Development and validation of a sensitive enzyme-linked immunosorbent assay for clonidine hydrochloride in pig urine and pork samples
Clonidine hydrochloride (CLO) is a new substitute for a traditionally used adrenergic agonist. The illegal use of CLO in the livestock industry possess potential harm to human health. Hence, it is an urgent need for the rapid detection of CLO residues. Here, we prepared a highly sensitive and specif...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2021-01-01
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| Series: | Food and Agricultural Immunology |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/09540105.2021.2001439 |
| _version_ | 1828884849713217536 |
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| author | Yao Wang Jinbo Cao Yaning Sun Yunrui Xing Xinghao Pang Xiujin Chen Peng Fei Zhaozhou Li Qiaofen Cheng Huaibin Kang Xiaofei Hu |
| author_facet | Yao Wang Jinbo Cao Yaning Sun Yunrui Xing Xinghao Pang Xiujin Chen Peng Fei Zhaozhou Li Qiaofen Cheng Huaibin Kang Xiaofei Hu |
| author_sort | Yao Wang |
| collection | DOAJ |
| description | Clonidine hydrochloride (CLO) is a new substitute for a traditionally used adrenergic agonist. The illegal use of CLO in the livestock industry possess potential harm to human health. Hence, it is an urgent need for the rapid detection of CLO residues. Here, we prepared a highly sensitive and specific monoclonal antibody (mAb) and it used to develop an indirect competitive ELISA (ic-ELISA) for the rapid screening of CLO residues. The limit of detection and limit of quantification values of ic-ELISA were as follows: 0.033 and 0.054 ng/mL for pig urine and 0.061 and 0.096 ng/mL for pork, respectively. Recovery experiment indicated that the ic-ELISA posed outstanding accuracy and precision. Furthermore, the results of ic-ELISA were strongly correlated to the results of HPLC. Thus, the ic-ELISA provided a sensitive and rapid on-site detection of CLO residues in pig urine and pork samples. |
| first_indexed | 2024-12-13T11:15:50Z |
| format | Article |
| id | doaj.art-ebb26b3ca4f94299ad990e6049804640 |
| institution | Directory Open Access Journal |
| issn | 0954-0105 1465-3443 |
| language | English |
| last_indexed | 2024-12-13T11:15:50Z |
| publishDate | 2021-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Food and Agricultural Immunology |
| spelling | doaj.art-ebb26b3ca4f94299ad990e60498046402022-12-21T23:48:37ZengTaylor & Francis GroupFood and Agricultural Immunology0954-01051465-34432021-01-0132185186210.1080/09540105.2021.20014392001439Development and validation of a sensitive enzyme-linked immunosorbent assay for clonidine hydrochloride in pig urine and pork samplesYao Wang0Jinbo Cao1Yaning Sun2Yunrui Xing3Xinghao Pang4Xiujin Chen5Peng Fei6Zhaozhou Li7Qiaofen Cheng8Huaibin Kang9Xiaofei Hu10Henan University of Science and TechnologyHenan Academy of Agricultural SciencesHenan Academy of Agricultural SciencesHenan Academy of Agricultural SciencesHenan University of Science and TechnologyHenan University of Science and TechnologyHenan University of Science and TechnologyHenan University of Science and TechnologyUniversity of ReadingHenan University of Science and TechnologyHenan Academy of Agricultural SciencesClonidine hydrochloride (CLO) is a new substitute for a traditionally used adrenergic agonist. The illegal use of CLO in the livestock industry possess potential harm to human health. Hence, it is an urgent need for the rapid detection of CLO residues. Here, we prepared a highly sensitive and specific monoclonal antibody (mAb) and it used to develop an indirect competitive ELISA (ic-ELISA) for the rapid screening of CLO residues. The limit of detection and limit of quantification values of ic-ELISA were as follows: 0.033 and 0.054 ng/mL for pig urine and 0.061 and 0.096 ng/mL for pork, respectively. Recovery experiment indicated that the ic-ELISA posed outstanding accuracy and precision. Furthermore, the results of ic-ELISA were strongly correlated to the results of HPLC. Thus, the ic-ELISA provided a sensitive and rapid on-site detection of CLO residues in pig urine and pork samples.http://dx.doi.org/10.1080/09540105.2021.2001439clonidine hydrochloridemonoclonal antibodyic-elisa |
| spellingShingle | Yao Wang Jinbo Cao Yaning Sun Yunrui Xing Xinghao Pang Xiujin Chen Peng Fei Zhaozhou Li Qiaofen Cheng Huaibin Kang Xiaofei Hu Development and validation of a sensitive enzyme-linked immunosorbent assay for clonidine hydrochloride in pig urine and pork samples Food and Agricultural Immunology clonidine hydrochloride monoclonal antibody ic-elisa |
| title | Development and validation of a sensitive enzyme-linked immunosorbent assay for clonidine hydrochloride in pig urine and pork samples |
| title_full | Development and validation of a sensitive enzyme-linked immunosorbent assay for clonidine hydrochloride in pig urine and pork samples |
| title_fullStr | Development and validation of a sensitive enzyme-linked immunosorbent assay for clonidine hydrochloride in pig urine and pork samples |
| title_full_unstemmed | Development and validation of a sensitive enzyme-linked immunosorbent assay for clonidine hydrochloride in pig urine and pork samples |
| title_short | Development and validation of a sensitive enzyme-linked immunosorbent assay for clonidine hydrochloride in pig urine and pork samples |
| title_sort | development and validation of a sensitive enzyme linked immunosorbent assay for clonidine hydrochloride in pig urine and pork samples |
| topic | clonidine hydrochloride monoclonal antibody ic-elisa |
| url | http://dx.doi.org/10.1080/09540105.2021.2001439 |
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