Embryo Culture, In Vitro Propagation, and Molecular Identification for Advanced Olive Breeding Programs
The high biodiversity of the olive tree is an important opportunity to develop sustainable plans to control <i>Xylella</i> <i>fastidiosa</i> (<i>Xf</i>) through breeding programs. Olive tree breeding activities have been limited due to various features of this spe...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-12-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/8/1/36 |
| _version_ | 1797493607225098240 |
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| author | Vito Montilon Leonardo Susca Oriana Potere Vincenzo Roseti Antonia Campanale Antonia Saponari Cinzia Montemurro Valentina Fanelli Pasquale Venerito Giovanna Bottalico |
| author_facet | Vito Montilon Leonardo Susca Oriana Potere Vincenzo Roseti Antonia Campanale Antonia Saponari Cinzia Montemurro Valentina Fanelli Pasquale Venerito Giovanna Bottalico |
| author_sort | Vito Montilon |
| collection | DOAJ |
| description | The high biodiversity of the olive tree is an important opportunity to develop sustainable plans to control <i>Xylella</i> <i>fastidiosa</i> (<i>Xf</i>) through breeding programs. Olive tree breeding activities have been limited due to various features of this species including the long time required for seed germination caused by the inhibition effect of the woody endocarp, the seed integument, and the endosperm. Starting from F<sub>1</sub> seeds by cross-breeding, the embryo culture was compared with traditional seed germination, evaluating the effectiveness of in vitro multiplication of the plantlets for large-scale production. The isolated embryos were established on a new medium based on Rugini ‘84 macroelements, Murashige & Skoog ‘62 microelements, with Nitsch J. P. & Nitsch C. ‘69 vitamine and subcultured on Leva MSM modified. The results obtained confirmed that in vitro culture of olive embryos is a valid tool for increasing the percentage and speed of germination, helping to reduce the time of the olive breeding programs, offering the possibility to effectively propagate plantlets for further experiments. |
| first_indexed | 2024-03-10T01:22:27Z |
| format | Article |
| id | doaj.art-ebca24e8dbd246309454d95015e7ce3b |
| institution | Directory Open Access Journal |
| issn | 2311-7524 |
| language | English |
| last_indexed | 2024-03-10T01:22:27Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Horticulturae |
| spelling | doaj.art-ebca24e8dbd246309454d95015e7ce3b2023-11-23T13:57:35ZengMDPI AGHorticulturae2311-75242021-12-01813610.3390/horticulturae8010036Embryo Culture, In Vitro Propagation, and Molecular Identification for Advanced Olive Breeding ProgramsVito Montilon0Leonardo Susca1Oriana Potere2Vincenzo Roseti3Antonia Campanale4Antonia Saponari5Cinzia Montemurro6Valentina Fanelli7Pasquale Venerito8Giovanna Bottalico9Department of Soil, Plant and Food Sciences (DiSSPA), University of Bari Aldo Moro, 70126 Bari, ItalyDepartment of Soil, Plant and Food Sciences (DiSSPA), University of Bari Aldo Moro, 70126 Bari, ItalyDepartment of Soil, Plant and Food Sciences (DiSSPA), University of Bari Aldo Moro, 70126 Bari, ItalyDepartment of Soil, Plant and Food Sciences (DiSSPA), University of Bari Aldo Moro, 70126 Bari, ItalyInstitute for Sustainable Plant Protection-IPSP—CNR, 70126 Bari, ItalyCentre for Research, Experimentation and Education in Agriculture (CRSFA) “Basile Caramia”, 70010 Locorotondo, ItalyDepartment of Soil, Plant and Food Sciences (DiSSPA), University of Bari Aldo Moro, 70126 Bari, ItalyDepartment of Soil, Plant and Food Sciences (DiSSPA), University of Bari Aldo Moro, 70126 Bari, ItalyCentre for Research, Experimentation and Education in Agriculture (CRSFA) “Basile Caramia”, 70010 Locorotondo, ItalyDepartment of Soil, Plant and Food Sciences (DiSSPA), University of Bari Aldo Moro, 70126 Bari, ItalyThe high biodiversity of the olive tree is an important opportunity to develop sustainable plans to control <i>Xylella</i> <i>fastidiosa</i> (<i>Xf</i>) through breeding programs. Olive tree breeding activities have been limited due to various features of this species including the long time required for seed germination caused by the inhibition effect of the woody endocarp, the seed integument, and the endosperm. Starting from F<sub>1</sub> seeds by cross-breeding, the embryo culture was compared with traditional seed germination, evaluating the effectiveness of in vitro multiplication of the plantlets for large-scale production. The isolated embryos were established on a new medium based on Rugini ‘84 macroelements, Murashige & Skoog ‘62 microelements, with Nitsch J. P. & Nitsch C. ‘69 vitamine and subcultured on Leva MSM modified. The results obtained confirmed that in vitro culture of olive embryos is a valid tool for increasing the percentage and speed of germination, helping to reduce the time of the olive breeding programs, offering the possibility to effectively propagate plantlets for further experiments.https://www.mdpi.com/2311-7524/8/1/36seed germinationembryo cultureclonal propagationolive treeSSR markersintervarietal crossbreeding |
| spellingShingle | Vito Montilon Leonardo Susca Oriana Potere Vincenzo Roseti Antonia Campanale Antonia Saponari Cinzia Montemurro Valentina Fanelli Pasquale Venerito Giovanna Bottalico Embryo Culture, In Vitro Propagation, and Molecular Identification for Advanced Olive Breeding Programs Horticulturae seed germination embryo culture clonal propagation olive tree SSR markers intervarietal crossbreeding |
| title | Embryo Culture, In Vitro Propagation, and Molecular Identification for Advanced Olive Breeding Programs |
| title_full | Embryo Culture, In Vitro Propagation, and Molecular Identification for Advanced Olive Breeding Programs |
| title_fullStr | Embryo Culture, In Vitro Propagation, and Molecular Identification for Advanced Olive Breeding Programs |
| title_full_unstemmed | Embryo Culture, In Vitro Propagation, and Molecular Identification for Advanced Olive Breeding Programs |
| title_short | Embryo Culture, In Vitro Propagation, and Molecular Identification for Advanced Olive Breeding Programs |
| title_sort | embryo culture in vitro propagation and molecular identification for advanced olive breeding programs |
| topic | seed germination embryo culture clonal propagation olive tree SSR markers intervarietal crossbreeding |
| url | https://www.mdpi.com/2311-7524/8/1/36 |
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