Cellular Fragments in the Perivitelline Space Are Not a Predictor of Expanded Blastocyst Quality
The presence of cellular fragments in the perivitelline space is a commonly used parameter to determine quality before transfer of in vitro produced (IVP) embryos. However, this parameter is difficult to assess after blastocyst expansion. In this study, we used mechanical hatching to confirm the pre...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-01-01
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| Series: | Frontiers in Cell and Developmental Biology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2020.616801/full |
| _version_ | 1819051597655703552 |
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| author | Bo Yu Helena T. A. van Tol Tom A. E. Stout Bernard A. J. Roelen |
| author_facet | Bo Yu Helena T. A. van Tol Tom A. E. Stout Bernard A. J. Roelen |
| author_sort | Bo Yu |
| collection | DOAJ |
| description | The presence of cellular fragments in the perivitelline space is a commonly used parameter to determine quality before transfer of in vitro produced (IVP) embryos. However, this parameter is difficult to assess after blastocyst expansion. In this study, we used mechanical hatching to confirm the presence of cellular fragments in the perivitelline space of bovine IVP blastocysts. We further looked for associations between possible apoptosis within extruded cells/ cellular fragments and the quality of bovine blastocysts using quantitative RT-PCR and immunofluorescence. Surprisingly, more than 42% of expanded blastocysts had cellular fragments in the perivitelline space; however, more than 37% of extruded cells were TUNEL negative. We observed no significant difference in embryo quality between expanded blastocysts with and without cellular fragments in the perivitelline space. Overall, our data suggest that embryos extrude abnormal cells to maintain their developmental potential. The presence of fragmented cells is not an indicator of embryo quality. |
| first_indexed | 2024-12-21T12:06:28Z |
| format | Article |
| id | doaj.art-ebb6f2b5f6294862aa7b8060e0338f3e |
| institution | Directory Open Access Journal |
| issn | 2296-634X |
| language | English |
| last_indexed | 2024-12-21T12:06:28Z |
| publishDate | 2021-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj.art-ebb6f2b5f6294862aa7b8060e0338f3e2022-12-21T19:04:41ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2021-01-01810.3389/fcell.2020.616801616801Cellular Fragments in the Perivitelline Space Are Not a Predictor of Expanded Blastocyst QualityBo Yu0Helena T. A. van Tol1Tom A. E. Stout2Bernard A. J. Roelen3Farm Animal Health, Department of Population Health Sciences, Faculty of Veterinary Medicine, Utrecht University, Utrecht, NetherlandsFarm Animal Health, Department of Population Health Sciences, Faculty of Veterinary Medicine, Utrecht University, Utrecht, NetherlandsEquine Sciences, Department Clinical Sciences, Faculty of Veterinary Medicine, Utrecht University, Utrecht, NetherlandsEmbryology, Anatomy and Physiology, Department Clinical Sciences, Faculty of Veterinary Medicine, Utrecht University, Utrecht, NetherlandsThe presence of cellular fragments in the perivitelline space is a commonly used parameter to determine quality before transfer of in vitro produced (IVP) embryos. However, this parameter is difficult to assess after blastocyst expansion. In this study, we used mechanical hatching to confirm the presence of cellular fragments in the perivitelline space of bovine IVP blastocysts. We further looked for associations between possible apoptosis within extruded cells/ cellular fragments and the quality of bovine blastocysts using quantitative RT-PCR and immunofluorescence. Surprisingly, more than 42% of expanded blastocysts had cellular fragments in the perivitelline space; however, more than 37% of extruded cells were TUNEL negative. We observed no significant difference in embryo quality between expanded blastocysts with and without cellular fragments in the perivitelline space. Overall, our data suggest that embryos extrude abnormal cells to maintain their developmental potential. The presence of fragmented cells is not an indicator of embryo quality.https://www.frontiersin.org/articles/10.3389/fcell.2020.616801/fullbovinezona pellucidacellular fragmentsapoptosisembryo qualityblastocyst |
| spellingShingle | Bo Yu Helena T. A. van Tol Tom A. E. Stout Bernard A. J. Roelen Cellular Fragments in the Perivitelline Space Are Not a Predictor of Expanded Blastocyst Quality Frontiers in Cell and Developmental Biology bovine zona pellucida cellular fragments apoptosis embryo quality blastocyst |
| title | Cellular Fragments in the Perivitelline Space Are Not a Predictor of Expanded Blastocyst Quality |
| title_full | Cellular Fragments in the Perivitelline Space Are Not a Predictor of Expanded Blastocyst Quality |
| title_fullStr | Cellular Fragments in the Perivitelline Space Are Not a Predictor of Expanded Blastocyst Quality |
| title_full_unstemmed | Cellular Fragments in the Perivitelline Space Are Not a Predictor of Expanded Blastocyst Quality |
| title_short | Cellular Fragments in the Perivitelline Space Are Not a Predictor of Expanded Blastocyst Quality |
| title_sort | cellular fragments in the perivitelline space are not a predictor of expanded blastocyst quality |
| topic | bovine zona pellucida cellular fragments apoptosis embryo quality blastocyst |
| url | https://www.frontiersin.org/articles/10.3389/fcell.2020.616801/full |
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