Early Responses of Brassica oleracea Roots to Zinc Supply Under Sufficient and Sub-Optimal Phosphorus Supply
Shoot zinc (Zn) concentration in Brassica oleracea is affected by soil Zn and phosphorus (P) supply. Most problematic is the negative impact of P fertilizers on Zn concentrations in crops, which makes balancing yield and mineral quality challenging. To evaluate early molecular mechanisms involved in...
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Frontiers Media S.A.
2020-01-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fpls.2019.01645/full |
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| author | Paula Pongrac Paula Pongrac Sina Fischer Jacqueline A. Thompson Gladys Wright Philip J. White Philip J. White |
| author_facet | Paula Pongrac Paula Pongrac Sina Fischer Jacqueline A. Thompson Gladys Wright Philip J. White Philip J. White |
| author_sort | Paula Pongrac |
| collection | DOAJ |
| description | Shoot zinc (Zn) concentration in Brassica oleracea is affected by soil Zn and phosphorus (P) supply. Most problematic is the negative impact of P fertilizers on Zn concentrations in crops, which makes balancing yield and mineral quality challenging. To evaluate early molecular mechanisms involved in the accumulation of large shoot Zn concentrations regardless of the P supply, two B. oleracea accessions differing in root architecture and root exudates were grown hydroponically for two weeks with different combinations of P and Zn supply. Ionome profiling and deep RNA sequencing of roots revealed interactions of P and Zn in planta, without apparent phenotypic effects. In addition, increasing P supply did not reduce tissue Zn concentration. Substantial changes in gene expression in response to different P and/or Zn supplies in roots of both accessions ensured nutritionally sufficient P and Zn uptake. Numerous genes were differentially expressed after changing Zn or P supply and most of them were unique to only one accession, highlighting their different strategies in achieving nutrient sufficiency. Thus, different gene networks responded to the changing P and Zn supply in the two accessions. Additionally, enrichment analysis of gene ontology classes revealed that genes involved in lipid metabolism, response to starvation, and anion transport mechanisms were most responsive to differences in P and Zn supply in both accessions. The results agreed with previously studies demonstrating alterations in P and Zn transport and phospholipid metabolism in response to reduced P and Zn supply. It is anticipated that improved knowledge of genes responsive to P or Zn supply will help illuminate the roles in uptake and accumulation of P and Zn and might identify candidate genes for breeding high-yield-high-Zn brassicas. |
| first_indexed | 2024-12-11T18:26:25Z |
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| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-11T18:26:25Z |
| publishDate | 2020-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-ebc8209250944b5a85e72cca6dc517042022-12-22T00:55:05ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2020-01-011010.3389/fpls.2019.01645457437Early Responses of Brassica oleracea Roots to Zinc Supply Under Sufficient and Sub-Optimal Phosphorus SupplyPaula Pongrac0Paula Pongrac1Sina Fischer2Jacqueline A. Thompson3Gladys Wright4Philip J. White5Philip J. White6Ecological Science Group, The James Hutton Institute, Dundee, United KingdomLow and Medium Energy Physics, Jožef Stefan Institute, Ljubljana, SloveniaFuture Food Beacon of Excellence and School of Biosciences, University of Nottingham, Loughborough, United KingdomEcological Science Group, The James Hutton Institute, Dundee, United KingdomEcological Science Group, The James Hutton Institute, Dundee, United KingdomEcological Science Group, The James Hutton Institute, Dundee, United KingdomDistinguished Scientist Fellowship Program, King Saud University, Riyadh, Saudi ArabiaShoot zinc (Zn) concentration in Brassica oleracea is affected by soil Zn and phosphorus (P) supply. Most problematic is the negative impact of P fertilizers on Zn concentrations in crops, which makes balancing yield and mineral quality challenging. To evaluate early molecular mechanisms involved in the accumulation of large shoot Zn concentrations regardless of the P supply, two B. oleracea accessions differing in root architecture and root exudates were grown hydroponically for two weeks with different combinations of P and Zn supply. Ionome profiling and deep RNA sequencing of roots revealed interactions of P and Zn in planta, without apparent phenotypic effects. In addition, increasing P supply did not reduce tissue Zn concentration. Substantial changes in gene expression in response to different P and/or Zn supplies in roots of both accessions ensured nutritionally sufficient P and Zn uptake. Numerous genes were differentially expressed after changing Zn or P supply and most of them were unique to only one accession, highlighting their different strategies in achieving nutrient sufficiency. Thus, different gene networks responded to the changing P and Zn supply in the two accessions. Additionally, enrichment analysis of gene ontology classes revealed that genes involved in lipid metabolism, response to starvation, and anion transport mechanisms were most responsive to differences in P and Zn supply in both accessions. The results agreed with previously studies demonstrating alterations in P and Zn transport and phospholipid metabolism in response to reduced P and Zn supply. It is anticipated that improved knowledge of genes responsive to P or Zn supply will help illuminate the roles in uptake and accumulation of P and Zn and might identify candidate genes for breeding high-yield-high-Zn brassicas.https://www.frontiersin.org/article/10.3389/fpls.2019.01645/fullmineral nutritionkalebroccolielement interactionRNAseqgene ontology |
| spellingShingle | Paula Pongrac Paula Pongrac Sina Fischer Jacqueline A. Thompson Gladys Wright Philip J. White Philip J. White Early Responses of Brassica oleracea Roots to Zinc Supply Under Sufficient and Sub-Optimal Phosphorus Supply Frontiers in Plant Science mineral nutrition kale broccoli element interaction RNAseq gene ontology |
| title | Early Responses of Brassica oleracea Roots to Zinc Supply Under Sufficient and Sub-Optimal Phosphorus Supply |
| title_full | Early Responses of Brassica oleracea Roots to Zinc Supply Under Sufficient and Sub-Optimal Phosphorus Supply |
| title_fullStr | Early Responses of Brassica oleracea Roots to Zinc Supply Under Sufficient and Sub-Optimal Phosphorus Supply |
| title_full_unstemmed | Early Responses of Brassica oleracea Roots to Zinc Supply Under Sufficient and Sub-Optimal Phosphorus Supply |
| title_short | Early Responses of Brassica oleracea Roots to Zinc Supply Under Sufficient and Sub-Optimal Phosphorus Supply |
| title_sort | early responses of brassica oleracea roots to zinc supply under sufficient and sub optimal phosphorus supply |
| topic | mineral nutrition kale broccoli element interaction RNAseq gene ontology |
| url | https://www.frontiersin.org/article/10.3389/fpls.2019.01645/full |
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