Development of a Sensitive Induction-Based Magnetic Nanoparticle Biodetection Method

Development of a Sensitive Induction-Based Magnetic Nanoparticle Biodetection Method

We developed a novel biodetection method for influenza virus based on AC magnetic susceptibility measurement techniques (the DynoMag induction technique) together with functionalized multi-core magnetic nanoparticles. The sample consisting of an incubated mixture of magnetic nanoparticles and rollin...

Full description

Bibliographic Details
Main Authors: Jakob Blomgren, Fredrik Ahrentorp, Dag Ilver, Christian Jonasson, Sobhan Sepehri, Alexei Kalaboukhov, Dag Winkler, Teresa Zardán Gómez de la Torre, Maria Strømme, Christer Johansson
Format: Article
Language:English
Published: MDPI AG 2018-11-01
Series:Nanomaterials
Subjects:
magnetic nanoparticles
magnetic biosensing
Brownian relaxation
AC susceptibility
multi-core particles
Online Access:https://www.mdpi.com/2079-4991/8/11/887
Description
Summary:We developed a novel biodetection method for influenza virus based on AC magnetic susceptibility measurement techniques (the DynoMag induction technique) together with functionalized multi-core magnetic nanoparticles. The sample consisting of an incubated mixture of magnetic nanoparticles and rolling circle amplified DNA coils is injected into a tube by a peristaltic pump. The sample is moved as a plug to the two well-balanced detection coils and the dynamic magnetic moment in each position is read over a range of excitation frequencies. The time for making a complete frequency sweep over the relaxation peak is about 5 minutes (10 Hz&#8315;10 kHz with 20 data points). The obtained standard deviation of the magnetic signal at the relaxation frequency (around 100 Hz) is equal to about 10<sup>&#8722;5</sup> (volume susceptibility SI units), which is in the same range obtained with the DynoMag system. The limit of detection with this method is found to be in the range of 1 pM.
ISSN:2079-4991

Similar Items