Assessment of Hepatoprotective Effect of Chokeberry Juice in Rats Treated Chronically with Carbon Tetrachloride
The aim of this study was to compare the protective effects of chokeberry juice and silymarin against chemical-induced liver fibrosis in rats. Liver fibrosis was induced by CCl<sub>4</sub> administered two days a week for six weeks. Two groups of rats were co-treated with chokeberry juic...
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MDPI AG
2020-03-01
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| Serier: | Molecules |
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| Online adgang: | https://www.mdpi.com/1420-3049/25/6/1268 |
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| author | Hanna Piotrowska-Kempisty Michał Nowicki Jadwiga Jodynis-Liebert Monika Kurpik Małgorzata Ewertowska Teresa Adamska Jan Oszmiański Małgorzata Kujawska |
| author_facet | Hanna Piotrowska-Kempisty Michał Nowicki Jadwiga Jodynis-Liebert Monika Kurpik Małgorzata Ewertowska Teresa Adamska Jan Oszmiański Małgorzata Kujawska |
| author_sort | Hanna Piotrowska-Kempisty |
| collection | DOAJ |
| description | The aim of this study was to compare the protective effects of chokeberry juice and silymarin against chemical-induced liver fibrosis in rats. Liver fibrosis was induced by CCl<sub>4</sub> administered two days a week for six weeks. Two groups of rats were co-treated with chokeberry juice, 10 mL/kg/day. or silymarin as a positive control, 100 mg/kg/day for six weeks. Hepatic lipid peroxidation was suppressed by 50% and the activity of hepatic antioxidant enzymes was increased by 19%−173% in rats co-treated with CCl<sub>4</sub> and substances tested as compared to rats administered CCl<sub>4</sub> alone. Hepatic hydroxyproline was decreased by 24% only in rats treated with silymarin. The messenger RNA (mRNA) expression levels of fibrosis-related molecules, procollagen I, α-SMA, TIMP-1, TGFβ, and TNFα, which were significantly increased in the liver of CCl<sub>4</sub>-treated rats, were not modulated by substances tested. Histological evaluation revealed a slight protective effect of silymarin against fibrosis. However, in CCl<sub>4</sub> + chokeberry-treated rats, the density of vacuolated hepatocytes was significantly lower than that in silymarin administered animals. Chokeberry juice did not demonstrate an antifibrotic effect in the applied experimental model of fibrosis, and the effect of the known antifibrotic agent, silymarin, was very limited. |
| first_indexed | 2024-12-11T20:19:06Z |
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| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-11T20:19:06Z |
| publishDate | 2020-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-ebccb1efd51a42feb4a9fe9b81d77c972022-12-22T00:52:07ZengMDPI AGMolecules1420-30492020-03-01256126810.3390/molecules25061268molecules25061268Assessment of Hepatoprotective Effect of Chokeberry Juice in Rats Treated Chronically with Carbon TetrachlorideHanna Piotrowska-Kempisty0Michał Nowicki1Jadwiga Jodynis-Liebert2Monika Kurpik3Małgorzata Ewertowska4Teresa Adamska5Jan Oszmiański6Małgorzata Kujawska7Department of Toxicology, Poznan University of Medical Sciences, 60-631 Poznań, PolandDepartment of Histology and Embryology, Poznan University of Medical Sciences, 60-781 Poznan, PolandDepartment of Toxicology, Poznan University of Medical Sciences, 60-631 Poznań, PolandDepartment of Toxicology, Poznan University of Medical Sciences, 60-631 Poznań, PolandDepartment of Toxicology, Poznan University of Medical Sciences, 60-631 Poznań, PolandDepartment of Toxicology, Poznan University of Medical Sciences, 60-631 Poznań, PolandDepartment of Fruit, Vegetable and Cereal Technology, Environmental and Life Science University, 51-630 Wrocław, PolandDepartment of Toxicology, Poznan University of Medical Sciences, 60-631 Poznań, PolandThe aim of this study was to compare the protective effects of chokeberry juice and silymarin against chemical-induced liver fibrosis in rats. Liver fibrosis was induced by CCl<sub>4</sub> administered two days a week for six weeks. Two groups of rats were co-treated with chokeberry juice, 10 mL/kg/day. or silymarin as a positive control, 100 mg/kg/day for six weeks. Hepatic lipid peroxidation was suppressed by 50% and the activity of hepatic antioxidant enzymes was increased by 19%−173% in rats co-treated with CCl<sub>4</sub> and substances tested as compared to rats administered CCl<sub>4</sub> alone. Hepatic hydroxyproline was decreased by 24% only in rats treated with silymarin. The messenger RNA (mRNA) expression levels of fibrosis-related molecules, procollagen I, α-SMA, TIMP-1, TGFβ, and TNFα, which were significantly increased in the liver of CCl<sub>4</sub>-treated rats, were not modulated by substances tested. Histological evaluation revealed a slight protective effect of silymarin against fibrosis. However, in CCl<sub>4</sub> + chokeberry-treated rats, the density of vacuolated hepatocytes was significantly lower than that in silymarin administered animals. Chokeberry juice did not demonstrate an antifibrotic effect in the applied experimental model of fibrosis, and the effect of the known antifibrotic agent, silymarin, was very limited.https://www.mdpi.com/1420-3049/25/6/1268chokeberryliver fibrosisoxidative stresssilymarin |
| spellingShingle | Hanna Piotrowska-Kempisty Michał Nowicki Jadwiga Jodynis-Liebert Monika Kurpik Małgorzata Ewertowska Teresa Adamska Jan Oszmiański Małgorzata Kujawska Assessment of Hepatoprotective Effect of Chokeberry Juice in Rats Treated Chronically with Carbon Tetrachloride Molecules chokeberry liver fibrosis oxidative stress silymarin |
| title | Assessment of Hepatoprotective Effect of Chokeberry Juice in Rats Treated Chronically with Carbon Tetrachloride |
| title_full | Assessment of Hepatoprotective Effect of Chokeberry Juice in Rats Treated Chronically with Carbon Tetrachloride |
| title_fullStr | Assessment of Hepatoprotective Effect of Chokeberry Juice in Rats Treated Chronically with Carbon Tetrachloride |
| title_full_unstemmed | Assessment of Hepatoprotective Effect of Chokeberry Juice in Rats Treated Chronically with Carbon Tetrachloride |
| title_short | Assessment of Hepatoprotective Effect of Chokeberry Juice in Rats Treated Chronically with Carbon Tetrachloride |
| title_sort | assessment of hepatoprotective effect of chokeberry juice in rats treated chronically with carbon tetrachloride |
| topic | chokeberry liver fibrosis oxidative stress silymarin |
| url | https://www.mdpi.com/1420-3049/25/6/1268 |
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