Development of a Sensitive Induction-Based Magnetic Nanoparticle Biodetection Method
We developed a novel biodetection method for influenza virus based on AC magnetic susceptibility measurement techniques (the DynoMag induction technique) together with functionalized multi-core magnetic nanoparticles. The sample consisting of an incubated mixture of magnetic nanoparticles and rollin...
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| Format: | Article |
| Language: | English |
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MDPI AG
2018-11-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/8/11/887 |
| _version_ | 1811263921954226176 |
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| author | Jakob Blomgren Fredrik Ahrentorp Dag Ilver Christian Jonasson Sobhan Sepehri Alexei Kalaboukhov Dag Winkler Teresa Zardán Gómez de la Torre Maria Strømme Christer Johansson |
| author_facet | Jakob Blomgren Fredrik Ahrentorp Dag Ilver Christian Jonasson Sobhan Sepehri Alexei Kalaboukhov Dag Winkler Teresa Zardán Gómez de la Torre Maria Strømme Christer Johansson |
| author_sort | Jakob Blomgren |
| collection | DOAJ |
| description | We developed a novel biodetection method for influenza virus based on AC magnetic susceptibility measurement techniques (the DynoMag induction technique) together with functionalized multi-core magnetic nanoparticles. The sample consisting of an incubated mixture of magnetic nanoparticles and rolling circle amplified DNA coils is injected into a tube by a peristaltic pump. The sample is moved as a plug to the two well-balanced detection coils and the dynamic magnetic moment in each position is read over a range of excitation frequencies. The time for making a complete frequency sweep over the relaxation peak is about 5 minutes (10 Hz⁻10 kHz with 20 data points). The obtained standard deviation of the magnetic signal at the relaxation frequency (around 100 Hz) is equal to about 10<sup>−5</sup> (volume susceptibility SI units), which is in the same range obtained with the DynoMag system. The limit of detection with this method is found to be in the range of 1 pM. |
| first_indexed | 2024-04-12T19:53:50Z |
| format | Article |
| id | doaj.art-ebc2a1f6ef9c4d6fb4397bb4f69d10a7 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-04-12T19:53:50Z |
| publishDate | 2018-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-ebc2a1f6ef9c4d6fb4397bb4f69d10a72022-12-22T03:18:45ZengMDPI AGNanomaterials2079-49912018-11-0181188710.3390/nano8110887nano8110887Development of a Sensitive Induction-Based Magnetic Nanoparticle Biodetection MethodJakob Blomgren0Fredrik Ahrentorp1Dag Ilver2Christian Jonasson3Sobhan Sepehri4Alexei Kalaboukhov5Dag Winkler6Teresa Zardán Gómez de la Torre7Maria Strømme8Christer Johansson9RISE Acreo, SE-411 33 Göteborg, SwedenRISE Acreo, SE-411 33 Göteborg, SwedenRISE Acreo, SE-411 33 Göteborg, SwedenRISE Acreo, SE-411 33 Göteborg, SwedenDepartment of Microtechnology and Nanoscience–MC2, Chalmers University of Technology, SE-412 96 Göteborg, SwedenDepartment of Microtechnology and Nanoscience–MC2, Chalmers University of Technology, SE-412 96 Göteborg, SwedenDepartment of Microtechnology and Nanoscience–MC2, Chalmers University of Technology, SE-412 96 Göteborg, SwedenDepartment of Engineering Sciences, Uppsala University, The Ångström Laboratory, SE-751 21 Uppsala, SwedenDepartment of Engineering Sciences, Uppsala University, The Ångström Laboratory, SE-751 21 Uppsala, SwedenRISE Acreo, SE-411 33 Göteborg, SwedenWe developed a novel biodetection method for influenza virus based on AC magnetic susceptibility measurement techniques (the DynoMag induction technique) together with functionalized multi-core magnetic nanoparticles. The sample consisting of an incubated mixture of magnetic nanoparticles and rolling circle amplified DNA coils is injected into a tube by a peristaltic pump. The sample is moved as a plug to the two well-balanced detection coils and the dynamic magnetic moment in each position is read over a range of excitation frequencies. The time for making a complete frequency sweep over the relaxation peak is about 5 minutes (10 Hz⁻10 kHz with 20 data points). The obtained standard deviation of the magnetic signal at the relaxation frequency (around 100 Hz) is equal to about 10<sup>−5</sup> (volume susceptibility SI units), which is in the same range obtained with the DynoMag system. The limit of detection with this method is found to be in the range of 1 pM.https://www.mdpi.com/2079-4991/8/11/887magnetic nanoparticlesmagnetic biosensingBrownian relaxationAC susceptibilitymulti-core particles |
| spellingShingle | Jakob Blomgren Fredrik Ahrentorp Dag Ilver Christian Jonasson Sobhan Sepehri Alexei Kalaboukhov Dag Winkler Teresa Zardán Gómez de la Torre Maria Strømme Christer Johansson Development of a Sensitive Induction-Based Magnetic Nanoparticle Biodetection Method Nanomaterials magnetic nanoparticles magnetic biosensing Brownian relaxation AC susceptibility multi-core particles |
| title | Development of a Sensitive Induction-Based Magnetic Nanoparticle Biodetection Method |
| title_full | Development of a Sensitive Induction-Based Magnetic Nanoparticle Biodetection Method |
| title_fullStr | Development of a Sensitive Induction-Based Magnetic Nanoparticle Biodetection Method |
| title_full_unstemmed | Development of a Sensitive Induction-Based Magnetic Nanoparticle Biodetection Method |
| title_short | Development of a Sensitive Induction-Based Magnetic Nanoparticle Biodetection Method |
| title_sort | development of a sensitive induction based magnetic nanoparticle biodetection method |
| topic | magnetic nanoparticles magnetic biosensing Brownian relaxation AC susceptibility multi-core particles |
| url | https://www.mdpi.com/2079-4991/8/11/887 |
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