496 Urinary Exosomal MicroRNA as Early Markers of Diabetic Kidney Disease in African American Adults
OBJECTIVES/GOALS: This study aimed to characterize urinary exosomal miRNA content in African American adults with diabetic kidney disease. METHODS/STUDY POPULATION: Male and female participants between the ages of 18 and 65 were recruited from the Diabetes Treatment Center and the Nephrology Clinic...
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| Format: | Article |
| Language: | English |
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Cambridge University Press
2024-04-01
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| Series: | Journal of Clinical and Translational Science |
| Online Access: | https://www.cambridge.org/core/product/identifier/S2059866124004217/type/journal_article |
| _version_ | 1827296129319960576 |
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| author | Maurice B. Fluitt Neal Mohit Mykaiya Sumling Baiyee-Ndang Agbor-Baiyee Kanwal K. Gambhir Gail Nunlee-Bland Constance Mere Maurice B. Fluitt |
| author_facet | Maurice B. Fluitt Neal Mohit Mykaiya Sumling Baiyee-Ndang Agbor-Baiyee Kanwal K. Gambhir Gail Nunlee-Bland Constance Mere Maurice B. Fluitt |
| author_sort | Maurice B. Fluitt |
| collection | DOAJ |
| description | OBJECTIVES/GOALS: This study aimed to characterize urinary exosomal miRNA content in African American adults with diabetic kidney disease. METHODS/STUDY POPULATION: Male and female participants between the ages of 18 and 65 were recruited from the Diabetes Treatment Center and the Nephrology Clinic at the Howard University Hospital. Exosomes were isolated from cleared urine of healthy controls (n=3), type 2 diabetics (n=3), and participants with chronic kidney disease (n=3). The purity and size of isolated microparticles was evaluated using NanoSight technology (30nm to 120nm size range) and western blot analysis for exosome-specific markers (TSG101 and CD81) RESULTS/ANTICIPATED RESULTS: Expression of 5 selected microRNAs, miR-4534, miR-320c, miR-451, miR-362-3p and miR-877-3p were evaluated by qRT-PCR. miR-4534 and miR-451 was increased between healthy controls and the type diabetic group. MiR-320c was increased in the CKD group, in comparison to healthy controls. Conversely, there was no difference in miR-877-5p between the three groups. DISCUSSION/SIGNIFICANCE: These findings will provide insight into the use of circulating miRNAs as early markers of DKD, ultimately creating more effective treatments and preventive measures. |
| first_indexed | 2024-04-24T14:32:36Z |
| format | Article |
| id | doaj.art-ebb6557d86294880a543f23cd72a4e47 |
| institution | Directory Open Access Journal |
| issn | 2059-8661 |
| language | English |
| last_indexed | 2024-04-24T14:32:36Z |
| publishDate | 2024-04-01 |
| publisher | Cambridge University Press |
| record_format | Article |
| series | Journal of Clinical and Translational Science |
| spelling | doaj.art-ebb6557d86294880a543f23cd72a4e472024-04-03T02:00:11ZengCambridge University PressJournal of Clinical and Translational Science2059-86612024-04-01814614710.1017/cts.2024.421496 Urinary Exosomal MicroRNA as Early Markers of Diabetic Kidney Disease in African American AdultsMaurice B. Fluitt0Neal Mohit1Mykaiya Sumling2Baiyee-Ndang Agbor-Baiyee3Kanwal K. Gambhir4Gail Nunlee-Bland5Constance Mere6Maurice B. Fluitt7Howard UniversityEndocrinology and Metabolism, Department of Medicine, Howard University College of MedicineDepartment of Biology, Howard UniversityEndocrinology and Metabolism, Department of Medicine, Howard University College of MedicineEndocrinology and Metabolism, Department of Medicine, Howard University College of MedicineDiabetes Treatment Center, Howard University HospitalDivision of Nephrology, Department of Medicine, Howard University College of MedicineEndocrinology and Metabolism, Department of Medicine, Howard University College of Medicine Laboratory of Epigenetic and Metabolic Research, Department of Medicine, Howard University College of MedicineOBJECTIVES/GOALS: This study aimed to characterize urinary exosomal miRNA content in African American adults with diabetic kidney disease. METHODS/STUDY POPULATION: Male and female participants between the ages of 18 and 65 were recruited from the Diabetes Treatment Center and the Nephrology Clinic at the Howard University Hospital. Exosomes were isolated from cleared urine of healthy controls (n=3), type 2 diabetics (n=3), and participants with chronic kidney disease (n=3). The purity and size of isolated microparticles was evaluated using NanoSight technology (30nm to 120nm size range) and western blot analysis for exosome-specific markers (TSG101 and CD81) RESULTS/ANTICIPATED RESULTS: Expression of 5 selected microRNAs, miR-4534, miR-320c, miR-451, miR-362-3p and miR-877-3p were evaluated by qRT-PCR. miR-4534 and miR-451 was increased between healthy controls and the type diabetic group. MiR-320c was increased in the CKD group, in comparison to healthy controls. Conversely, there was no difference in miR-877-5p between the three groups. DISCUSSION/SIGNIFICANCE: These findings will provide insight into the use of circulating miRNAs as early markers of DKD, ultimately creating more effective treatments and preventive measures.https://www.cambridge.org/core/product/identifier/S2059866124004217/type/journal_article |
| spellingShingle | Maurice B. Fluitt Neal Mohit Mykaiya Sumling Baiyee-Ndang Agbor-Baiyee Kanwal K. Gambhir Gail Nunlee-Bland Constance Mere Maurice B. Fluitt 496 Urinary Exosomal MicroRNA as Early Markers of Diabetic Kidney Disease in African American Adults Journal of Clinical and Translational Science |
| title | 496 Urinary Exosomal MicroRNA as Early Markers of Diabetic Kidney Disease in African American Adults |
| title_full | 496 Urinary Exosomal MicroRNA as Early Markers of Diabetic Kidney Disease in African American Adults |
| title_fullStr | 496 Urinary Exosomal MicroRNA as Early Markers of Diabetic Kidney Disease in African American Adults |
| title_full_unstemmed | 496 Urinary Exosomal MicroRNA as Early Markers of Diabetic Kidney Disease in African American Adults |
| title_short | 496 Urinary Exosomal MicroRNA as Early Markers of Diabetic Kidney Disease in African American Adults |
| title_sort | 496 urinary exosomal microrna as early markers of diabetic kidney disease in african american adults |
| url | https://www.cambridge.org/core/product/identifier/S2059866124004217/type/journal_article |
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