Antifungal and anti-biofilm activities of patchouli alcohol against Candida albicans
The opportunistic fungal pathogen Candida albicans could cause severe clinical outcomes which could be exacerbated by the scarcity of antifungals. The capacity of C. albicans to form biofilms on medical devices that are hard to eradicate, further deepen the need to develop antifungal agents. In this...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-03-01
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| Series: | International Journal of Medical Microbiology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1438422123000243 |
| _version_ | 1797256451171811328 |
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| author | Qiulei Zhang Jingxiao Zhang Yu Zhang Yujie Sui Yang Du Longfei Yang Yongjie Yin |
| author_facet | Qiulei Zhang Jingxiao Zhang Yu Zhang Yujie Sui Yang Du Longfei Yang Yongjie Yin |
| author_sort | Qiulei Zhang |
| collection | DOAJ |
| description | The opportunistic fungal pathogen Candida albicans could cause severe clinical outcomes which could be exacerbated by the scarcity of antifungals. The capacity of C. albicans to form biofilms on medical devices that are hard to eradicate, further deepen the need to develop antifungal agents. In this study, we, for the first time, showed that patchouli alcohol (PA) can inhibit the growth of multiple C. albicans strains, as well as four other Candida species, with MICs of 64 μg/mL and MFCs from 64 to 128 μg/mL. The biofilm formation and development, adhesion, yeast-to-hyphal transition and extracellular polysaccharide of C. albicans can be inhibited by PA in a concentration-dependent manner. Confocal microscopy analyses of cells treated with PA showed that PA can increase the membrane permeability and intracellular reactive oxygen species (ROS) production. In C. elegans, PA did not influence the survival below 64 μg/mL. In this study PA demonstrated antifungal and antibiofilm activity against C. albicans and our results showed the potential of developing PA to fight Candida infections. |
| first_indexed | 2024-03-08T21:32:42Z |
| format | Article |
| id | doaj.art-ebbda53ece054d83ae0b7437c17fd5fd |
| institution | Directory Open Access Journal |
| issn | 1438-4221 |
| language | English |
| last_indexed | 2024-04-24T22:21:57Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | International Journal of Medical Microbiology |
| spelling | doaj.art-ebbda53ece054d83ae0b7437c17fd5fd2024-03-20T06:08:44ZengElsevierInternational Journal of Medical Microbiology1438-42212024-03-01314151596Antifungal and anti-biofilm activities of patchouli alcohol against Candida albicansQiulei Zhang0Jingxiao Zhang1Yu Zhang2Yujie Sui3Yang Du4Longfei Yang5Yongjie Yin6Department of Emergency and Critical Care Medicine, The Second Hospital of Jilin University, Changchun 130041, ChinaDepartment of Emergency and Critical Care Medicine, The Second Hospital of Jilin University, Changchun 130041, ChinaDepartment of Clinical Laboratory, The Second Hospital of Jilin University, 218# Ziqiang Street, Changchun 130041, ChinaJilin Provincial Key Laboratory on Molecular and Chemical Genetics, The Second Hospital of Jilin University, 265# Ziqiang Street, Changchun 130041, ChinaEye Center, The Second Hospital of Jilin University, #4026 Yatai Street, Changchun 130024, ChinaJilin Provincial Key Laboratory on Molecular and Chemical Genetics, The Second Hospital of Jilin University, 265# Ziqiang Street, Changchun 130041, China; Corresponding authors.Department of Emergency and Critical Care Medicine, The Second Hospital of Jilin University, Changchun 130041, China; Corresponding authors.The opportunistic fungal pathogen Candida albicans could cause severe clinical outcomes which could be exacerbated by the scarcity of antifungals. The capacity of C. albicans to form biofilms on medical devices that are hard to eradicate, further deepen the need to develop antifungal agents. In this study, we, for the first time, showed that patchouli alcohol (PA) can inhibit the growth of multiple C. albicans strains, as well as four other Candida species, with MICs of 64 μg/mL and MFCs from 64 to 128 μg/mL. The biofilm formation and development, adhesion, yeast-to-hyphal transition and extracellular polysaccharide of C. albicans can be inhibited by PA in a concentration-dependent manner. Confocal microscopy analyses of cells treated with PA showed that PA can increase the membrane permeability and intracellular reactive oxygen species (ROS) production. In C. elegans, PA did not influence the survival below 64 μg/mL. In this study PA demonstrated antifungal and antibiofilm activity against C. albicans and our results showed the potential of developing PA to fight Candida infections.http://www.sciencedirect.com/science/article/pii/S1438422123000243Candida albicansPatchouli alcoholBiofilmVirulence factorsROS |
| spellingShingle | Qiulei Zhang Jingxiao Zhang Yu Zhang Yujie Sui Yang Du Longfei Yang Yongjie Yin Antifungal and anti-biofilm activities of patchouli alcohol against Candida albicans International Journal of Medical Microbiology Candida albicans Patchouli alcohol Biofilm Virulence factors ROS |
| title | Antifungal and anti-biofilm activities of patchouli alcohol against Candida albicans |
| title_full | Antifungal and anti-biofilm activities of patchouli alcohol against Candida albicans |
| title_fullStr | Antifungal and anti-biofilm activities of patchouli alcohol against Candida albicans |
| title_full_unstemmed | Antifungal and anti-biofilm activities of patchouli alcohol against Candida albicans |
| title_short | Antifungal and anti-biofilm activities of patchouli alcohol against Candida albicans |
| title_sort | antifungal and anti biofilm activities of patchouli alcohol against candida albicans |
| topic | Candida albicans Patchouli alcohol Biofilm Virulence factors ROS |
| url | http://www.sciencedirect.com/science/article/pii/S1438422123000243 |
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