Efficient Transovarial Transmission of <i>Babesia</i> Spp. in <i>Rhipicephalus microplus</i> Ticks Fed on Water Buffalo (<i>Bubalus bubalis</i>)
Water buffaloes can be infected by tick-borne pathogens (TBPs) in endemic areas where cattle and buffalo coexist. Among TBPs affecting buffaloes is the Apicomplexan hemoparasites <i>Babesia bovis</i> and <i>B. bigemina</i>, transmitted by <i>Rhipicephalus microplus</...
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| Format: | Article |
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MDPI AG
2020-04-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/9/4/280 |
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| author | Dasiel Obregón Belkis Corona-González Adrian Alberto Díaz-Sánchez Yasmani Armas Eugenio Roque Márcia Cristina de Sena Oliveira Alejandro Cabezas-Cruz |
| author_facet | Dasiel Obregón Belkis Corona-González Adrian Alberto Díaz-Sánchez Yasmani Armas Eugenio Roque Márcia Cristina de Sena Oliveira Alejandro Cabezas-Cruz |
| author_sort | Dasiel Obregón |
| collection | DOAJ |
| description | Water buffaloes can be infected by tick-borne pathogens (TBPs) in endemic areas where cattle and buffalo coexist. Among TBPs affecting buffaloes is the Apicomplexan hemoparasites <i>Babesia bovis</i> and <i>B. bigemina</i>, transmitted by <i>Rhipicephalus microplus</i> ticks. However, little empirical evidence exists on whether buffalo can support TBPs’ infection and transmission. A cohort study was designed to measure the infestation levels of <i>R. microplus</i> in buffaloes as well as the ability of buffalo-fed ticks to transmit <i>B. bovis</i> and <i>B. bigemina</i> to their offspring. Tick infestation of different life stages was quantified in cattle and buffalo kept in field conditions in western Cuba. Engorged adult female ticks were allowed to lay eggs in controlled conditions of humidity and temperature, and reproductive parameters were measured and analyzed. Hosts and tick larvae were tested for the presence of <i>Babesia</i> spp. using species-specific qPCR assays. Tick infestation was not observed in adult buffaloes. However, buffalo and cattle calves were equally infested, although the larval survival rate was higher in cattle calves than in buffalo calves. All larval pools (31) obtained from the adult female ticks were positive for <i>B. bovis,</i> whereas only 68% (21/31) was positive for <i>B. bigemina</i>. Among the 10 larval pools negative for <i>B. bigemina</i>, three proceeded from adult females fed on <i>Babesia</i>-negative buffaloes. The other seven pools were from <i>Babesia</i>-positive animals, three from cattle and four from buffalo calves. <i>Babesia</i> infection levels in tick larvae, quantified by qPCR, were similar in female ticks fed on buffalo and bovine calves. We conclude that water buffalo can sustain tick vector populations and support <i>Babesia</i> infection in levels high enough as to be infective for ticks. Our results also validated the hypothesis that adult female ticks fed on buffalo can transmit the pathogens <i>B. bovis</i> and <i>B. bigemina</i> to their offspring. Nevertheless, further laboratory studies are needed to address the question of whether the transovarial transmission of <i>Babesia</i> occurs in the following settings: (1) When adult females are infected previous to the feeding on the buffalo or/and (2) when the adult females acquire the infection while feeding on the buffalo. |
| first_indexed | 2024-03-10T20:32:28Z |
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| id | doaj.art-ebc04d38968743518dbb7cbbf2649272 |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-10T20:32:28Z |
| publishDate | 2020-04-01 |
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| series | Pathogens |
| spelling | doaj.art-ebc04d38968743518dbb7cbbf26492722023-11-19T21:21:47ZengMDPI AGPathogens2076-08172020-04-019428010.3390/pathogens9040280Efficient Transovarial Transmission of <i>Babesia</i> Spp. in <i>Rhipicephalus microplus</i> Ticks Fed on Water Buffalo (<i>Bubalus bubalis</i>)Dasiel Obregón0Belkis Corona-González1Adrian Alberto Díaz-Sánchez2Yasmani Armas3Eugenio Roque4Márcia Cristina de Sena Oliveira5Alejandro Cabezas-Cruz6School of Environmental Sciences, University of Guelph, Guelph, ON N1G 2W1, CanadaCentro Nacional de Sanidad Agropecuaria (CENSA), San José de Las Lajas, Mayabeque 32700, CubaCentro Nacional de Sanidad Agropecuaria (CENSA), San José de Las Lajas, Mayabeque 32700, CubaFaculty of Veterinary Medicine, Universidad Agraria de La Habana, San José de Las Lajas, Mayabeque 32700, CubaFaculty of Veterinary Medicine, Universidad Agraria de La Habana, San José de Las Lajas, Mayabeque 32700, CubaEmbrapa Pecuária Sudeste, São Carlos, São Paulo 13560-970, BrazilUMR BIPAR, INRAE, ANSES, Ecole Nationale Vétérinaire d’Alfort, Université Paris-Est, 94700 Maisons-Alfort, FranceWater buffaloes can be infected by tick-borne pathogens (TBPs) in endemic areas where cattle and buffalo coexist. Among TBPs affecting buffaloes is the Apicomplexan hemoparasites <i>Babesia bovis</i> and <i>B. bigemina</i>, transmitted by <i>Rhipicephalus microplus</i> ticks. However, little empirical evidence exists on whether buffalo can support TBPs’ infection and transmission. A cohort study was designed to measure the infestation levels of <i>R. microplus</i> in buffaloes as well as the ability of buffalo-fed ticks to transmit <i>B. bovis</i> and <i>B. bigemina</i> to their offspring. Tick infestation of different life stages was quantified in cattle and buffalo kept in field conditions in western Cuba. Engorged adult female ticks were allowed to lay eggs in controlled conditions of humidity and temperature, and reproductive parameters were measured and analyzed. Hosts and tick larvae were tested for the presence of <i>Babesia</i> spp. using species-specific qPCR assays. Tick infestation was not observed in adult buffaloes. However, buffalo and cattle calves were equally infested, although the larval survival rate was higher in cattle calves than in buffalo calves. All larval pools (31) obtained from the adult female ticks were positive for <i>B. bovis,</i> whereas only 68% (21/31) was positive for <i>B. bigemina</i>. Among the 10 larval pools negative for <i>B. bigemina</i>, three proceeded from adult females fed on <i>Babesia</i>-negative buffaloes. The other seven pools were from <i>Babesia</i>-positive animals, three from cattle and four from buffalo calves. <i>Babesia</i> infection levels in tick larvae, quantified by qPCR, were similar in female ticks fed on buffalo and bovine calves. We conclude that water buffalo can sustain tick vector populations and support <i>Babesia</i> infection in levels high enough as to be infective for ticks. Our results also validated the hypothesis that adult female ticks fed on buffalo can transmit the pathogens <i>B. bovis</i> and <i>B. bigemina</i> to their offspring. Nevertheless, further laboratory studies are needed to address the question of whether the transovarial transmission of <i>Babesia</i> occurs in the following settings: (1) When adult females are infected previous to the feeding on the buffalo or/and (2) when the adult females acquire the infection while feeding on the buffalo.https://www.mdpi.com/2076-0817/9/4/280water buffalocattleticksreproductive efficiency<i>Babesia</i>qPCR |
| spellingShingle | Dasiel Obregón Belkis Corona-González Adrian Alberto Díaz-Sánchez Yasmani Armas Eugenio Roque Márcia Cristina de Sena Oliveira Alejandro Cabezas-Cruz Efficient Transovarial Transmission of <i>Babesia</i> Spp. in <i>Rhipicephalus microplus</i> Ticks Fed on Water Buffalo (<i>Bubalus bubalis</i>) Pathogens water buffalo cattle ticks reproductive efficiency <i>Babesia</i> qPCR |
| title | Efficient Transovarial Transmission of <i>Babesia</i> Spp. in <i>Rhipicephalus microplus</i> Ticks Fed on Water Buffalo (<i>Bubalus bubalis</i>) |
| title_full | Efficient Transovarial Transmission of <i>Babesia</i> Spp. in <i>Rhipicephalus microplus</i> Ticks Fed on Water Buffalo (<i>Bubalus bubalis</i>) |
| title_fullStr | Efficient Transovarial Transmission of <i>Babesia</i> Spp. in <i>Rhipicephalus microplus</i> Ticks Fed on Water Buffalo (<i>Bubalus bubalis</i>) |
| title_full_unstemmed | Efficient Transovarial Transmission of <i>Babesia</i> Spp. in <i>Rhipicephalus microplus</i> Ticks Fed on Water Buffalo (<i>Bubalus bubalis</i>) |
| title_short | Efficient Transovarial Transmission of <i>Babesia</i> Spp. in <i>Rhipicephalus microplus</i> Ticks Fed on Water Buffalo (<i>Bubalus bubalis</i>) |
| title_sort | efficient transovarial transmission of i babesia i spp in i rhipicephalus microplus i ticks fed on water buffalo i bubalus bubalis i |
| topic | water buffalo cattle ticks reproductive efficiency <i>Babesia</i> qPCR |
| url | https://www.mdpi.com/2076-0817/9/4/280 |
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