci and microplot examination of the host suitability of yellow pea to D. weischeri |
| title_sort | monoxenic rearing of ditylenchus weischeri and d dipsaci and microplot examination of the host suitability of yellow pea to d weischeri |
| topic | callused carrot disk stem nematode host plot yield |
| url | https://pps.agriculturejournals.cz/artkey/pps-201704-0010_monoxenic-rearing-of-ditylenchus-weischeri-and-d-dipsaci-and-microplot-examination-of-the-host-suitability-of.php |
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