An improved assay for platelet-activating factor using HPLC-tandem mass spectrometry

An improved assay for platelet-activating factor using HPLC-tandem mass spectrometry

We describe an improved assay for platelet-activating factor (PAF; 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine) using HPLC-tandem mass spectrometry (LC-MS/MS). The present method can readily detect as little as 1 pg (1.9 fmol) of PAF, a significant improvement over previously described LC-MS/MS m...

Full description

Bibliographic Details
Main Authors:	John S. Owen, Robert L. Wykle, Michael P. Samuel, Michael J. Thomas
Format:	Article
Language:	English
Published:	Elsevier 2005-02-01
Series:	Journal of Lipid Research
Subjects:	high-performance liquid chromatography tandem mass spectrometry trace analysis neutrophil
Online Access:	http://www.sciencedirect.com/science/article/pii/S0022227520340712

Similar Items

Determination of Chlorate and Perchlorate Amounts in Meat by High Performance Liquid Chromatography-Tandem Mass Spectrometry
by: Yanli YU, et al.
Published: (2023-06-01)

Simultaneous Determination of 9 Mycotoxins in Fruits by Ultra-performance Liquid Chromatography-Tandem Mass Spectrometry
by: Yang NI, et al.
Published: (2022-11-01)

Detection of Cashew Allergens in Foods by Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry
by: NING Yawei, YANG Zheng, MA Junmei, LIU Zhuo, CHEN Jia, LI Qiang
Published: (2023-02-01)

Bioanalysis of alpelisib using liquid chromatography–tandem mass spectrometry and application to pharmacokinetic study
by: Seop Lee, et al.
Published: (2022-08-01)

Development of a Reverse-Phase High-Performance Liquid Chromatography and Liquid Chromatography Tandem Mass Spectrometry Methods for Quality Control of Daegunjoong-Tang
by: Chang-Seob Seo, et al.
Published: (2021-04-01)

Tandem mass spectrometry : molecular characterization /
by: Coelho, Ana Varela, editor, et al.
Published: (2013)

Determination of nosiheptide residue in food animal tissues by high performance liquid chromatography-tandem mass spectrometry
by: NIE Chen, et al.
Published: (2022-07-01)

Quantitative tandem mass spectrometry in the clinical laboratory: Regulation and opportunity for validation of laboratory developed tests
by: Judith A. Stone, et al.
Published: (2023-04-01)

A novel fully-automated method to measure steroids in serum by liquid chromatography-tandem mass spectrometry
by: François Fraissinet, et al.
Published: (2023-01-01)

Hyperandrogenism by Liquid Chromatography Tandem Mass Spectrometry in PCOS: Focus on Testosterone and Androstenedione
by: Giorgia Grassi, et al.
Published: (2020-12-01)

Determination of icariside, hyperoside and psoralen in food by liquid chromatography-tandem mass spectrometry
by: Sufang Fan, et al.
Published: (2023-09-01)

Determination of Candesartan in Human Plasma using Liquid Chromatography - Tandem Mass Spectrometry
by: Vanja Forjan, et al.
Published: (2016-02-01)

Harmonized Collaborative Validation of Aflatoxins and Sterigmatocystin in White Rice and Sorghum by Liquid Chromatography Coupled to Tandem Mass Spectrometry
by: Hyun Ee Ok, et al.
Published: (2016-12-01)

Chemical characterization of the photodegradation products of midazolam complexes with randomly methylated-β-cyclodextrin by HPLC and LC-MS/MS
by: Agatonovic-Kustrin Snezana, et al.
Published: (2016-01-01)

Ultra-High-Performance Liquid Chromatography Tandem Mass Spectrometry Assay for Determination of Endogenous GHB and GHB-Glucuronide in Nails
by: Francesco Paolo Busardò, et al.
Published: (2018-10-01)

Determination of Pentachlorophenol in Milk Powder by Isotope Dilution Liquid Chromatography-Tandem Mass Spectrometry
by: QI Yan, LI Jinqing, LI Cong, XU Qingpeng
Published: (2023-08-01)

Determination of steroid esters in hair of slaughter animals by liquid chromatography with tandem mass spectrometry
by: Matraszek-Żuchowska Iwona, et al.
Published: (2019-10-01)

Determination of chloramphenicol residue in poultry eggs by QuEChERS combined with high performance liquid chromatography-tandem mass spectrometry
by: ZHAO Sa, et al.
Published: (2023-03-01)

Analysis of Lipids in Green Coffee by Ultra-Performance Liquid Chromatography–Time-of-Flight Tandem Mass Spectrometry
by: Yijun Liu, et al.
Published: (2022-08-01)

Determination of Hyaluronic Acid in Dairy Products by Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry
by: Guohui DU, et al.
Published: (2023-04-01)

Determination of 9 yellow colorants in fish tissues by ultra performance liquid chromatography-tandem mass spectrometry
by: PAN Ying, et al.
Published: (2022-01-01)

Determination of 11 Kinds of Organophosphorus Flame Retardants in Baby Rice Cereal by Ultra-high Performance Liquid Chromatography-tandem Mass Spectrometry
by: Xi WANG, et al.
Published: (2022-09-01)

Simultaneous analysis of allopurinol and oxypurinol using a validated liquid chromatography–tandem mass spectrometry method in human plasma
by: Dhiraj M. Rathod, et al.
Published: (2017-02-01)

Ultra-Performance Liquid Chromatography Tandem Mass Spectrometry Measurement of Caffeine in Caffeine-Laced Pants and in Urine and Skin of a Pants User
by: Manuela Pellegrini, et al.
Published: (2014-04-01)

Therapeutic drug monitoring of glycopeptide antimicrobials: An overview of liquid chromatography-tandem mass spectrometry methods
by: Alessia Cafaro, et al.
Published: (2024-01-01)

Quantification of All-Trans Retinoic Acid by Liquid Chromatography–Tandem Mass Spectrometry and Association with Lipid Profile in Patients with Type 2 Diabetes
by: Jakob Morgenstern, et al.
Published: (2021-01-01)

Determination of Lactoferrin in Camel Milk by Ultrahigh-Performance Liquid Chromatography-Tandem Mass Spectrometry Using an Isotope-Labeled Winged Peptide as Internal Standard
by: Xia Li, et al.
Published: (2019-11-01)

Tandem mass spectrometry of lipids : molecular analysis of complex lipids /
by: Murphy, Robert C author
Published: (2015)

Total serum pentosidine quantification using liquid chromatography-tandem mass spectrometry
by: Lindsie A. Blencowe, et al.
Published: (2024-03-01)

Determination of Methylene Blue and Its Metabolite Residues in Aquatic Products by High-Performance Liquid Chromatography–Tandem Mass Spectrometry
by: Xuan Zhang, et al.
Published: (2021-08-01)

Determination of six fluoroquinolones in aquatic products by QuEChERS-ultra performance liquid chromatography-tandem mass spectrometry
by: Rongxuan XUE, et al.
Published: (2020-09-01)

Simultaneous Determination of Coumarin and Its Derivatives in Tobacco Products by Liquid Chromatography-Tandem Mass Spectrometry
by: Zhiqin Ren, et al.
Published: (2016-11-01)

Determination of Residues of 5-Nitroimidazoles and Their Metabolites in Eggs by Isotope Dilution-Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry
by: QIU Xiaoyi, WANG Dongmei, LI Jiakuan, XU Qing, WANG Mengying, GUAN Zhuolong, LU Yuepeng, TU Fengqin
Published: (2023-11-01)

Determination of 8 Organic Pollutants in Drinking Waters by Ultra-high Performance Liquid Chromatography Tandem Mass Spectrometry with Direct Injection
by: YANG Li-xin, et al.
Published: (2014-03-01)

Determination of 8 Organic Pollutants in Drinking Waters by Ultra-high Performance Liquid Chromatography Tandem Mass Spectrometry with Direct Injection
by: YANG Li-xin, et al.
Published: (2014-03-01)

Simultaneous determination of 15 pesticide residues in Chinese cabbage and cucumber by liquid chromatography-tandem mass spectrometry utilizing online turbulent flow chromatography
by: Sufang Fan, et al.
Published: (2021-01-01)

Simultaneous Determination of 24 Sulfonamide Antibiotics in Fish Products by QuEChERS with Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry
by: Shuo LI, et al.
Published: (2022-05-01)

Simultaneous Determination of 85 Acid Synthetic Pigments in Sauce by Liquid Chromatography Tandem Mass Spectrometry
by: Zihao LIN, et al.
Published: (2022-03-01)

Analysis of Lipids in Pitaya Seed Oil by Ultra-Performance Liquid Chromatography–Time-of-Flight Tandem Mass Spectrometry
by: Yijun Liu, et al.
Published: (2022-09-01)

Chiral Determination of Naringenin by Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry and Application in Citrus Peel and Pulp
by: Zhoulin Yao, et al.
Published: (2022-06-01)