Transcriptome Analysis of the Nematode Caenorhabditis elegans in Acidic Stress Environments
Ocean acidification and acid rain, caused by modern industries’ fossil fuel burning, lead to a decrease in the living environmental pH, which results in a series of negative effects on many organisms. However, the underlying mechanisms of animals’ response to acidic pH stress are largely unknown. In...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-09-01
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| Series: | Frontiers in Physiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fphys.2020.01107/full |
| _version_ | 1819013079598366720 |
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| author | Yanyi Cong Yanyi Cong Yanyi Cong Yanyi Cong Hanwen Yang Hanwen Yang Hanwen Yang Pengchi Zhang Pengchi Zhang Pengchi Zhang Pengchi Zhang Yusu Xie Yusu Xie Yusu Xie Xuwen Cao Xuwen Cao Xuwen Cao Xuwen Cao Liusuo Zhang Liusuo Zhang Liusuo Zhang |
| author_facet | Yanyi Cong Yanyi Cong Yanyi Cong Yanyi Cong Hanwen Yang Hanwen Yang Hanwen Yang Pengchi Zhang Pengchi Zhang Pengchi Zhang Pengchi Zhang Yusu Xie Yusu Xie Yusu Xie Xuwen Cao Xuwen Cao Xuwen Cao Xuwen Cao Liusuo Zhang Liusuo Zhang Liusuo Zhang |
| author_sort | Yanyi Cong |
| collection | DOAJ |
| description | Ocean acidification and acid rain, caused by modern industries’ fossil fuel burning, lead to a decrease in the living environmental pH, which results in a series of negative effects on many organisms. However, the underlying mechanisms of animals’ response to acidic pH stress are largely unknown. In this study, we used the nematode Caenorhabditis elegans as an animal model to explore the regulatory mechanisms of organisms’ response to pH decline. Two major stress-responsive pathways were found through transcriptome analysis in acidic stress environments. First, when the pH dropped from 6.33 to 4.33, the worms responded to the pH stress by upregulation of the col, nas, and dpy genes, which are required for cuticle synthesis and structure integrity. Second, when the pH continued to decrease from 4.33, the metabolism of xenobiotics by cytochrome P450 pathway genes (cyp, gst, ugt, and ABC transporters) played a major role in protecting the nematodes from the toxic substances probably produced by the more acidic environment. At the same time, the slowing down of cuticle synthesis might be due to its insufficient protective ability. Moreover, the systematic regulation pattern we found in nematodes might also be applied to other invertebrate and vertebrate animals to survive in the changing pH environments. Thus, our data might lay the foundation to identify the master gene(s) responding and adapting to acidic pH stress in further studies, and might also provide new solutions to improve assessment and monitoring of ecological restoration outcomes, or generate novel genotypes via genome editing for restoring in challenging environments especially in the context of acidic stress through global climate change. |
| first_indexed | 2024-12-21T01:54:15Z |
| format | Article |
| id | doaj.art-ebad520cd5a440bdb50e434a154d6de6 |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-12-21T01:54:15Z |
| publishDate | 2020-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-ebad520cd5a440bdb50e434a154d6de62022-12-21T19:19:49ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2020-09-011110.3389/fphys.2020.01107531135Transcriptome Analysis of the Nematode Caenorhabditis elegans in Acidic Stress EnvironmentsYanyi Cong0Yanyi Cong1Yanyi Cong2Yanyi Cong3Hanwen Yang4Hanwen Yang5Hanwen Yang6Pengchi Zhang7Pengchi Zhang8Pengchi Zhang9Pengchi Zhang10Yusu Xie11Yusu Xie12Yusu Xie13Xuwen Cao14Xuwen Cao15Xuwen Cao16Xuwen Cao17Liusuo Zhang18Liusuo Zhang19Liusuo Zhang20CAS Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, ChinaLaboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, ChinaCenter for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaCAS Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, ChinaLaboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, ChinaCenter for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, ChinaCAS Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, ChinaLaboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, ChinaCenter for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaCAS Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, ChinaLaboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, ChinaCenter for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, ChinaCAS Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, ChinaLaboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, ChinaCenter for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaCAS Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, ChinaLaboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, ChinaCenter for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, ChinaOcean acidification and acid rain, caused by modern industries’ fossil fuel burning, lead to a decrease in the living environmental pH, which results in a series of negative effects on many organisms. However, the underlying mechanisms of animals’ response to acidic pH stress are largely unknown. In this study, we used the nematode Caenorhabditis elegans as an animal model to explore the regulatory mechanisms of organisms’ response to pH decline. Two major stress-responsive pathways were found through transcriptome analysis in acidic stress environments. First, when the pH dropped from 6.33 to 4.33, the worms responded to the pH stress by upregulation of the col, nas, and dpy genes, which are required for cuticle synthesis and structure integrity. Second, when the pH continued to decrease from 4.33, the metabolism of xenobiotics by cytochrome P450 pathway genes (cyp, gst, ugt, and ABC transporters) played a major role in protecting the nematodes from the toxic substances probably produced by the more acidic environment. At the same time, the slowing down of cuticle synthesis might be due to its insufficient protective ability. Moreover, the systematic regulation pattern we found in nematodes might also be applied to other invertebrate and vertebrate animals to survive in the changing pH environments. Thus, our data might lay the foundation to identify the master gene(s) responding and adapting to acidic pH stress in further studies, and might also provide new solutions to improve assessment and monitoring of ecological restoration outcomes, or generate novel genotypes via genome editing for restoring in challenging environments especially in the context of acidic stress through global climate change.https://www.frontiersin.org/article/10.3389/fphys.2020.01107/fullpH stressacidic environmentcuticle collagensxenobiotic detoxificationP450Caenorhabditis elegans |
| spellingShingle | Yanyi Cong Yanyi Cong Yanyi Cong Yanyi Cong Hanwen Yang Hanwen Yang Hanwen Yang Pengchi Zhang Pengchi Zhang Pengchi Zhang Pengchi Zhang Yusu Xie Yusu Xie Yusu Xie Xuwen Cao Xuwen Cao Xuwen Cao Xuwen Cao Liusuo Zhang Liusuo Zhang Liusuo Zhang Transcriptome Analysis of the Nematode Caenorhabditis elegans in Acidic Stress Environments Frontiers in Physiology pH stress acidic environment cuticle collagens xenobiotic detoxification P450 Caenorhabditis elegans |
| title | Transcriptome Analysis of the Nematode Caenorhabditis elegans in Acidic Stress Environments |
| title_full | Transcriptome Analysis of the Nematode Caenorhabditis elegans in Acidic Stress Environments |
| title_fullStr | Transcriptome Analysis of the Nematode Caenorhabditis elegans in Acidic Stress Environments |
| title_full_unstemmed | Transcriptome Analysis of the Nematode Caenorhabditis elegans in Acidic Stress Environments |
| title_short | Transcriptome Analysis of the Nematode Caenorhabditis elegans in Acidic Stress Environments |
| title_sort | transcriptome analysis of the nematode caenorhabditis elegans in acidic stress environments |
| topic | pH stress acidic environment cuticle collagens xenobiotic detoxification P450 Caenorhabditis elegans |
| url | https://www.frontiersin.org/article/10.3389/fphys.2020.01107/full |
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