The inhibitory role of effective microorganisms on the growth of pathogenic bacteria
This study was conducted to evaluate the efficacy of Effective Microorganisms (EM1<sup>®</sup>) for inhibiting the growth of some pathogenic bacteria <em>Staphylococcus aureus</em> and <em>E. coli</em> were used in this study and isolated from pathological conditi...
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| Format: | Article |
| Language: | Arabic |
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University of Mosul, College of Veterinary Medicine
2020-01-01
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| Series: | Iraqi Journal of Veterinary Sciences |
| Subjects: | |
| Online Access: | https://vetmedmosul.com/article_164345_5320c01f2c5a1beb7f9a23a8b50bf677.pdf |
| _version_ | 1811273520672407552 |
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| author | Mohammad A. Hamad Saba A. Hussein Ebtehal N. Mahmmoud Ammar M. Al-AAlim |
| author_facet | Mohammad A. Hamad Saba A. Hussein Ebtehal N. Mahmmoud Ammar M. Al-AAlim |
| author_sort | Mohammad A. Hamad |
| collection | DOAJ |
| description | This study was conducted to evaluate the efficacy of Effective Microorganisms (EM1<sup>®</sup>) for inhibiting the growth of some pathogenic bacteria <em>Staphylococcus aureus</em> and <em>E. coli</em> were used in this study and isolated from pathological conditions. These bacteria were diagnosed in laboratory of microbiology, College of Veterinary Medicine, University of Mosul. The colonies that taken from blood agar were 5-7 and cultured in the nutrient broth and incubated at 37 ºC for 24 hours. Bacterial growth was calibrated with the second tube of the McFarland tubes 0.5%. Several concentrations of EM product were prepared 1, 0.5, 0.25 and 0.125%. Decimal dilutions were done for each concentration of EM product with bacterial suspension, except control group was done for bacterial suspension with nutrient broth. The bacterial count was done on nutrient agar, milk agar and EMB agar. The results of this study showed that the product of EM1<sup>®</sup> within concentrations 0.5-1% was highly efficient in inhibiting the growth of pathogenic bacteria under study. The bacterial count of both <em>S. aureus</em> and <em>E. coli</em> was 54x10<sup>7</sup> and 52x10<sup>7</sup> CFU/ ml respectively at 1% EM1<sup>®</sup>, and 67x10<sup>7</sup> and 86x10<sup>7</sup> CFU/ ml respectively at 0.5%, while the counting of the control group was 42x10<sup>9</sup> and 67x10<sup>9</sup> CFU/ ml respectively. This study concluded that EM1<sup>®</sup> at low concentrations have a clear role in inhibiting the growth of pathogenic bacteria, particularly<em> S. aureus</em> and<em> E. coli</em>. |
| first_indexed | 2024-04-12T23:00:43Z |
| format | Article |
| id | doaj.art-ebc7cfdc225b464c92f6441543b85adc |
| institution | Directory Open Access Journal |
| issn | 1607-3894 2071-1255 |
| language | Arabic |
| last_indexed | 2024-04-12T23:00:43Z |
| publishDate | 2020-01-01 |
| publisher | University of Mosul, College of Veterinary Medicine |
| record_format | Article |
| series | Iraqi Journal of Veterinary Sciences |
| spelling | doaj.art-ebc7cfdc225b464c92f6441543b85adc2022-12-22T03:13:04ZaraUniversity of Mosul, College of Veterinary MedicineIraqi Journal of Veterinary Sciences1607-38942071-12552020-01-0134115315810.33899/ijvs.2019.125653.1123164345The inhibitory role of effective microorganisms on the growth of pathogenic bacteriaMohammad A. Hamad0Saba A. Hussein1Ebtehal N. Mahmmoud2Ammar M. Al-AAlim3Department of Microbiology, College of Veterinary Medicine. University of Mosul. Mosul city, IraqDepartment of Microbiology, College of Veterinary Medicine, University of Mosul, Mosul, IraqDepartment of Microbiology, College of Veterinary Medicine, University of Mosul, Mosul, IraqDepartment of Microbiology, College of Veterinary Medicine, University of Mosul, Mosul, IraqThis study was conducted to evaluate the efficacy of Effective Microorganisms (EM1<sup>®</sup>) for inhibiting the growth of some pathogenic bacteria <em>Staphylococcus aureus</em> and <em>E. coli</em> were used in this study and isolated from pathological conditions. These bacteria were diagnosed in laboratory of microbiology, College of Veterinary Medicine, University of Mosul. The colonies that taken from blood agar were 5-7 and cultured in the nutrient broth and incubated at 37 ºC for 24 hours. Bacterial growth was calibrated with the second tube of the McFarland tubes 0.5%. Several concentrations of EM product were prepared 1, 0.5, 0.25 and 0.125%. Decimal dilutions were done for each concentration of EM product with bacterial suspension, except control group was done for bacterial suspension with nutrient broth. The bacterial count was done on nutrient agar, milk agar and EMB agar. The results of this study showed that the product of EM1<sup>®</sup> within concentrations 0.5-1% was highly efficient in inhibiting the growth of pathogenic bacteria under study. The bacterial count of both <em>S. aureus</em> and <em>E. coli</em> was 54x10<sup>7</sup> and 52x10<sup>7</sup> CFU/ ml respectively at 1% EM1<sup>®</sup>, and 67x10<sup>7</sup> and 86x10<sup>7</sup> CFU/ ml respectively at 0.5%, while the counting of the control group was 42x10<sup>9</sup> and 67x10<sup>9</sup> CFU/ ml respectively. This study concluded that EM1<sup>®</sup> at low concentrations have a clear role in inhibiting the growth of pathogenic bacteria, particularly<em> S. aureus</em> and<em> E. coli</em>.https://vetmedmosul.com/article_164345_5320c01f2c5a1beb7f9a23a8b50bf677.pdfbacterial counts. aureuse. coli |
| spellingShingle | Mohammad A. Hamad Saba A. Hussein Ebtehal N. Mahmmoud Ammar M. Al-AAlim The inhibitory role of effective microorganisms on the growth of pathogenic bacteria Iraqi Journal of Veterinary Sciences bacterial count s. aureus e. coli |
| title | The inhibitory role of effective microorganisms on the growth of pathogenic bacteria |
| title_full | The inhibitory role of effective microorganisms on the growth of pathogenic bacteria |
| title_fullStr | The inhibitory role of effective microorganisms on the growth of pathogenic bacteria |
| title_full_unstemmed | The inhibitory role of effective microorganisms on the growth of pathogenic bacteria |
| title_short | The inhibitory role of effective microorganisms on the growth of pathogenic bacteria |
| title_sort | inhibitory role of effective microorganisms on the growth of pathogenic bacteria |
| topic | bacterial count s. aureus e. coli |
| url | https://vetmedmosul.com/article_164345_5320c01f2c5a1beb7f9a23a8b50bf677.pdf |
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