Some traits of Verotoxin-producing strains of Escherichia coli isolated from cattle
Verotoxin-producing Esherichia coli (VTEC) is one of six pathogenicity groups of Eschericha coli. The reservoir for VTEC is the intestinal tract of domestic animals, primarily ruminants. Investigations in our geographic region have also demonstrated that domestic animals are a significant VTEC reser...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Faculty of Veterinary Medicine, Belgrade
2010-01-01
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| Series: | Veterinarski Glasnik |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0350-2457/2010/0350-24571006339L.pdf |
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| author | Lazić Srđan Stajković Novica Dimić Bane Lepšanović Zorica Krstić Milena Čekanac Radovan Mladenović Jovan Jadranin Žarko Radaković Sonja Rađen Slavica |
| author_facet | Lazić Srđan Stajković Novica Dimić Bane Lepšanović Zorica Krstić Milena Čekanac Radovan Mladenović Jovan Jadranin Žarko Radaković Sonja Rađen Slavica |
| author_sort | Lazić Srđan |
| collection | DOAJ |
| description | Verotoxin-producing Esherichia coli (VTEC) is one of six pathogenicity groups of Eschericha coli. The reservoir for VTEC is the intestinal tract of domestic animals, primarily ruminants. Investigations in our geographic region have also demonstrated that domestic animals are a significant VTEC reservoir. In spite of this, sporadic diseases in humans caused by these agents are rare, and no epidemics have been registered so far. The question is, therefore, what percentage of VTEC isolated in our region have the characteristics of enterohaemorrhagic E. coli (EHEC) which cause intestinal diseases in humans. The aim of this study was to test the isolated bovine strains for some phenotypic and genotypic traits and to determine the percent of strains that belong to EHEC. A total of 105 VTEC strains isolated from cattle were tested for the presence of verotoxin (vtx) genes by PCR (polymerase chain reaction) method. All of them possessed one or more vtx genes. In accordance with our investigations, only four (3.8%) strains, of a total of 105 VTEC strains belong to groups of EHEC. It may be concluded that the majority of strains isolated from cattle in this part of the world do not have phenotypic traits typical for EHEC. Therefore human VTEC associated diseases in Serbia are rare, despite the fact that domestic animals frequently harbor VTEC. |
| first_indexed | 2024-12-20T15:39:01Z |
| format | Article |
| id | doaj.art-ebccea2c30cb4910b0028a63dcf623eb |
| institution | Directory Open Access Journal |
| issn | 0350-2457 2406-0771 |
| language | English |
| last_indexed | 2024-12-20T15:39:01Z |
| publishDate | 2010-01-01 |
| publisher | Faculty of Veterinary Medicine, Belgrade |
| record_format | Article |
| series | Veterinarski Glasnik |
| spelling | doaj.art-ebccea2c30cb4910b0028a63dcf623eb2022-12-21T19:35:17ZengFaculty of Veterinary Medicine, BelgradeVeterinarski Glasnik0350-24572406-07712010-01-01645-633934810.2298/VETGL1006339L0350-24571006339LSome traits of Verotoxin-producing strains of Escherichia coli isolated from cattleLazić Srđan0Stajković Novica1Dimić Bane2Lepšanović Zorica3Krstić Milena4Čekanac Radovan5Mladenović Jovan6Jadranin Žarko7Radaković Sonja8Rađen Slavica9Institut za epidemiologiju, Vojno medicinska akademija, Beograd%SR71-02Institut za epidemiologiju, Vojno medicinska akademija, Beograd%SR71-02Veterinarska stanica, Beograd%SR61-01Institut za epidemiologiju, Vojno medicinska akademija, Beograd%SR71-02Institut za epidemiologiju, Vojno medicinska akademija, Beograd%SR71-02Institut za epidemiologiju, Vojno medicinska akademija, Beograd%SR71-02Institut za epidemiologiju, Vojno medicinska akademija, Beograd%SR71-02Institut za epidemiologiju, Vojno medicinska akademija, Beograd%SR71-02Institut za higijenu, Vojno medicinska akademija, Beograd%SR71-02Institut za higijenu, Vojno medicinska akademija, Beograd%SR71-02Verotoxin-producing Esherichia coli (VTEC) is one of six pathogenicity groups of Eschericha coli. The reservoir for VTEC is the intestinal tract of domestic animals, primarily ruminants. Investigations in our geographic region have also demonstrated that domestic animals are a significant VTEC reservoir. In spite of this, sporadic diseases in humans caused by these agents are rare, and no epidemics have been registered so far. The question is, therefore, what percentage of VTEC isolated in our region have the characteristics of enterohaemorrhagic E. coli (EHEC) which cause intestinal diseases in humans. The aim of this study was to test the isolated bovine strains for some phenotypic and genotypic traits and to determine the percent of strains that belong to EHEC. A total of 105 VTEC strains isolated from cattle were tested for the presence of verotoxin (vtx) genes by PCR (polymerase chain reaction) method. All of them possessed one or more vtx genes. In accordance with our investigations, only four (3.8%) strains, of a total of 105 VTEC strains belong to groups of EHEC. It may be concluded that the majority of strains isolated from cattle in this part of the world do not have phenotypic traits typical for EHEC. Therefore human VTEC associated diseases in Serbia are rare, despite the fact that domestic animals frequently harbor VTEC.http://www.doiserbia.nb.rs/img/doi/0350-2457/2010/0350-24571006339L.pdfVerotoxinEscherichia coliVTECEHECPCRcattle |
| spellingShingle | Lazić Srđan Stajković Novica Dimić Bane Lepšanović Zorica Krstić Milena Čekanac Radovan Mladenović Jovan Jadranin Žarko Radaković Sonja Rađen Slavica Some traits of Verotoxin-producing strains of Escherichia coli isolated from cattle Veterinarski Glasnik Verotoxin Escherichia coli VTEC EHEC PCR cattle |
| title | Some traits of Verotoxin-producing strains of Escherichia coli isolated from cattle |
| title_full | Some traits of Verotoxin-producing strains of Escherichia coli isolated from cattle |
| title_fullStr | Some traits of Verotoxin-producing strains of Escherichia coli isolated from cattle |
| title_full_unstemmed | Some traits of Verotoxin-producing strains of Escherichia coli isolated from cattle |
| title_short | Some traits of Verotoxin-producing strains of Escherichia coli isolated from cattle |
| title_sort | some traits of verotoxin producing strains of escherichia coli isolated from cattle |
| topic | Verotoxin Escherichia coli VTEC EHEC PCR cattle |
| url | http://www.doiserbia.nb.rs/img/doi/0350-2457/2010/0350-24571006339L.pdf |
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