Oral Administration of <i>Lactobacillus sakei</i> CVL-001 Improves Recovery from Dextran Sulfate Sodium-Induced Colitis in Mice by Microbiota Modulation

Inflammatory bowel disease (IBD) is an intestinal chronic inflammatory disease, and its incidence is steadily increasing. IBD is closely related to the intestinal microbiota, and probiotics are known to be a potential therapeutic agent for IBD. In our study, we evaluated the protective effect of <...

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Main Authors: Dong-Yeon Kim, Tae-Sung Lee, Do-Hyeon Jung, Eun-Jung Song, Ah-Ra Jang, Ji-Yeon Park, Jae-Hun Ahn, In-Su Seo, Seung-Ju Song, Yeong-Jun Kim, Yun-Ji Lee, Yeon-Ji Lee, Jong-Hwan Park
Format: Article
Language:English
Published: MDPI AG 2023-05-01
Series:Microorganisms
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Online Access:https://www.mdpi.com/2076-2607/11/5/1359
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author Dong-Yeon Kim
Tae-Sung Lee
Do-Hyeon Jung
Eun-Jung Song
Ah-Ra Jang
Ji-Yeon Park
Jae-Hun Ahn
In-Su Seo
Seung-Ju Song
Yeong-Jun Kim
Yun-Ji Lee
Yeon-Ji Lee
Jong-Hwan Park
author_facet Dong-Yeon Kim
Tae-Sung Lee
Do-Hyeon Jung
Eun-Jung Song
Ah-Ra Jang
Ji-Yeon Park
Jae-Hun Ahn
In-Su Seo
Seung-Ju Song
Yeong-Jun Kim
Yun-Ji Lee
Yeon-Ji Lee
Jong-Hwan Park
author_sort Dong-Yeon Kim
collection DOAJ
description Inflammatory bowel disease (IBD) is an intestinal chronic inflammatory disease, and its incidence is steadily increasing. IBD is closely related to the intestinal microbiota, and probiotics are known to be a potential therapeutic agent for IBD. In our study, we evaluated the protective effect of <i>Lactobacillus sakei</i> CVL-001, isolated from <i>Baechu</i> kimchi, on dextran sulfated sodium (DSS)-induced colitis in mice. The oral administration of <i>L. sakei</i> CVL-001 according to the experimental schedule alleviated weight loss and disease activity in the mice with colitis. Furthermore, the length and histopathology of the colon improved. The expression of tumor necrosis factor (<i>TNF</i>)<i>-α</i> and interleukin <i>(IL)-1β</i> genes decreased in the colons of mice that were administered <i>L. sakei</i> CVL-001, whereas that of <i>IL-10</i> increased. The expressions of genes coding for E-cadherin, claudin3, occludin, and mucin were also restored. In co-housed conditions, <i>L. sakei</i> CVL-001 administration did not improve disease activity, colon length, and histopathology. Microbiota analysis revealed that <i>L. sakei</i> CVL-001 administration increased the abundance of microbiota and altered <i>Firmicutes</i>/<i>Bacteroidetes</i> ratio, and decreased <i>Proteobacteria</i>. In conclusion, <i>L. sakei</i> CVL-001 administration protects mice from DSS-induced colitis by regulating immune response and intestinal integrity via gut microbiota modulation.
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spelling doaj.art-ebaab14a69e74f82b2b39b3f379d15022023-11-18T02:35:37ZengMDPI AGMicroorganisms2076-26072023-05-01115135910.3390/microorganisms11051359Oral Administration of <i>Lactobacillus sakei</i> CVL-001 Improves Recovery from Dextran Sulfate Sodium-Induced Colitis in Mice by Microbiota ModulationDong-Yeon Kim0Tae-Sung Lee1Do-Hyeon Jung2Eun-Jung Song3Ah-Ra Jang4Ji-Yeon Park5Jae-Hun Ahn6In-Su Seo7Seung-Ju Song8Yeong-Jun Kim9Yun-Ji Lee10Yeon-Ji Lee11Jong-Hwan Park12Laboratory Animal Medicine, Animal Medical Institute, College of Veterinary Medicine, Chonnam National University, 77 Yongbong-ro, Buk-gu, Gwangju 61186, Republic of KoreaLaboratory Animal Medicine, Animal Medical Institute, College of Veterinary Medicine, Chonnam National University, 77 Yongbong-ro, Buk-gu, Gwangju 61186, Republic of KoreaLaboratory Animal Medicine, Animal Medical Institute, College of Veterinary Medicine, Chonnam National University, 77 Yongbong-ro, Buk-gu, Gwangju 61186, Republic of KoreaNodcure, Inc., 77 Yongbong-ro, Buk-gu, Gwangju 61186, Republic of KoreaNodcure, Inc., 77 Yongbong-ro, Buk-gu, Gwangju 61186, Republic of KoreaLaboratory Animal Medicine, Animal Medical Institute, College of Veterinary Medicine, Chonnam National University, 77 Yongbong-ro, Buk-gu, Gwangju 61186, Republic of KoreaLaboratory Animal Medicine, Animal Medical Institute, College of Veterinary Medicine, Chonnam National University, 77 Yongbong-ro, Buk-gu, Gwangju 61186, Republic of KoreaLaboratory Animal Medicine, Animal Medical Institute, College of Veterinary Medicine, Chonnam National University, 77 Yongbong-ro, Buk-gu, Gwangju 61186, Republic of KoreaLaboratory Animal Medicine, Animal Medical Institute, College of Veterinary Medicine, Chonnam National University, 77 Yongbong-ro, Buk-gu, Gwangju 61186, Republic of KoreaLaboratory Animal Medicine, Animal Medical Institute, College of Veterinary Medicine, Chonnam National University, 77 Yongbong-ro, Buk-gu, Gwangju 61186, Republic of KoreaLaboratory Animal Medicine, Animal Medical Institute, College of Veterinary Medicine, Chonnam National University, 77 Yongbong-ro, Buk-gu, Gwangju 61186, Republic of KoreaLaboratory Animal Medicine, Animal Medical Institute, College of Veterinary Medicine, Chonnam National University, 77 Yongbong-ro, Buk-gu, Gwangju 61186, Republic of KoreaLaboratory Animal Medicine, Animal Medical Institute, College of Veterinary Medicine, Chonnam National University, 77 Yongbong-ro, Buk-gu, Gwangju 61186, Republic of KoreaInflammatory bowel disease (IBD) is an intestinal chronic inflammatory disease, and its incidence is steadily increasing. IBD is closely related to the intestinal microbiota, and probiotics are known to be a potential therapeutic agent for IBD. In our study, we evaluated the protective effect of <i>Lactobacillus sakei</i> CVL-001, isolated from <i>Baechu</i> kimchi, on dextran sulfated sodium (DSS)-induced colitis in mice. The oral administration of <i>L. sakei</i> CVL-001 according to the experimental schedule alleviated weight loss and disease activity in the mice with colitis. Furthermore, the length and histopathology of the colon improved. The expression of tumor necrosis factor (<i>TNF</i>)<i>-α</i> and interleukin <i>(IL)-1β</i> genes decreased in the colons of mice that were administered <i>L. sakei</i> CVL-001, whereas that of <i>IL-10</i> increased. The expressions of genes coding for E-cadherin, claudin3, occludin, and mucin were also restored. In co-housed conditions, <i>L. sakei</i> CVL-001 administration did not improve disease activity, colon length, and histopathology. Microbiota analysis revealed that <i>L. sakei</i> CVL-001 administration increased the abundance of microbiota and altered <i>Firmicutes</i>/<i>Bacteroidetes</i> ratio, and decreased <i>Proteobacteria</i>. In conclusion, <i>L. sakei</i> CVL-001 administration protects mice from DSS-induced colitis by regulating immune response and intestinal integrity via gut microbiota modulation.https://www.mdpi.com/2076-2607/11/5/1359inflammatory bowel disease<i>Lactobacillus sakei</i> CVL-001anti-inflammationepithelial barriermicrobiota
spellingShingle Dong-Yeon Kim
Tae-Sung Lee
Do-Hyeon Jung
Eun-Jung Song
Ah-Ra Jang
Ji-Yeon Park
Jae-Hun Ahn
In-Su Seo
Seung-Ju Song
Yeong-Jun Kim
Yun-Ji Lee
Yeon-Ji Lee
Jong-Hwan Park
Oral Administration of <i>Lactobacillus sakei</i> CVL-001 Improves Recovery from Dextran Sulfate Sodium-Induced Colitis in Mice by Microbiota Modulation
Microorganisms
inflammatory bowel disease
<i>Lactobacillus sakei</i> CVL-001
anti-inflammation
epithelial barrier
microbiota
title Oral Administration of <i>Lactobacillus sakei</i> CVL-001 Improves Recovery from Dextran Sulfate Sodium-Induced Colitis in Mice by Microbiota Modulation
title_full Oral Administration of <i>Lactobacillus sakei</i> CVL-001 Improves Recovery from Dextran Sulfate Sodium-Induced Colitis in Mice by Microbiota Modulation
title_fullStr Oral Administration of <i>Lactobacillus sakei</i> CVL-001 Improves Recovery from Dextran Sulfate Sodium-Induced Colitis in Mice by Microbiota Modulation
title_full_unstemmed Oral Administration of <i>Lactobacillus sakei</i> CVL-001 Improves Recovery from Dextran Sulfate Sodium-Induced Colitis in Mice by Microbiota Modulation
title_short Oral Administration of <i>Lactobacillus sakei</i> CVL-001 Improves Recovery from Dextran Sulfate Sodium-Induced Colitis in Mice by Microbiota Modulation
title_sort oral administration of i lactobacillus sakei i cvl 001 improves recovery from dextran sulfate sodium induced colitis in mice by microbiota modulation
topic inflammatory bowel disease
<i>Lactobacillus sakei</i> CVL-001
anti-inflammation
epithelial barrier
microbiota
url https://www.mdpi.com/2076-2607/11/5/1359
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