Effect of Dietary Modulation of Selenium Form and Level on Performance, Tissue Retention, Quality of Frozen Stored Meat and Gene Expression of Antioxidant Status in Ross Broiler Chickens
This study compares between different selenium forms (sodium selenite; SeS, selenomethionine; Met-Se or nano-Se) and levels on growth performance, Se retention, antioxidative potential of fresh and frozen meat, and genes related to oxidative stress in Ross broilers. Birds (n = 450) were randomly div...
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| Format: | Article |
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MDPI AG
2019-06-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/9/6/342 |
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| author | Doaa Ibrahim Asmaa T.Y. Kishawy Safaa I. Khater Ahmed Hamed Arisha Haiam A. Mohammed Ahmed Shaban Abdelaziz Ghada I. Abd El-Rahman Mohamed Tharwat Elabbasy |
| author_facet | Doaa Ibrahim Asmaa T.Y. Kishawy Safaa I. Khater Ahmed Hamed Arisha Haiam A. Mohammed Ahmed Shaban Abdelaziz Ghada I. Abd El-Rahman Mohamed Tharwat Elabbasy |
| author_sort | Doaa Ibrahim |
| collection | DOAJ |
| description | This study compares between different selenium forms (sodium selenite; SeS, selenomethionine; Met-Se or nano-Se) and levels on growth performance, Se retention, antioxidative potential of fresh and frozen meat, and genes related to oxidative stress in Ross broilers. Birds (n = 450) were randomly divided into nine experimental groups with five replicates in each and were fed diets supplemented with 0.3, 0.45, and 0.6 mg Se/kg as (SeS, Met-Se), or nano-Se. For overall growth performance, dietary inclusion of Met-Se or nano-Se significantly increased (<i>p</i> < 0.05) body weight gain and improved the feed conversion ratio of Ross broiler chicks at the level of 0.45 and 0.6 mg/kg when compared with the group fed the same level of SeS. Se sources and levels significantly affected (<i>p</i> < 0.05) its concentrations in breast muscle, liver, and serum. Moreover, Se retention in muscle was higher (<i>p</i> < 0.05) after feeding of broiler chicks on a diet supplemented with Met-Se or nano-Se compared to the SeS group, especially at 0.6 mg/kg. Additionally, higher dietary levels from Met-Se or nano-Se significantly reduced oxidative changes in breast and thigh meat in the fresh state and after a four-week storage period and increased muscular pH after 24 h of slaughter. Also, broiler’s meat in the Met-Se and nano-Se groups showed cooking loss and lower drip compared to the SeS group (<i>p</i> < 0.05). In the liver, the mRNA expression levels of glutathione peroxidase, superoxide dismutase, and catalase were elevated by increasing dietary Se levels from Met-Se and nano-Se groups up to 0.6 mg/kg when compared with SeS. Therefore, dietary supplementation with 0.6 mg/kg Met-Se and nano-Se improved growth performance and were more efficiently retained than with SeS. Both sources of selenium (Met-Se and nano-Se) downregulated the oxidation processes of meat during the first four weeks of frozen storage, especially in thigh meat, compared with an inorganic source. Finally, dietary supplementation of Met-Se and nano-Se produced acceptable Se levels in chicken meat offered for consumers. |
| first_indexed | 2024-12-15T00:17:11Z |
| format | Article |
| id | doaj.art-ebab646d78c9481a9df591b650b96a8d |
| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-12-15T00:17:11Z |
| publishDate | 2019-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-ebab646d78c9481a9df591b650b96a8d2022-12-21T22:42:26ZengMDPI AGAnimals2076-26152019-06-019634210.3390/ani9060342ani9060342Effect of Dietary Modulation of Selenium Form and Level on Performance, Tissue Retention, Quality of Frozen Stored Meat and Gene Expression of Antioxidant Status in Ross Broiler ChickensDoaa Ibrahim0Asmaa T.Y. Kishawy1Safaa I. Khater2Ahmed Hamed Arisha3Haiam A. Mohammed4Ahmed Shaban Abdelaziz5Ghada I. Abd El-Rahman6Mohamed Tharwat Elabbasy7Department of Nutrition and Clinical Nutrition, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44519, EgyptDepartment of Nutrition and Clinical Nutrition, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44519, EgyptDepartment of Biochemistry, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44519, EgyptDepartment of Physiology, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44519, EgyptDepartment of Physiology, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44519, EgyptDepartment of Pharmacology, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44519, EgyptDepartment of Clinical Pathology, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44519, EgyptCollege of Public Health and Molecular Diagnostics and Personalized Therapeutics Center (CMDPT) Hail University, Hail 2440, Saudi ArabiaThis study compares between different selenium forms (sodium selenite; SeS, selenomethionine; Met-Se or nano-Se) and levels on growth performance, Se retention, antioxidative potential of fresh and frozen meat, and genes related to oxidative stress in Ross broilers. Birds (n = 450) were randomly divided into nine experimental groups with five replicates in each and were fed diets supplemented with 0.3, 0.45, and 0.6 mg Se/kg as (SeS, Met-Se), or nano-Se. For overall growth performance, dietary inclusion of Met-Se or nano-Se significantly increased (<i>p</i> < 0.05) body weight gain and improved the feed conversion ratio of Ross broiler chicks at the level of 0.45 and 0.6 mg/kg when compared with the group fed the same level of SeS. Se sources and levels significantly affected (<i>p</i> < 0.05) its concentrations in breast muscle, liver, and serum. Moreover, Se retention in muscle was higher (<i>p</i> < 0.05) after feeding of broiler chicks on a diet supplemented with Met-Se or nano-Se compared to the SeS group, especially at 0.6 mg/kg. Additionally, higher dietary levels from Met-Se or nano-Se significantly reduced oxidative changes in breast and thigh meat in the fresh state and after a four-week storage period and increased muscular pH after 24 h of slaughter. Also, broiler’s meat in the Met-Se and nano-Se groups showed cooking loss and lower drip compared to the SeS group (<i>p</i> < 0.05). In the liver, the mRNA expression levels of glutathione peroxidase, superoxide dismutase, and catalase were elevated by increasing dietary Se levels from Met-Se and nano-Se groups up to 0.6 mg/kg when compared with SeS. Therefore, dietary supplementation with 0.6 mg/kg Met-Se and nano-Se improved growth performance and were more efficiently retained than with SeS. Both sources of selenium (Met-Se and nano-Se) downregulated the oxidation processes of meat during the first four weeks of frozen storage, especially in thigh meat, compared with an inorganic source. Finally, dietary supplementation of Met-Se and nano-Se produced acceptable Se levels in chicken meat offered for consumers.https://www.mdpi.com/2076-2615/9/6/342broilersselenium sources-levelsselenium retentionantioxidant capacityfrozen meat |
| spellingShingle | Doaa Ibrahim Asmaa T.Y. Kishawy Safaa I. Khater Ahmed Hamed Arisha Haiam A. Mohammed Ahmed Shaban Abdelaziz Ghada I. Abd El-Rahman Mohamed Tharwat Elabbasy Effect of Dietary Modulation of Selenium Form and Level on Performance, Tissue Retention, Quality of Frozen Stored Meat and Gene Expression of Antioxidant Status in Ross Broiler Chickens Animals broilers selenium sources-levels selenium retention antioxidant capacity frozen meat |
| title | Effect of Dietary Modulation of Selenium Form and Level on Performance, Tissue Retention, Quality of Frozen Stored Meat and Gene Expression of Antioxidant Status in Ross Broiler Chickens |
| title_full | Effect of Dietary Modulation of Selenium Form and Level on Performance, Tissue Retention, Quality of Frozen Stored Meat and Gene Expression of Antioxidant Status in Ross Broiler Chickens |
| title_fullStr | Effect of Dietary Modulation of Selenium Form and Level on Performance, Tissue Retention, Quality of Frozen Stored Meat and Gene Expression of Antioxidant Status in Ross Broiler Chickens |
| title_full_unstemmed | Effect of Dietary Modulation of Selenium Form and Level on Performance, Tissue Retention, Quality of Frozen Stored Meat and Gene Expression of Antioxidant Status in Ross Broiler Chickens |
| title_short | Effect of Dietary Modulation of Selenium Form and Level on Performance, Tissue Retention, Quality of Frozen Stored Meat and Gene Expression of Antioxidant Status in Ross Broiler Chickens |
| title_sort | effect of dietary modulation of selenium form and level on performance tissue retention quality of frozen stored meat and gene expression of antioxidant status in ross broiler chickens |
| topic | broilers selenium sources-levels selenium retention antioxidant capacity frozen meat |
| url | https://www.mdpi.com/2076-2615/9/6/342 |
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