Assessment of the Nutraceutical Effects of Oleuropein and the Cytotoxic Effects of Adriamycin, When Administered Alone and in Combination, in MG-63 Human Osteosarcoma Cells
Oleuropein (OLEU) is the most distinguished phenolic compound found in olive fruit and the leaves of <i>Olea europaea</i> L., with several pharmacological properties, including anti-cancer actions. Adriamycin (ADR) is an anthracycline widely used as a chemotherapeutic agent, although it...
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MDPI AG
2021-01-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/13/2/354 |
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| author | Katerina Gioti Anastasia Papachristodoulou Dimitra Benaki Nektarios Aligiannis Alexios-Leandros Skaltsounis Emmanuel Mikros Roxane Tenta |
| author_facet | Katerina Gioti Anastasia Papachristodoulou Dimitra Benaki Nektarios Aligiannis Alexios-Leandros Skaltsounis Emmanuel Mikros Roxane Tenta |
| author_sort | Katerina Gioti |
| collection | DOAJ |
| description | Oleuropein (OLEU) is the most distinguished phenolic compound found in olive fruit and the leaves of <i>Olea europaea</i> L., with several pharmacological properties, including anti-cancer actions. Adriamycin (ADR) is an anthracycline widely used as a chemotherapeutic agent, although it presents significant side effects. The aim of the present study was to investigate the effect of oleuropein alone (20 μg/mL) and in co-treatment with ADR (50 nM), in MG-63 human osteosarcoma cells. Therefore, cellular and molecular techniques, such as MTT assay, flow cytometry, real-time Polymerase Chain Reaction (PCR), western blot and Elisa method, as well as Nuclear Magnetic Resonance (NMR) spectroscopy, were applied to unveil changes in the signal transduction pathways involved in osteosarcoma cells survival. The observed alterations in gene, protein and metabolite levels denote that OLEU not only inhibits MG-63 cells proliferation and potentiates ADR’s cytotoxicity, but also exerts its action, at least in part, through the induction of autophagy. |
| first_indexed | 2024-03-09T03:44:54Z |
| format | Article |
| id | doaj.art-ebbbdd5376c549428cd1db77e6db3e32 |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-09T03:44:54Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-ebbbdd5376c549428cd1db77e6db3e322023-12-03T14:35:58ZengMDPI AGNutrients2072-66432021-01-0113235410.3390/nu13020354Assessment of the Nutraceutical Effects of Oleuropein and the Cytotoxic Effects of Adriamycin, When Administered Alone and in Combination, in MG-63 Human Osteosarcoma CellsKaterina Gioti0Anastasia Papachristodoulou1Dimitra Benaki2Nektarios Aligiannis3Alexios-Leandros Skaltsounis4Emmanuel Mikros5Roxane Tenta6Department of Nutrition and Dietetics, School of Health Science and Education, Harokopio University, Kallithea, 17671 Athens, GreeceDepartment of Nutrition and Dietetics, School of Health Science and Education, Harokopio University, Kallithea, 17671 Athens, GreeceDepartment of Pharmacy, Section of Pharmaceutical Chemistry, National and Kapodostrian University of Athens, Panepistimiopolis, Zografou, 15771 Athens, GreeceDepartment of Pharmacy, Section of Pharmacognosy and Natural Products Chemistry, National and Kapodistrian University of Athens, Panepistimiopolis, Zografou, 15771 Athens, GreeceDepartment of Pharmacy, Section of Pharmacognosy and Natural Products Chemistry, National and Kapodistrian University of Athens, Panepistimiopolis, Zografou, 15771 Athens, GreeceDepartment of Pharmacy, Section of Pharmaceutical Chemistry, National and Kapodostrian University of Athens, Panepistimiopolis, Zografou, 15771 Athens, GreeceDepartment of Nutrition and Dietetics, School of Health Science and Education, Harokopio University, Kallithea, 17671 Athens, GreeceOleuropein (OLEU) is the most distinguished phenolic compound found in olive fruit and the leaves of <i>Olea europaea</i> L., with several pharmacological properties, including anti-cancer actions. Adriamycin (ADR) is an anthracycline widely used as a chemotherapeutic agent, although it presents significant side effects. The aim of the present study was to investigate the effect of oleuropein alone (20 μg/mL) and in co-treatment with ADR (50 nM), in MG-63 human osteosarcoma cells. Therefore, cellular and molecular techniques, such as MTT assay, flow cytometry, real-time Polymerase Chain Reaction (PCR), western blot and Elisa method, as well as Nuclear Magnetic Resonance (NMR) spectroscopy, were applied to unveil changes in the signal transduction pathways involved in osteosarcoma cells survival. The observed alterations in gene, protein and metabolite levels denote that OLEU not only inhibits MG-63 cells proliferation and potentiates ADR’s cytotoxicity, but also exerts its action, at least in part, through the induction of autophagy.https://www.mdpi.com/2072-6643/13/2/354oleuropeinadriamycinosteosarcoma |
| spellingShingle | Katerina Gioti Anastasia Papachristodoulou Dimitra Benaki Nektarios Aligiannis Alexios-Leandros Skaltsounis Emmanuel Mikros Roxane Tenta Assessment of the Nutraceutical Effects of Oleuropein and the Cytotoxic Effects of Adriamycin, When Administered Alone and in Combination, in MG-63 Human Osteosarcoma Cells Nutrients oleuropein adriamycin osteosarcoma |
| title | Assessment of the Nutraceutical Effects of Oleuropein and the Cytotoxic Effects of Adriamycin, When Administered Alone and in Combination, in MG-63 Human Osteosarcoma Cells |
| title_full | Assessment of the Nutraceutical Effects of Oleuropein and the Cytotoxic Effects of Adriamycin, When Administered Alone and in Combination, in MG-63 Human Osteosarcoma Cells |
| title_fullStr | Assessment of the Nutraceutical Effects of Oleuropein and the Cytotoxic Effects of Adriamycin, When Administered Alone and in Combination, in MG-63 Human Osteosarcoma Cells |
| title_full_unstemmed | Assessment of the Nutraceutical Effects of Oleuropein and the Cytotoxic Effects of Adriamycin, When Administered Alone and in Combination, in MG-63 Human Osteosarcoma Cells |
| title_short | Assessment of the Nutraceutical Effects of Oleuropein and the Cytotoxic Effects of Adriamycin, When Administered Alone and in Combination, in MG-63 Human Osteosarcoma Cells |
| title_sort | assessment of the nutraceutical effects of oleuropein and the cytotoxic effects of adriamycin when administered alone and in combination in mg 63 human osteosarcoma cells |
| topic | oleuropein adriamycin osteosarcoma |
| url | https://www.mdpi.com/2072-6643/13/2/354 |
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