INHIBITION OF THE BACTERIAL GROWTH IN THE CULTURES OF STREPTOCOCCUS PYOGENES AND STREPTOCOCCUS AGALACTIAE IN PRESENCE OF COPPER AND ZINC IONS
Aim. The work was performed with the purpose to study antibacterial action of millimolar concentrations of copper and zinc ions, used as sulfates or chlorides in the cultures of S.pyogenes and S.agalactiae. Materials and methods. Suspensions of the S.pyogenes and S.agalactiae bacteria which containe...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | Russian |
| Published: |
Central Research Institute for Epidemiology
2017-06-01
|
| Series: | Журнал микробиологии, эпидемиологии и иммунобиологии |
| Subjects: | |
| Online Access: | https://microbiol.elpub.ru/jour/article/view/157 |
| _version_ | 1818081041263362048 |
|---|---|
| author | S. B. Cheknev E. I. Vostrova M. A. Sarycheva S. V. Kisil V. V. Anisimov A. V. Vostrov |
| author_facet | S. B. Cheknev E. I. Vostrova M. A. Sarycheva S. V. Kisil V. V. Anisimov A. V. Vostrov |
| author_sort | S. B. Cheknev |
| collection | DOAJ |
| description | Aim. The work was performed with the purpose to study antibacterial action of millimolar concentrations of copper and zinc ions, used as sulfates or chlorides in the cultures of S.pyogenes and S.agalactiae. Materials and methods. Suspensions of the S.pyogenes and S.agalactiae bacteria which contained 108 CFU/ml were sown by the lawns into the standard Petri dishes coated with the supplemented Nutrient Agar. 30 min later the salt solutions of copper or zinc were added by the 5 pi drops on the surfaces of the lawns with use of 36-channel stamp replicator. The salt solutions contained copper or zinc at the concentrations ranged between 5xl0-3 M to 5xl0_1 M of the metals. Then the dishes containing bacterial cultures were incubated for 24 hrs at 37°C followed by measuring diameter of the area of culture growth inhibition. The probes of material obtained from the centers of the stunting areas were passed into the centrifuge tubes with the supplemented Nutrient Broth, incubated for up to five days at 37°C and tested for the Broth clarity. Results. In presence of the metal concentrations ranged between 50 to 500 mM the inhibitory action of zinc sulfate towards S.pyogenes bacteria was 1.5 - 1.7 times higher (p |
| first_indexed | 2024-12-10T18:59:54Z |
| format | Article |
| id | doaj.art-ebc0a11c271a45a2bdb1af36d38ca934 |
| institution | Directory Open Access Journal |
| issn | 0372-9311 2686-7613 |
| language | Russian |
| last_indexed | 2024-12-10T18:59:54Z |
| publishDate | 2017-06-01 |
| publisher | Central Research Institute for Epidemiology |
| record_format | Article |
| series | Журнал микробиологии, эпидемиологии и иммунобиологии |
| spelling | doaj.art-ebc0a11c271a45a2bdb1af36d38ca9342022-12-22T01:37:02ZrusCentral Research Institute for EpidemiologyЖурнал микробиологии, эпидемиологии и иммунобиологии0372-93112686-76132017-06-0103263510.36233/0372-9311-2017-3-26-35157INHIBITION OF THE BACTERIAL GROWTH IN THE CULTURES OF STREPTOCOCCUS PYOGENES AND STREPTOCOCCUS AGALACTIAE IN PRESENCE OF COPPER AND ZINC IONSS. B. Cheknev0E. I. Vostrova1M. A. Sarycheva2S. V. Kisil3V. V. Anisimov4A. V. Vostrov5Gamaleya Federal Research Center of Epidemiology and MicrobiologyGamaleya Federal Research Center of Epidemiology and MicrobiologyGamaleya Federal Research Center of Epidemiology and MicrobiologyGamaleya Federal Research Center of Epidemiology and MicrobiologyGamaleya Federal Research Center of Epidemiology and MicrobiologyGamaleya Federal Research Center of Epidemiology and MicrobiologyAim. The work was performed with the purpose to study antibacterial action of millimolar concentrations of copper and zinc ions, used as sulfates or chlorides in the cultures of S.pyogenes and S.agalactiae. Materials and methods. Suspensions of the S.pyogenes and S.agalactiae bacteria which contained 108 CFU/ml were sown by the lawns into the standard Petri dishes coated with the supplemented Nutrient Agar. 30 min later the salt solutions of copper or zinc were added by the 5 pi drops on the surfaces of the lawns with use of 36-channel stamp replicator. The salt solutions contained copper or zinc at the concentrations ranged between 5xl0-3 M to 5xl0_1 M of the metals. Then the dishes containing bacterial cultures were incubated for 24 hrs at 37°C followed by measuring diameter of the area of culture growth inhibition. The probes of material obtained from the centers of the stunting areas were passed into the centrifuge tubes with the supplemented Nutrient Broth, incubated for up to five days at 37°C and tested for the Broth clarity. Results. In presence of the metal concentrations ranged between 50 to 500 mM the inhibitory action of zinc sulfate towards S.pyogenes bacteria was 1.5 - 1.7 times higher (phttps://microbiol.elpub.ru/jour/article/view/157s.pyogeness.agalactiaeantibacterial actioncopperzincs.pyogeness.agalactiae |
| spellingShingle | S. B. Cheknev E. I. Vostrova M. A. Sarycheva S. V. Kisil V. V. Anisimov A. V. Vostrov INHIBITION OF THE BACTERIAL GROWTH IN THE CULTURES OF STREPTOCOCCUS PYOGENES AND STREPTOCOCCUS AGALACTIAE IN PRESENCE OF COPPER AND ZINC IONS Журнал микробиологии, эпидемиологии и иммунобиологии s.pyogenes s.agalactiae antibacterial action copper zinc s.pyogenes s.agalactiae |
| title | INHIBITION OF THE BACTERIAL GROWTH IN THE CULTURES OF STREPTOCOCCUS PYOGENES AND STREPTOCOCCUS AGALACTIAE IN PRESENCE OF COPPER AND ZINC IONS |
| title_full | INHIBITION OF THE BACTERIAL GROWTH IN THE CULTURES OF STREPTOCOCCUS PYOGENES AND STREPTOCOCCUS AGALACTIAE IN PRESENCE OF COPPER AND ZINC IONS |
| title_fullStr | INHIBITION OF THE BACTERIAL GROWTH IN THE CULTURES OF STREPTOCOCCUS PYOGENES AND STREPTOCOCCUS AGALACTIAE IN PRESENCE OF COPPER AND ZINC IONS |
| title_full_unstemmed | INHIBITION OF THE BACTERIAL GROWTH IN THE CULTURES OF STREPTOCOCCUS PYOGENES AND STREPTOCOCCUS AGALACTIAE IN PRESENCE OF COPPER AND ZINC IONS |
| title_short | INHIBITION OF THE BACTERIAL GROWTH IN THE CULTURES OF STREPTOCOCCUS PYOGENES AND STREPTOCOCCUS AGALACTIAE IN PRESENCE OF COPPER AND ZINC IONS |
| title_sort | inhibition of the bacterial growth in the cultures of streptococcus pyogenes and streptococcus agalactiae in presence of copper and zinc ions |
| topic | s.pyogenes s.agalactiae antibacterial action copper zinc s.pyogenes s.agalactiae |
| url | https://microbiol.elpub.ru/jour/article/view/157 |
| work_keys_str_mv | AT sbcheknev inhibitionofthebacterialgrowthintheculturesofstreptococcuspyogenesandstreptococcusagalactiaeinpresenceofcopperandzincions AT eivostrova inhibitionofthebacterialgrowthintheculturesofstreptococcuspyogenesandstreptococcusagalactiaeinpresenceofcopperandzincions AT masarycheva inhibitionofthebacterialgrowthintheculturesofstreptococcuspyogenesandstreptococcusagalactiaeinpresenceofcopperandzincions AT svkisil inhibitionofthebacterialgrowthintheculturesofstreptococcuspyogenesandstreptococcusagalactiaeinpresenceofcopperandzincions AT vvanisimov inhibitionofthebacterialgrowthintheculturesofstreptococcuspyogenesandstreptococcusagalactiaeinpresenceofcopperandzincions AT avvostrov inhibitionofthebacterialgrowthintheculturesofstreptococcuspyogenesandstreptococcusagalactiaeinpresenceofcopperandzincions |