Development of resistant tomato population with bacterial canker resistance genes from interspecific hybrids by the support of embryo rescue
Bacterial canker is one of the most important diseases causing economic yield loss in tomato production areas in the world. The best way to control for this disease is to use resistant varieties. However, there are few studies on variety breeding studies of this disease compared with other disease r...
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| Format: | Article |
| Language: | English |
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Bati Akdeniz Agricultural Research Institute
2016-06-01
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| Series: | Derim |
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| Online Access: | http://derim.com.tr/article/view/5000140545 |
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| author | Aylin KABAŞ Esin ARI Sinan ZENGİN Hülya İLBİ Yeşim AYSAN Asu OĞUZ |
| author_facet | Aylin KABAŞ Esin ARI Sinan ZENGİN Hülya İLBİ Yeşim AYSAN Asu OĞUZ |
| author_sort | Aylin KABAŞ |
| collection | DOAJ |
| description | Bacterial canker is one of the most important diseases causing economic yield loss in tomato production areas in the world. The best way to control for this disease is to use resistant varieties. However, there are few studies on variety breeding studies of this disease compared with other disease resistant breeding studies. In this study we aimed to improve inbred lines carrying bacterial canker resistance genes to use in the breeding of resistant varieties. Susceptible inbred line AK1 (S. esculentum) and resistant LA2157 (S. peruvianum) were crossed. Embryo rescue and ovule culture techniques were applied in 30 fruits to get F1 hybrids. Rescued embryos and immature ovules were cultured in petri dishes containing solidified MS medium without hormone. 30 healty embryos were excised and cultured from 30 fruits 27-61 day old (1 embryo fruit-1) in embryo rescue method. The two surviving plants from acclimatization were transferred to the greenhouse to get their BC1 progenies. Resistance tests were performed according to the stem inoculation method in the BC1 and BC2 progenies. The mixture of 14 aggressive Turkish Cmm strains were used to confirm the resistance. The plants were valued by 0-4 scale. Plants with 0 and 1 scale values were used to obtain next progenies. A total of 80 BC3 resistant progenies were transferred to our variety breeding programme. |
| first_indexed | 2024-04-10T13:31:46Z |
| format | Article |
| id | doaj.art-ebbb0e7f401a42cb92945cb8a07bb78e |
| institution | Directory Open Access Journal |
| issn | 1300-3496 |
| language | English |
| last_indexed | 2024-04-10T13:31:46Z |
| publishDate | 2016-06-01 |
| publisher | Bati Akdeniz Agricultural Research Institute |
| record_format | Article |
| series | Derim |
| spelling | doaj.art-ebbb0e7f401a42cb92945cb8a07bb78e2023-02-15T16:11:35ZengBati Akdeniz Agricultural Research InstituteDerim1300-34962016-06-01331274610.16882/derim.2016.07605Development of resistant tomato population with bacterial canker resistance genes from interspecific hybrids by the support of embryo rescueAylin KABAŞ0Esin ARI1Sinan ZENGİN 2 Hülya İLBİ 3Yeşim AYSAN 4Asu OĞUZ5Batı Akdeniz Agricultural Research Institute, Antalya, TurkeyAkdeniz University Agricultural Faculty Agricultural Biotechnology Department, Antalya, TurkeyAntalya Tarim, Antalya, TurkeyEge University Agricultural Faculty Horticulture Department, İzmir, TurkeyCukurova University Agricultural Faculty Plant Protection Department, Adana, TurkeyAntalya Agricultural Quarantine Directorate, Antalya, TurkeyBacterial canker is one of the most important diseases causing economic yield loss in tomato production areas in the world. The best way to control for this disease is to use resistant varieties. However, there are few studies on variety breeding studies of this disease compared with other disease resistant breeding studies. In this study we aimed to improve inbred lines carrying bacterial canker resistance genes to use in the breeding of resistant varieties. Susceptible inbred line AK1 (S. esculentum) and resistant LA2157 (S. peruvianum) were crossed. Embryo rescue and ovule culture techniques were applied in 30 fruits to get F1 hybrids. Rescued embryos and immature ovules were cultured in petri dishes containing solidified MS medium without hormone. 30 healty embryos were excised and cultured from 30 fruits 27-61 day old (1 embryo fruit-1) in embryo rescue method. The two surviving plants from acclimatization were transferred to the greenhouse to get their BC1 progenies. Resistance tests were performed according to the stem inoculation method in the BC1 and BC2 progenies. The mixture of 14 aggressive Turkish Cmm strains were used to confirm the resistance. The plants were valued by 0-4 scale. Plants with 0 and 1 scale values were used to obtain next progenies. A total of 80 BC3 resistant progenies were transferred to our variety breeding programme.http://derim.com.tr/article/view/5000140545TomatoClavibacterEmbryo rescueBreeding |
| spellingShingle | Aylin KABAŞ Esin ARI Sinan ZENGİN Hülya İLBİ Yeşim AYSAN Asu OĞUZ Development of resistant tomato population with bacterial canker resistance genes from interspecific hybrids by the support of embryo rescue Derim Tomato Clavibacter Embryo rescue Breeding |
| title | Development of resistant tomato population with bacterial canker resistance genes from interspecific hybrids by the support of embryo rescue |
| title_full | Development of resistant tomato population with bacterial canker resistance genes from interspecific hybrids by the support of embryo rescue |
| title_fullStr | Development of resistant tomato population with bacterial canker resistance genes from interspecific hybrids by the support of embryo rescue |
| title_full_unstemmed | Development of resistant tomato population with bacterial canker resistance genes from interspecific hybrids by the support of embryo rescue |
| title_short | Development of resistant tomato population with bacterial canker resistance genes from interspecific hybrids by the support of embryo rescue |
| title_sort | development of resistant tomato population with bacterial canker resistance genes from interspecific hybrids by the support of embryo rescue |
| topic | Tomato Clavibacter Embryo rescue Breeding |
| url | http://derim.com.tr/article/view/5000140545 |
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