<i>Tibouchina granulosa</i> Leaves Present Anti-Inflammatory Effect
The ethanol extract (EE) prepared from the leaves of <i>Tibouchina granulosa</i>, and its fraction in ethyl acetate (fEA) were evaluated concerning their capacity to reduce inflammation in different experimental models. fEA was also studied concerning its chemical constituents. EE and fE...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-11-01
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| Series: | Pharmaceuticals |
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| Online Access: | https://www.mdpi.com/1424-8247/15/12/1458 |
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| author | Carolina Carvalho Guilhon Alan Silva Minho Marc Pouliot Fabio Boylan Patricia Dias Fernandes |
| author_facet | Carolina Carvalho Guilhon Alan Silva Minho Marc Pouliot Fabio Boylan Patricia Dias Fernandes |
| author_sort | Carolina Carvalho Guilhon |
| collection | DOAJ |
| description | The ethanol extract (EE) prepared from the leaves of <i>Tibouchina granulosa</i>, and its fraction in ethyl acetate (fEA) were evaluated concerning their capacity to reduce inflammation in different experimental models. fEA was also studied concerning its chemical constituents. EE and fEA were assayed for their anti-inflammatory potential, using formalin-induced licking behavior and carrageenan-induced inflammation into the subcutaneous air pouch (SAP) models. Reduction in polymorphonuclear cells (PMN) activation was performed in freshly isolated PMN. Chromatographic analysis of fEA was performed by HPLC-DAD. Hispiduloside was isolated as the main constituent in fEA, and its quantity was estimated to be 39.3% in fEA. EE (30 mg/kg) significantly reduced the second phase of formalin-induced licking. fEA demonstrated a reduction in leukocyte migration into the SAP. EE and fEA drastically reduced cytokines (TNF-α, IL-1β, and IFN-γ), nitric oxide (NO) production, in vitro PMN migration induced by C5a and IL-8, and TNF-α and IL-1β gene expression. Taken together, our data indicate that either ethanol extract or its fEA fraction from leaves of <i>T. granulosa</i> present an anti-inflammatory effect, contributing to the pharmacological and chemical knowledge of this species and confirming the rationale behind its traditional use. |
| first_indexed | 2024-03-09T15:59:50Z |
| format | Article |
| id | doaj.art-ebc2e219a7da4bc5bb1e4ddb9be49412 |
| institution | Directory Open Access Journal |
| issn | 1424-8247 |
| language | English |
| last_indexed | 2024-03-09T15:59:50Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceuticals |
| spelling | doaj.art-ebc2e219a7da4bc5bb1e4ddb9be494122023-11-24T17:15:31ZengMDPI AGPharmaceuticals1424-82472022-11-011512145810.3390/ph15121458<i>Tibouchina granulosa</i> Leaves Present Anti-Inflammatory EffectCarolina Carvalho Guilhon0Alan Silva Minho1Marc Pouliot2Fabio Boylan3Patricia Dias Fernandes4Laboratório de Farmacologia da Dor e da Inflamação, Programa de Pesquisa em Descoberta de Fármacos, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-902, BrazilLaboratório de Farmacologia da Dor e da Inflamação, Programa de Pesquisa em Descoberta de Fármacos, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-902, BrazilCentre de Recherche du CHU de Québec, Université Laval, Québec, QC G1V 4G2, CanadaSchool of Pharmacy and Pharmaceutical Sciences and Trinity Biomedical Sciences Institute, Trinity College Dublin, 2 Dublin, IrelandLaboratório de Farmacologia da Dor e da Inflamação, Programa de Pesquisa em Descoberta de Fármacos, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-902, BrazilThe ethanol extract (EE) prepared from the leaves of <i>Tibouchina granulosa</i>, and its fraction in ethyl acetate (fEA) were evaluated concerning their capacity to reduce inflammation in different experimental models. fEA was also studied concerning its chemical constituents. EE and fEA were assayed for their anti-inflammatory potential, using formalin-induced licking behavior and carrageenan-induced inflammation into the subcutaneous air pouch (SAP) models. Reduction in polymorphonuclear cells (PMN) activation was performed in freshly isolated PMN. Chromatographic analysis of fEA was performed by HPLC-DAD. Hispiduloside was isolated as the main constituent in fEA, and its quantity was estimated to be 39.3% in fEA. EE (30 mg/kg) significantly reduced the second phase of formalin-induced licking. fEA demonstrated a reduction in leukocyte migration into the SAP. EE and fEA drastically reduced cytokines (TNF-α, IL-1β, and IFN-γ), nitric oxide (NO) production, in vitro PMN migration induced by C5a and IL-8, and TNF-α and IL-1β gene expression. Taken together, our data indicate that either ethanol extract or its fEA fraction from leaves of <i>T. granulosa</i> present an anti-inflammatory effect, contributing to the pharmacological and chemical knowledge of this species and confirming the rationale behind its traditional use.https://www.mdpi.com/1424-8247/15/12/1458<i>Tibouchina granulosa</i>anti-inflammatory activityhispidulosidecytokines |
| spellingShingle | Carolina Carvalho Guilhon Alan Silva Minho Marc Pouliot Fabio Boylan Patricia Dias Fernandes <i>Tibouchina granulosa</i> Leaves Present Anti-Inflammatory Effect Pharmaceuticals <i>Tibouchina granulosa</i> anti-inflammatory activity hispiduloside cytokines |
| title | <i>Tibouchina granulosa</i> Leaves Present Anti-Inflammatory Effect |
| title_full | <i>Tibouchina granulosa</i> Leaves Present Anti-Inflammatory Effect |
| title_fullStr | <i>Tibouchina granulosa</i> Leaves Present Anti-Inflammatory Effect |
| title_full_unstemmed | <i>Tibouchina granulosa</i> Leaves Present Anti-Inflammatory Effect |
| title_short | <i>Tibouchina granulosa</i> Leaves Present Anti-Inflammatory Effect |
| title_sort | i tibouchina granulosa i leaves present anti inflammatory effect |
| topic | <i>Tibouchina granulosa</i> anti-inflammatory activity hispiduloside cytokines |
| url | https://www.mdpi.com/1424-8247/15/12/1458 |
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