Anatomical and single-cell transcriptional profiling of the murine habenular complex
The lateral habenula (LHb) is an epithalamic brain structure critical for processing and adapting to negative action outcomes. However, despite the importance of LHb to behavior and the clear anatomical and molecular diversity of LHb neurons, the neuron types of the habenula remain unknown. Here, we...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2020-02-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/51271 |
| _version_ | 1818028281016877056 |
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| author | Michael L Wallace Kee Wui Huang Daniel Hochbaum Minsuk Hyun Gianna Radeljic Bernardo L Sabatini |
| author_facet | Michael L Wallace Kee Wui Huang Daniel Hochbaum Minsuk Hyun Gianna Radeljic Bernardo L Sabatini |
| author_sort | Michael L Wallace |
| collection | DOAJ |
| description | The lateral habenula (LHb) is an epithalamic brain structure critical for processing and adapting to negative action outcomes. However, despite the importance of LHb to behavior and the clear anatomical and molecular diversity of LHb neurons, the neuron types of the habenula remain unknown. Here, we use high-throughput single-cell transcriptional profiling, monosynaptic retrograde tracing, and multiplexed FISH to characterize the cells of the mouse habenula. We find five subtypes of neurons in the medial habenula (MHb) that are organized into anatomical subregions. In the LHb, we describe four neuronal subtypes and show that they differentially target dopaminergic and GABAergic cells in the ventral tegmental area (VTA). These data provide a valuable resource for future study of habenular function and dysfunction and demonstrate neuronal subtype specificity in the LHb-VTA circuit. |
| first_indexed | 2024-12-10T05:01:18Z |
| format | Article |
| id | doaj.art-ebb868683f164c43a9b519659127a7ec |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-12-10T05:01:18Z |
| publishDate | 2020-02-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-ebb868683f164c43a9b519659127a7ec2022-12-22T02:01:24ZengeLife Sciences Publications LtdeLife2050-084X2020-02-01910.7554/eLife.51271Anatomical and single-cell transcriptional profiling of the murine habenular complexMichael L Wallace0https://orcid.org/0000-0002-7270-8521Kee Wui Huang1https://orcid.org/0000-0003-2265-4550Daniel Hochbaum2Minsuk Hyun3Gianna Radeljic4Bernardo L Sabatini5https://orcid.org/0000-0003-0095-9177Department of Neurobiology, Howard Hughes Medical Institute, Harvard Medical School, Boston, United StatesDepartment of Neurobiology, Howard Hughes Medical Institute, Harvard Medical School, Boston, United StatesDepartment of Neurobiology, Howard Hughes Medical Institute, Harvard Medical School, Boston, United StatesDepartment of Neurobiology, Howard Hughes Medical Institute, Harvard Medical School, Boston, United StatesDepartment of Neurobiology, Howard Hughes Medical Institute, Harvard Medical School, Boston, United StatesDepartment of Neurobiology, Howard Hughes Medical Institute, Harvard Medical School, Boston, United StatesThe lateral habenula (LHb) is an epithalamic brain structure critical for processing and adapting to negative action outcomes. However, despite the importance of LHb to behavior and the clear anatomical and molecular diversity of LHb neurons, the neuron types of the habenula remain unknown. Here, we use high-throughput single-cell transcriptional profiling, monosynaptic retrograde tracing, and multiplexed FISH to characterize the cells of the mouse habenula. We find five subtypes of neurons in the medial habenula (MHb) that are organized into anatomical subregions. In the LHb, we describe four neuronal subtypes and show that they differentially target dopaminergic and GABAergic cells in the ventral tegmental area (VTA). These data provide a valuable resource for future study of habenular function and dysfunction and demonstrate neuronal subtype specificity in the LHb-VTA circuit.https://elifesciences.org/articles/51271habenulaVTAdopamineserotonin |
| spellingShingle | Michael L Wallace Kee Wui Huang Daniel Hochbaum Minsuk Hyun Gianna Radeljic Bernardo L Sabatini Anatomical and single-cell transcriptional profiling of the murine habenular complex eLife habenula VTA dopamine serotonin |
| title | Anatomical and single-cell transcriptional profiling of the murine habenular complex |
| title_full | Anatomical and single-cell transcriptional profiling of the murine habenular complex |
| title_fullStr | Anatomical and single-cell transcriptional profiling of the murine habenular complex |
| title_full_unstemmed | Anatomical and single-cell transcriptional profiling of the murine habenular complex |
| title_short | Anatomical and single-cell transcriptional profiling of the murine habenular complex |
| title_sort | anatomical and single cell transcriptional profiling of the murine habenular complex |
| topic | habenula VTA dopamine serotonin |
| url | https://elifesciences.org/articles/51271 |
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