Plasma miRNA Profile in High Risk of Preterm Birth during Early and Mid-Pregnancy
In recent years evidence has been accumulated showing that miRNAs can act as potential biomarkers or targets for therapy of preterm birth, one of the most important problems in modern obstetrics. We have performed a prospective study of the miRNA profile in the plasma during the first and second tri...
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MDPI AG
2022-11-01
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| Online Access: | https://www.mdpi.com/2073-4425/13/11/2018 |
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| author | Roman A. Illarionov Olga V. Pachuliia Elena S. Vashukova Alexander A. Tkachenko Anastasia R. Maltseva Tatyana B. Postnikova Yulia A. Nasykhova Olesya N. Bespalova Andrey S. Glotov |
| author_facet | Roman A. Illarionov Olga V. Pachuliia Elena S. Vashukova Alexander A. Tkachenko Anastasia R. Maltseva Tatyana B. Postnikova Yulia A. Nasykhova Olesya N. Bespalova Andrey S. Glotov |
| author_sort | Roman A. Illarionov |
| collection | DOAJ |
| description | In recent years evidence has been accumulated showing that miRNAs can act as potential biomarkers or targets for therapy of preterm birth, one of the most important problems in modern obstetrics. We have performed a prospective study of the miRNA profile in the plasma during the first and second trimesters in pregnant women with high risk of preterm birth (<i>n</i> = 13 cases and <i>n</i> = 11 controls). For the study group plasma blood samples at 9–13 weeks before diagnosis and at 22–24 weeks after start of therapy were selected. Using high-throughput sequencing technology we detected differences in the levels of 15 miRNAs (3 upregulated—hsa-miR-122-5p, hsa-miR-34a-5p, hsa-miR-34c-5p; 12 downregulated—hsa-miR-487b-3p, hsa-miR-493-3p, hsa-miR-432-5p, hsa-miR-323b-3p, hsa-miR-369-3p, hsa-miR-134-5p, hsa-miR-431-5p, hsa-miR-485-5p, hsa-miR-382-5p, hsa-miR-369-5p, hsa-miR-485-3p, hsa-miR-127-3p) (log<sub>2</sub>(FC) ≥ 1.5; FDR ≤ 0.05) during the first trimester compared with the control (non-high-risk of preterm birth pregnant women). All downregulated miRNAs in the first trimester from the placenta-specific C14MC cluster. During the second trimester no differentially expressed miRNAs were found. Our results suggest that the miRNA profile in plasma during early pregnancy may predict a high risk of preterm birth, which is important in preventing gestational problems as early as possible. |
| first_indexed | 2024-03-09T19:04:00Z |
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| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-09T19:04:00Z |
| publishDate | 2022-11-01 |
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| spelling | doaj.art-ebc31dec10374b3a973466aeee500e3e2023-11-24T04:48:30ZengMDPI AGGenes2073-44252022-11-011311201810.3390/genes13112018Plasma miRNA Profile in High Risk of Preterm Birth during Early and Mid-PregnancyRoman A. Illarionov0Olga V. Pachuliia1Elena S. Vashukova2Alexander A. Tkachenko3Anastasia R. Maltseva4Tatyana B. Postnikova5Yulia A. Nasykhova6Olesya N. Bespalova7Andrey S. Glotov8Department of Genomic Medicine, D.O. Ott Research Institute for Obstetrics, Gynecology, and Reproduction, St. Petersburg 199034, RussiaDepartment of Genomic Medicine, D.O. Ott Research Institute for Obstetrics, Gynecology, and Reproduction, St. Petersburg 199034, RussiaDepartment of Genomic Medicine, D.O. Ott Research Institute for Obstetrics, Gynecology, and Reproduction, St. Petersburg 199034, RussiaDepartment of Genomic Medicine, D.O. Ott Research Institute for Obstetrics, Gynecology, and Reproduction, St. Petersburg 199034, RussiaDepartment of Genomic Medicine, D.O. Ott Research Institute for Obstetrics, Gynecology, and Reproduction, St. Petersburg 199034, RussiaDepartment of Genomic Medicine, D.O. Ott Research Institute for Obstetrics, Gynecology, and Reproduction, St. Petersburg 199034, RussiaDepartment of Genomic Medicine, D.O. Ott Research Institute for Obstetrics, Gynecology, and Reproduction, St. Petersburg 199034, RussiaDepartment of Genomic Medicine, D.O. Ott Research Institute for Obstetrics, Gynecology, and Reproduction, St. Petersburg 199034, RussiaDepartment of Genomic Medicine, D.O. Ott Research Institute for Obstetrics, Gynecology, and Reproduction, St. Petersburg 199034, RussiaIn recent years evidence has been accumulated showing that miRNAs can act as potential biomarkers or targets for therapy of preterm birth, one of the most important problems in modern obstetrics. We have performed a prospective study of the miRNA profile in the plasma during the first and second trimesters in pregnant women with high risk of preterm birth (<i>n</i> = 13 cases and <i>n</i> = 11 controls). For the study group plasma blood samples at 9–13 weeks before diagnosis and at 22–24 weeks after start of therapy were selected. Using high-throughput sequencing technology we detected differences in the levels of 15 miRNAs (3 upregulated—hsa-miR-122-5p, hsa-miR-34a-5p, hsa-miR-34c-5p; 12 downregulated—hsa-miR-487b-3p, hsa-miR-493-3p, hsa-miR-432-5p, hsa-miR-323b-3p, hsa-miR-369-3p, hsa-miR-134-5p, hsa-miR-431-5p, hsa-miR-485-5p, hsa-miR-382-5p, hsa-miR-369-5p, hsa-miR-485-3p, hsa-miR-127-3p) (log<sub>2</sub>(FC) ≥ 1.5; FDR ≤ 0.05) during the first trimester compared with the control (non-high-risk of preterm birth pregnant women). All downregulated miRNAs in the first trimester from the placenta-specific C14MC cluster. During the second trimester no differentially expressed miRNAs were found. Our results suggest that the miRNA profile in plasma during early pregnancy may predict a high risk of preterm birth, which is important in preventing gestational problems as early as possible.https://www.mdpi.com/2073-4425/13/11/2018miRNApregnancyhigh risk of preterm birthblood plasmahigh-throughput sequencing |
| spellingShingle | Roman A. Illarionov Olga V. Pachuliia Elena S. Vashukova Alexander A. Tkachenko Anastasia R. Maltseva Tatyana B. Postnikova Yulia A. Nasykhova Olesya N. Bespalova Andrey S. Glotov Plasma miRNA Profile in High Risk of Preterm Birth during Early and Mid-Pregnancy Genes miRNA pregnancy high risk of preterm birth blood plasma high-throughput sequencing |
| title | Plasma miRNA Profile in High Risk of Preterm Birth during Early and Mid-Pregnancy |
| title_full | Plasma miRNA Profile in High Risk of Preterm Birth during Early and Mid-Pregnancy |
| title_fullStr | Plasma miRNA Profile in High Risk of Preterm Birth during Early and Mid-Pregnancy |
| title_full_unstemmed | Plasma miRNA Profile in High Risk of Preterm Birth during Early and Mid-Pregnancy |
| title_short | Plasma miRNA Profile in High Risk of Preterm Birth during Early and Mid-Pregnancy |
| title_sort | plasma mirna profile in high risk of preterm birth during early and mid pregnancy |
| topic | miRNA pregnancy high risk of preterm birth blood plasma high-throughput sequencing |
| url | https://www.mdpi.com/2073-4425/13/11/2018 |
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