Valorization of Taiwan's Citrus depressa Hayata peels as a source of nobiletin and tangeretin using simple ultrasonic-assisted extraction

As the highest yield crop worldwide, citrus peels that possess bioactive compounds were discarded as a futile by-product. Ultrasonication with environmentally friendly solvent (50% ethanol and ddH2O) were used in the present study to extract flavonoids from Citrus depressa Hayata peels with extracti...

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Main Authors: Wei-Jyun Chien, Dinar S. Saputri, Hung-Yu Lin
Format: Article
Language:English
Published: Elsevier 2022-01-01
Series:Current Research in Food Science
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2665927122000132
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author Wei-Jyun Chien
Dinar S. Saputri
Hung-Yu Lin
author_facet Wei-Jyun Chien
Dinar S. Saputri
Hung-Yu Lin
author_sort Wei-Jyun Chien
collection DOAJ
description As the highest yield crop worldwide, citrus peels that possess bioactive compounds were discarded as a futile by-product. Ultrasonication with environmentally friendly solvent (50% ethanol and ddH2O) were used in the present study to extract flavonoids from Citrus depressa Hayata peels with extraction period and fruit maturity as other variables. DPPH scavenging activity was investigated. Qualitative flavonoid content analysis was done by UV/Vis and FTIR-ATR spectra. Quantification of flavonoid using LC-MS/MS found that solvent type, fruit maturity, and ultrasonication period significantly affect the extracted flavonoid yield (p < 0.05). Extraction using 50% ethanol showed a higher yield than ddH2O. Flavonoid content was also higher in unripe than ripe samples. Nobiletin, tangeretin, and rutin were dominant among the identified compounds in all sample treatments. Flavonoid content in Citrus depressa Hayata extract was found to negatively correlate to DPPH scavenging activity, which needs further research to identify other bioactivities of these flavonoids.
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spelling doaj.art-ebd00364f8864ab79eefd670c8b2cc062022-12-22T04:40:32ZengElsevierCurrent Research in Food Science2665-92712022-01-015278287Valorization of Taiwan's Citrus depressa Hayata peels as a source of nobiletin and tangeretin using simple ultrasonic-assisted extractionWei-Jyun Chien0Dinar S. Saputri1Hung-Yu Lin2Department of Applied Chemistry Chaoyang University of Technology Taichung, TaiwanCorresponding author.; Department of Applied Chemistry Chaoyang University of Technology Taichung, TaiwanDepartment of Applied Chemistry Chaoyang University of Technology Taichung, TaiwanAs the highest yield crop worldwide, citrus peels that possess bioactive compounds were discarded as a futile by-product. Ultrasonication with environmentally friendly solvent (50% ethanol and ddH2O) were used in the present study to extract flavonoids from Citrus depressa Hayata peels with extraction period and fruit maturity as other variables. DPPH scavenging activity was investigated. Qualitative flavonoid content analysis was done by UV/Vis and FTIR-ATR spectra. Quantification of flavonoid using LC-MS/MS found that solvent type, fruit maturity, and ultrasonication period significantly affect the extracted flavonoid yield (p < 0.05). Extraction using 50% ethanol showed a higher yield than ddH2O. Flavonoid content was also higher in unripe than ripe samples. Nobiletin, tangeretin, and rutin were dominant among the identified compounds in all sample treatments. Flavonoid content in Citrus depressa Hayata extract was found to negatively correlate to DPPH scavenging activity, which needs further research to identify other bioactivities of these flavonoids.http://www.sciencedirect.com/science/article/pii/S2665927122000132UltrasonicationNobiletinTangeretinFlavonoidLC-MS/MSDPPH
spellingShingle Wei-Jyun Chien
Dinar S. Saputri
Hung-Yu Lin
Valorization of Taiwan's Citrus depressa Hayata peels as a source of nobiletin and tangeretin using simple ultrasonic-assisted extraction
Current Research in Food Science
Ultrasonication
Nobiletin
Tangeretin
Flavonoid
LC-MS/MS
DPPH
title Valorization of Taiwan's Citrus depressa Hayata peels as a source of nobiletin and tangeretin using simple ultrasonic-assisted extraction
title_full Valorization of Taiwan's Citrus depressa Hayata peels as a source of nobiletin and tangeretin using simple ultrasonic-assisted extraction
title_fullStr Valorization of Taiwan's Citrus depressa Hayata peels as a source of nobiletin and tangeretin using simple ultrasonic-assisted extraction
title_full_unstemmed Valorization of Taiwan's Citrus depressa Hayata peels as a source of nobiletin and tangeretin using simple ultrasonic-assisted extraction
title_short Valorization of Taiwan's Citrus depressa Hayata peels as a source of nobiletin and tangeretin using simple ultrasonic-assisted extraction
title_sort valorization of taiwan s citrus depressa hayata peels as a source of nobiletin and tangeretin using simple ultrasonic assisted extraction
topic Ultrasonication
Nobiletin
Tangeretin
Flavonoid
LC-MS/MS
DPPH
url http://www.sciencedirect.com/science/article/pii/S2665927122000132
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AT dinarssaputri valorizationoftaiwanscitrusdepressahayatapeelsasasourceofnobiletinandtangeretinusingsimpleultrasonicassistedextraction
AT hungyulin valorizationoftaiwanscitrusdepressahayatapeelsasasourceofnobiletinandtangeretinusingsimpleultrasonicassistedextraction