Oral Administration of <i>Lactobacillus sakei</i> CVL-001 Improves Recovery from Dextran Sulfate Sodium-Induced Colitis in Mice by Microbiota Modulation
Inflammatory bowel disease (IBD) is an intestinal chronic inflammatory disease, and its incidence is steadily increasing. IBD is closely related to the intestinal microbiota, and probiotics are known to be a potential therapeutic agent for IBD. In our study, we evaluated the protective effect of <...
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2023-05-01
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author | Dong-Yeon Kim Tae-Sung Lee Do-Hyeon Jung Eun-Jung Song Ah-Ra Jang Ji-Yeon Park Jae-Hun Ahn In-Su Seo Seung-Ju Song Yeong-Jun Kim Yun-Ji Lee Yeon-Ji Lee Jong-Hwan Park |
author_facet | Dong-Yeon Kim Tae-Sung Lee Do-Hyeon Jung Eun-Jung Song Ah-Ra Jang Ji-Yeon Park Jae-Hun Ahn In-Su Seo Seung-Ju Song Yeong-Jun Kim Yun-Ji Lee Yeon-Ji Lee Jong-Hwan Park |
author_sort | Dong-Yeon Kim |
collection | DOAJ |
description | Inflammatory bowel disease (IBD) is an intestinal chronic inflammatory disease, and its incidence is steadily increasing. IBD is closely related to the intestinal microbiota, and probiotics are known to be a potential therapeutic agent for IBD. In our study, we evaluated the protective effect of <i>Lactobacillus sakei</i> CVL-001, isolated from <i>Baechu</i> kimchi, on dextran sulfated sodium (DSS)-induced colitis in mice. The oral administration of <i>L. sakei</i> CVL-001 according to the experimental schedule alleviated weight loss and disease activity in the mice with colitis. Furthermore, the length and histopathology of the colon improved. The expression of tumor necrosis factor (<i>TNF</i>)<i>-α</i> and interleukin <i>(IL)-1β</i> genes decreased in the colons of mice that were administered <i>L. sakei</i> CVL-001, whereas that of <i>IL-10</i> increased. The expressions of genes coding for E-cadherin, claudin3, occludin, and mucin were also restored. In co-housed conditions, <i>L. sakei</i> CVL-001 administration did not improve disease activity, colon length, and histopathology. Microbiota analysis revealed that <i>L. sakei</i> CVL-001 administration increased the abundance of microbiota and altered <i>Firmicutes</i>/<i>Bacteroidetes</i> ratio, and decreased <i>Proteobacteria</i>. In conclusion, <i>L. sakei</i> CVL-001 administration protects mice from DSS-induced colitis by regulating immune response and intestinal integrity via gut microbiota modulation. |
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language | English |
last_indexed | 2024-03-11T03:28:53Z |
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spelling | doaj.art-ebaab14a69e74f82b2b39b3f379d15022023-11-18T02:35:37ZengMDPI AGMicroorganisms2076-26072023-05-01115135910.3390/microorganisms11051359Oral Administration of <i>Lactobacillus sakei</i> CVL-001 Improves Recovery from Dextran Sulfate Sodium-Induced Colitis in Mice by Microbiota ModulationDong-Yeon Kim0Tae-Sung Lee1Do-Hyeon Jung2Eun-Jung Song3Ah-Ra Jang4Ji-Yeon Park5Jae-Hun Ahn6In-Su Seo7Seung-Ju Song8Yeong-Jun Kim9Yun-Ji Lee10Yeon-Ji Lee11Jong-Hwan Park12Laboratory Animal Medicine, Animal Medical Institute, College of Veterinary Medicine, Chonnam National University, 77 Yongbong-ro, Buk-gu, Gwangju 61186, Republic of KoreaLaboratory Animal Medicine, Animal Medical Institute, College of Veterinary Medicine, Chonnam National University, 77 Yongbong-ro, Buk-gu, Gwangju 61186, Republic of KoreaLaboratory Animal Medicine, Animal Medical Institute, College of Veterinary Medicine, Chonnam National University, 77 Yongbong-ro, Buk-gu, Gwangju 61186, Republic of KoreaNodcure, Inc., 77 Yongbong-ro, Buk-gu, Gwangju 61186, Republic of KoreaNodcure, Inc., 77 Yongbong-ro, Buk-gu, Gwangju 61186, Republic of KoreaLaboratory Animal Medicine, Animal Medical Institute, College of Veterinary Medicine, Chonnam National University, 77 Yongbong-ro, Buk-gu, Gwangju 61186, Republic of KoreaLaboratory Animal Medicine, Animal Medical Institute, College of Veterinary Medicine, Chonnam National University, 77 Yongbong-ro, Buk-gu, Gwangju 61186, Republic of KoreaLaboratory Animal Medicine, Animal Medical Institute, College of Veterinary Medicine, Chonnam National University, 77 Yongbong-ro, Buk-gu, Gwangju 61186, Republic of KoreaLaboratory Animal Medicine, Animal Medical Institute, College of Veterinary Medicine, Chonnam National University, 77 Yongbong-ro, Buk-gu, Gwangju 61186, Republic of KoreaLaboratory Animal Medicine, Animal Medical Institute, College of Veterinary Medicine, Chonnam National University, 77 Yongbong-ro, Buk-gu, Gwangju 61186, Republic of KoreaLaboratory Animal Medicine, Animal Medical Institute, College of Veterinary Medicine, Chonnam National University, 77 Yongbong-ro, Buk-gu, Gwangju 61186, Republic of KoreaLaboratory Animal Medicine, Animal Medical Institute, College of Veterinary Medicine, Chonnam National University, 77 Yongbong-ro, Buk-gu, Gwangju 61186, Republic of KoreaLaboratory Animal Medicine, Animal Medical Institute, College of Veterinary Medicine, Chonnam National University, 77 Yongbong-ro, Buk-gu, Gwangju 61186, Republic of KoreaInflammatory bowel disease (IBD) is an intestinal chronic inflammatory disease, and its incidence is steadily increasing. IBD is closely related to the intestinal microbiota, and probiotics are known to be a potential therapeutic agent for IBD. In our study, we evaluated the protective effect of <i>Lactobacillus sakei</i> CVL-001, isolated from <i>Baechu</i> kimchi, on dextran sulfated sodium (DSS)-induced colitis in mice. The oral administration of <i>L. sakei</i> CVL-001 according to the experimental schedule alleviated weight loss and disease activity in the mice with colitis. Furthermore, the length and histopathology of the colon improved. The expression of tumor necrosis factor (<i>TNF</i>)<i>-α</i> and interleukin <i>(IL)-1β</i> genes decreased in the colons of mice that were administered <i>L. sakei</i> CVL-001, whereas that of <i>IL-10</i> increased. The expressions of genes coding for E-cadherin, claudin3, occludin, and mucin were also restored. In co-housed conditions, <i>L. sakei</i> CVL-001 administration did not improve disease activity, colon length, and histopathology. Microbiota analysis revealed that <i>L. sakei</i> CVL-001 administration increased the abundance of microbiota and altered <i>Firmicutes</i>/<i>Bacteroidetes</i> ratio, and decreased <i>Proteobacteria</i>. In conclusion, <i>L. sakei</i> CVL-001 administration protects mice from DSS-induced colitis by regulating immune response and intestinal integrity via gut microbiota modulation.https://www.mdpi.com/2076-2607/11/5/1359inflammatory bowel disease<i>Lactobacillus sakei</i> CVL-001anti-inflammationepithelial barriermicrobiota |
spellingShingle | Dong-Yeon Kim Tae-Sung Lee Do-Hyeon Jung Eun-Jung Song Ah-Ra Jang Ji-Yeon Park Jae-Hun Ahn In-Su Seo Seung-Ju Song Yeong-Jun Kim Yun-Ji Lee Yeon-Ji Lee Jong-Hwan Park Oral Administration of <i>Lactobacillus sakei</i> CVL-001 Improves Recovery from Dextran Sulfate Sodium-Induced Colitis in Mice by Microbiota Modulation Microorganisms inflammatory bowel disease <i>Lactobacillus sakei</i> CVL-001 anti-inflammation epithelial barrier microbiota |
title | Oral Administration of <i>Lactobacillus sakei</i> CVL-001 Improves Recovery from Dextran Sulfate Sodium-Induced Colitis in Mice by Microbiota Modulation |
title_full | Oral Administration of <i>Lactobacillus sakei</i> CVL-001 Improves Recovery from Dextran Sulfate Sodium-Induced Colitis in Mice by Microbiota Modulation |
title_fullStr | Oral Administration of <i>Lactobacillus sakei</i> CVL-001 Improves Recovery from Dextran Sulfate Sodium-Induced Colitis in Mice by Microbiota Modulation |
title_full_unstemmed | Oral Administration of <i>Lactobacillus sakei</i> CVL-001 Improves Recovery from Dextran Sulfate Sodium-Induced Colitis in Mice by Microbiota Modulation |
title_short | Oral Administration of <i>Lactobacillus sakei</i> CVL-001 Improves Recovery from Dextran Sulfate Sodium-Induced Colitis in Mice by Microbiota Modulation |
title_sort | oral administration of i lactobacillus sakei i cvl 001 improves recovery from dextran sulfate sodium induced colitis in mice by microbiota modulation |
topic | inflammatory bowel disease <i>Lactobacillus sakei</i> CVL-001 anti-inflammation epithelial barrier microbiota |
url | https://www.mdpi.com/2076-2607/11/5/1359 |
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